Restriction map for pF1KB4616
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BsaBI       
BsaHI       BsaI        BsgI        BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MslI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII          18  CGCG                 CG^CG
AccIII        537  TCCGGA               T^CCGGA
AfaI          560  GTAC                 GT^AC
AflIII         49  ACGTGT               A^C[GA][CT]GT
AspHI         732  GAGCTC               G[AT]GC[AT]^C
AvaI          752  CTCGGG               C^[CT]CG[GA]G
BanI         1009  GGTGCC               G^G[CT][GA]CC
BbrPI          48  CACGTG               CAC^GTG
BbvI          582  GCAGC                GCAGC(8/12)
BcnI           79  CCGGG                CC^[GC]GG
BlpI          970  GCTGAGC              GC^T[ACGT]AGC
BpmI            8  CTGGAG               CTGGAG(16/14)
Bpu1102I      970  GCTGAGC              GC^T[ACGT]AGC
BsaAI          48  CACGTG               [CT]AC^GT[GA]
BsaWI         537  TCCGGA               [AT]^CCGG[AT]
BseAI         537  TCCGGA               T^CCGGA
BsiHKAI       732  GAGCTC               G[AT]GC[AT]^C
BsmAI         334  GAGAC                GTCTC(1/5)
BsmFI         755  GGGAC                GGGAC(10/14)
BsmI          103  GAATGC               GAATGC(1/-1)
BsoBI         752  CTCGGG               C^[CT]CG[GA]G
BspEI         537  TCCGGA               T^CCGGA
BsrDI         856  GCAATG               GCAATG(2/0)
BstUI          18  CGCG                 CG^CG
CelII         970  GCTGAGC              GC^T[ACGT]AGC
DpnII         702  GATC                 ^GATC
MboI          702  GATC                 ^GATC
MroI          537  TCCGGA               T^CCGGA
MvnI           18  CGCG                 CG^CG
NciI           79  CCGGG                CC^[GC]GG
NcoI          543  CCATGG               C^CATGG
NdeII         702  GATC                 ^GATC
PflMI         604  CCAGTCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI          48  CACGTG               CAC^GTG
PmlI           48  CACGTG               CAC^GTG
PpuMI         999  AGGACCC              [GA]G^G[AT]CC[CT]
RsaI          560  GTAC                 GT^AC
SacI          732  GAGCTC               GAGCT^C
Sau3AI        702  GATC                 ^GATC
StuI          194  AGGCCT               AGG^CCT
TseI          582  GCAGC                G^C[AT]GC
Van91I        604  CCAGTCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcsI          484  GAATTT               [GA]^AATT[CT]
              784  GAATTT              
ApoI          484  GAATTT               [GA]^AATT[CT]
              784  GAATTT              
BalI          521  TGGCCA               TGG^CCA
              860  TGGCCA              
BbsI          379  GAAGAC               GAAGAC(2/6)
              427  GAAGAC              
BfaI          643  CTAG                 C^TAG
              838  CTAG                
BpuAI         379  GAAGAC               GAAGAC(2/6)
              427  GAAGAC              
BseRI         367  GAGGAG               GAGGAG(10/8)
              817  GAGGAG              
Cac8I          62  GCGAGC               GC[ACGT]^[ACGT]GC
              207  GCCAGC              
CfoI           17  GCGC                 GCG^C
              139  GCGC                
DraII         827  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              999  AGGACCC             
DsaI          183  CCACGG               C^C[GA][CT]GG
              543  CCATGG              
EcoO109I      827  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              999  AGGACCC             
EcoT14I       543  CCATGG               C^C[AT][AT]GG
              938  CCAAGG              
FauI          204  CCCGC                CCCGC(4/6)
              226  GCGGG               
HapII          79  CCGG                 C^CGG
              538  CCGG                
HhaI           17  GCGC                 GCG^C
              139  GCGC                
HinP1I         17  GCGC                 G^CGC
              139  GCGC                
HpaII          79  CCGG                 C^CGG
              538  CCGG                
HphI          162  TCACC                GGTGA(8/7)
              618  TCACC               
MaeI          643  CTAG                 C^TAG
              838  CTAG                
MaeII          49  ACGT                 A^CGT
              179  ACGT                
MluNI         521  TGGCCA               TGG^CCA
              860  TGGCCA              
MscI          521  TGGCCA               TGG^CCA
              860  TGGCCA              
MseI          664  TTAA                 T^TAA
              788  TTAA                
MspA1I        209  CAGCTG               C[AC]G^C[GT]G
              968  CAGCTG              
MspI           79  CCGG                 C^CGG
              538  CCGG                
PvuII         209  CAGCTG               CAG^CTG
              968  CAGCTG              
StyI          543  CCATGG               C^C[AT][AT]GG
              938  CCAAGG              
TaqI           26  TCGA                 T^CGA
              704  TCGA                
TfiI          448  GAATC                G^A[AT]TC
              964  GAATC               
Tru9I         664  TTAA                 T^TAA
              788  TTAA                
TspRI        1005  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1014  CAGTG               
TthHB8I        26  TCGA                 T^CGA
              704  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
EaeI        EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        Ksp632I     MaeIII      
MboII       MnlI        MvaI        MwoI        NlaIII      
NlaIV       PleI        Sau96I      ScrFI       Tsp45I      
Tsp509I     
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