Restriction map for pF1KB4651
##################################
# NO CUT ENZYMES (  175 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cac8I       CelII       Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     KasI        KpnI        Ksp632I     
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StyI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccI          137  GTCTAC               GT^[AC][GT]AC
AciI          798  CCGC                 CCGC(-3/-1)
AfaI          176  GTAC                 GT^AC
AlwNI          22  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BanII         635  GAGCCC               G[GA]GC[CT]^C
BbvCI         583  GCTGAGG              CCTCAGC(-5/-2)
BglI          181  GCCAGAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          635  GAGCCC               G[AGT]GC[ACT]^C
BsaI           90  GAGACC               GGTCTC(1/5)
BsaJI         550  CCAGGG               C^C[ACGT][ACGT]GG
BsgI          102  GTGCAG               GTGCAG(16/14)
Bsp1286I      635  GAGCCC               G[AGT]GC[ACT]^C
BsrDI         203  GCAATG               GCAATG(2/0)
BsrI          241  CCAGT                ACTGG(1/-1)
CfoI          507  GCGC                 GCG^C
EcoRI         575  GAATTC               G^AATTC
HapII           4  CCGG                 C^CGG
HhaI          507  GCGC                 GCG^C
HinP1I        507  GCGC                 G^CGC
HincII        691  GTTAAC               GT[CT]^[GA]AC
HindII        691  GTTAAC               GT[CT]^[GA]AC
HpaI          691  GTTAAC               GTT^AAC
HpaII           4  CCGG                 C^CGG
MaeII         251  ACGT                 A^CGT
MslI           95  CATGCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI            4  CCGG                 C^CGG
NspI          190  GCATGT               [GA]CATG^[CT]
PstI          299  CTGCAG               CTGCA^G
RsaI          176  GTAC                 GT^AC
SspI          779  AATATT               AAT^ATT
StuI          131  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AvaII           6  GGACC                G^G[AT]CC
              238  GGTCC               
BsiYI         229  CCAAAATTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              550  CCAGGGATAGG         
BslI          229  CCAAAATTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              550  CCAGGGATAGG         
BsmAI          90  GAGAC                GTCTC(1/5)
              610  GTCTC               
BspMI          98  GCAGGT               ACCTGC(4/8)
              366  GCAGGT              
BssKI         129  CCAGG                ^CC[ACGT]GG
              550  CCAGG               
BstF5I        153  GGATG                GGATG(2/0)
              393  GGATG               
BstNI         129  CCAGG                CC^[AT]GG
              550  CCAGG               
BstYI         109  GGATCT               [GA]^GATC[CT]
              258  GGATCT              
Cfr13I          6  GGACC                G^G[ACGT]CC
              238  GGTCC               
DraI          534  TTTAAA               TTT^AAA
              754  TTTAAA              
EcoRII        129  CCAGG                ^CC[AT]GG
              550  CCAGG               
FokI          153  GGATG                GGATG(9/13)
              393  GGATG               
HaeIII        132  GGCC                 GG^CC
              588  GGCC                
HinfI         474  GATTC                G^A[ACGT]TC
              623  GAATC               
HphI           87  GGTGA                GGTGA(8/7)
              126  TCACC               
MaeIII        340  GTGAC                ^GT[ACGT]AC
              355  GTGAC               
MboII          64  GAAGA                GAAGA(8/7)
              826  GAAGA               
MflI          109  GGATCT               [GA]^GATC[CT]
              258  GGATCT              
MvaI          129  CCAGG                CC^[AT]GG
              550  CCAGG               
MwoI          181  GCCAGAGTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              730  GCGTTTGTTGC         
NlaIV         237  GGGTCC               GG[ACGT]^[ACGT]CC
              634  GGAGCC              
Sau96I          6  GGACC                G^G[ACGT]CC
              238  GGTCC               
ScrFI         129  CCAGG                CC^[ACGT]GG
              550  CCAGG               
SfaNI          24  GATGC                GCATC(5/9)
              363  GATGC               
SfcI           16  CTACAG               C^T[GA][CT]AG
              299  CTGCAG              
TfiI          474  GATTC                G^A[AT]TC
              623  GAATC               
Tsp45I        340  GTGAC                ^GT[GC]AC
              355  GTGAC               
XhoII         109  GGATCT               [GA]^GATC[CT]
              258  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BbvI        
DdeI        DpnII       Fnu4HI      ItaI        MboI        
MnlI        MseI        MspA1I      NdeII       NlaIII      
PvuII       Sau3AI      TaqI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     
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