Restriction map for pF1KB4697
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       AclI        AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BciVI       
BclI        BcnI        BfaI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsgI        BsiWI       BsmBI       BsmI        BspDI       
BspHI       BspLU11I    BsrBI       BsrDI       BssSI       
Bst1107I    BstBI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HinfI       HpaI        KasI        
MaeI        MamI        MfeI        MluI        MunI        
NarI        NciI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SalI        SapI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SspI        
SwaI        TfiI        Tth111I     Van91I      XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I        710  GGTACC               G^GTACC
AccI          472  GTCTAC               GT^[AC][GT]AC
AccIII        242  TCCGGA               T^CCGGA
AcsI          658  GAATTC               [GA]^AATT[CT]
AflII         664  CTTAAG               C^TTAAG
AflIII        770  ACGTGT               A^C[GA][CT]GT
ApaI          151  GGGCCC               GGGCC^C
ApoI          658  GAATTC               [GA]^AATT[CT]
Asp718I       710  GGTACC               G^GTACC
AspHI         563  GAGCTC               G[AT]GC[AT]^C
BalI           71  TGGCCA               TGG^CCA
BbvCI         336  CCTCAGC              CCTCAGC(-5/-2)
BcgI          425  GCATCCTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          664  CTTAAG               C^TTAAG
BsaI          586  GAGACC               GGTCTC(1/5)
BsaWI         242  TCCGGA               [AT]^CCGG[AT]
BseAI         242  TCCGGA               T^CCGGA
BseRI         298  CTCCTC               GAGGAG(10/8)
BsiHKAI       563  GAGCTC               G[AT]GC[AT]^C
BsmAI         586  GAGAC                GTCTC(1/5)
Bsp1407I      827  TGTACA               T^GTACA
BspEI         242  TCCGGA               T^CCGGA
BspMI         903  GCAGGT               ACCTGC(4/8)
BsrGI         827  TGTACA               T^GTACA
BsrI           95  ACTGG                ACTGG(1/-1)
BssHII        110  GCGCGC               G^CGCGC
BstAPI         24  GCAGATGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         803  CCATCCCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI           41  CGGCCG               C^GGCCG
EclXI          41  CGGCCG               C^GGCCG
Eco52I         41  CGGCCG               C^GGCCG
EcoRI         658  GAATTC               G^AATTC
HaeII         722  GGCGCT               [GA]GCGC^[CT]
KpnI          710  GGTACC               GGTAC^C
MluNI          71  TGGCCA               TGG^CCA
MroI          242  TCCGGA               T^CCGGA
MscI           71  TGGCCA               TGG^CCA
MslI          804  CATCCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          421  GCCGGC               GCC^GGC
NgoMIV        421  GCCGGC               G^CCGGC
NspI          953  GCATGC               [GA]CATG^[CT]
PaeR7I        726  CTCGAG               C^TCGAG
PpuMI         926  AGGACCT              [GA]G^G[AT]CC[CT]
SacI          563  GAGCTC               GAGCT^C
SbfI          900  CCTGCAGG             CCTGCA^GG
SphI          953  GCATGC               GCATG^C
Sse8387I      900  CCTGCAGG             CCTGCA^GG
SspBI         827  TGTACA               T^GTACA
Tsp509I       659  AATT                 ^AATT
XcmI          131  CCAACCTGACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          726  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcyI          595  GGCGTC               G[GA]^CG[CT]C
              669  GGCGTC              
AlwNI          25  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              925  CAGGACCTG           
AvaI          498  CCCGAG               C^[CT]CG[GA]G
              726  CTCGAG              
BsaHI         595  GGCGTC               G[GA]^CG[CT]C
              669  GGCGTC              
BsiEI          12  CGACCG               CG[GA][CT]^CG
               41  CGGCCG              
BsmFI         512  GGGAC                GGGAC(10/14)
              767  GGGAC               
BsoBI         498  CCCGAG               C^[CT]CG[GA]G
              726  CTCGAG              
BsrFI         421  GCCGGC               [GA]^CCGG[CT]
              713  ACCGGC              
BstEII        493  GGTGACC              G^GT[ACGT]ACC
              855  GGTGACC             
BstPI         493  GGTGACC              G^GT[ACGT]ACC
              855  GGTGACC             
BstYI         221  GGATCT               [GA]^GATC[CT]
              386  GGATCT              
Cfr10I        421  GCCGGC               [GA]^CCGG[CT]
              713  ACCGGC              
DraII         151  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              926  AGGACCT             
EaeI           41  CGGCCG               [CT]^GGCC[GA]
               71  TGGCCA              
EarI          430  CTCTTC               CTCTTC(1/4)
              678  CTCTTC              
EcoO109I      151  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              926  AGGACCT             
EcoO65I       493  GGTGACC              G^GT[ACGT]ACC
              855  GGTGACC             
Hin1I         595  GGCGTC               G[GA]^CG[CT]C
              669  GGCGTC              
Ksp632I       430  CTCTTC               CTCTTC(1/4)
              678  CTCTTC              
KspI           15  CCGCGG               CCGC^GG
              171  CCGCGG              
MaeII         770  ACGT                 A^CGT
              947  ACGT                
MflI          221  GGATCT               [GA]^GATC[CT]
              386  GGATCT              
MseI          644  TTAA                 T^TAA
              665  TTAA                
MspA1I         15  CCGCGG               C[AC]G^C[GT]G
              171  CCGCGG              
SacII          15  CCGCGG               CCGC^GG
              171  CCGCGG              
StuI          444  AGGCCT               AGG^CCT
              752  AGGCCT              
TaqI          215  TCGA                 T^CGA
              727  TCGA                
Tru9I         644  TTAA                 T^TAA
              665  TTAA                
TspRI         235  CACTG                CA[GC]TG[ACGT][ACGT]^
              417  CAGTG               
TthHB8I       215  TCGA                 T^CGA
              727  TCGA                
XhoII         221  GGATCT               [GA]^GATC[CT]
              386  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaII       BanI        BanII       BbsI        BbvI        
BmyI        BpmI        BpuAI       BsaJI       BsiYI       
BslI        Bsp1286I    BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DsaI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MspI        
MvaI        MvnI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TseI        Tsp45I      
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