Restriction map for pF1KB4392
 (Linear) (Six-base) MAPSORT of: KIBB4392.seq  Check: 8602  from: 1  to: 1068



KIBB4392.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     716    1068 
   Size:        716     352
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572    1068 
   Size:        572     496
 
AfeI AGC'GCT

Cuts at:      0     159    1068 
   Size:        159     909
 
AgeI A'CCGG_T

Cuts at:      0     623    1068 
   Size:        623     445
 
AleI CACnn'nnGTG

Cuts at:      0     441    1068 
   Size:        441     627
 
AlwNI CAG_nnn'CTG

Cuts at:      0      37    1068 
   Size:         37    1031
 
AvaI C'yCGr_G

Cuts at:      0      82     119     413     452     771     827     911     941 
   Size:         82      37     294      39     319      56      84      30

Cuts at:    941     971    1060    1068 
   Size:         30      89       8

  Fragments arranged by size: 

                 319     294      89      84      82      56      39      37
                  30      30       8
 
BanI G'GyrC_C

Cuts at:      0     231     756    1068 
   Size:        231     525     312

  Fragments arranged by size: 

                 525     312     231
 
BanII G_rGCy'C

Cuts at:      0     616    1068 
   Size:        616     452
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     276    1068 
   Size:        276     792
 
BbvCI CC'TCA_GC

Cuts at:      0     299     731     790    1068 
   Size:        299     432      59     278

  Fragments arranged by size: 

                 432     299     278      59
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     314     930    1068 
   Size:        314     616     138

  Fragments arranged by size: 

                 616     314     138
 
BglII A'GATC_T

Cuts at:      0     175    1068 
   Size:        175     893
 
BlpI GC'TnA_GC

Cuts at:      0     542    1068 
   Size:        542     526
 
Bme1580I G_kGCm'C

Cuts at:      0     761    1068 
   Size:        761     307
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     176     986    1068 
   Size:        176     810      82

  Fragments arranged by size: 

                 810     176      82
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     750    1011    1068 
   Size:        628     122     261      57

  Fragments arranged by size: 

                 628     261     122      57
 
Bpu10I CC'TnA_GC

Cuts at:      0     299     731     790    1068 
   Size:        299     432      59     278

  Fragments arranged by size: 

                 432     299     278      59
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     850    1068 
   Size:        313     537     218

  Fragments arranged by size: 

                 537     313     218
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     632     866     957    1068 
   Size:        632     234      91     111

  Fragments arranged by size: 

                 632     234     111      91
 
BsaWI w'CCGG_w

Cuts at:      0     317     623    1068 
   Size:        317     306     445

  Fragments arranged by size: 

                 445     317     306
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     404    1012    1027    1068 
   Size:        404     608      15      41

  Fragments arranged by size: 

                 608     404      41      15
 
BseYI C'CCAG_C

Cuts at:      0      30      43     394     800     819     905     927     952 
   Size:         30      13     351     406      19      86      22      25

Cuts at:    952    1068 
   Size:        116

  Fragments arranged by size: 

                 406     351     116      86      30      25      22      19
                  13
 
Bsp1286I G_dGCh'C

Cuts at:      0     616     761    1068 
   Size:        616     145     307

  Fragments arranged by size: 

                 616     307     145
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     198     356     716    1068 
   Size:        198     158     360     352

  Fragments arranged by size: 

                 360     352     198     158
 
BsrBI CCG'CTC

Cuts at:      0     457    1035    1068 
   Size:        457     578      33

  Fragments arranged by size: 

                 578     457      33
 
BsrFI r'CCGG_y

Cuts at:      0     623    1068 
   Size:        623     445
 
BstYI r'GATC_y

Cuts at:      0     175     286    1038    1068 
   Size:        175     111     752      30

  Fragments arranged by size: 

                 752     175     111      30
 
Bsu36I CC'TnA_GG

Cuts at:      0     569    1068 
   Size:        569     499
 
BtgI C'CryG_G

Cuts at:      0     492     597    1068 
   Size:        492     105     471

  Fragments arranged by size: 

                 492     471     105
 
BtsI GCAGTG_nn'

Cuts at:      0     254     698    1068 
   Size:        254     444     370

  Fragments arranged by size: 

                 444     370     254
 
EaeI y'GGCC_r

Cuts at:      0     977    1068 
   Size:        977      91
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572     628     750    1011    1068 
   Size:        572      56     122     261      57

  Fragments arranged by size: 

                 572     261     122      57      56
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      55    1068 
   Size:         55    1013
 
EcoO109I rG'GnC_Cy

Cuts at:      0     135     345     727     823    1068 
   Size:        135     210     382      96     245

  Fragments arranged by size: 

                 382     245     210     135      96
 
HaeII r_GCGC'y

Cuts at:      0     161    1068 
   Size:        161     907
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     707    1068 
   Size:        707     361
 
MscI TGG'CCA

Cuts at:      0     979    1068 
   Size:        979      89
 
MslI CAynn'nnrTG

Cuts at:      0     441    1068 
   Size:        441     627
 
MspA1I CmG'CkG

Cuts at:      0     472    1068 
   Size:        472     596
 
NcoI C'CATG_G

Cuts at:      0     597    1068 
   Size:        597     471
 
PpuMI rG'GwC_Cy

Cuts at:      0     345     727     823    1068 
   Size:        345     382      96     245

  Fragments arranged by size: 

                 382     345     245      96
 
PspXI vC'TCGA_Gb

Cuts at:      0     452    1060    1068 
   Size:        452     608       8

  Fragments arranged by size: 

                 608     452       8
 
PstI C_TGCA'G

Cuts at:      0     117    1068 
   Size:        117     951
 
PvuII CAG'CTG

Cuts at:      0     472    1068 
   Size:        472     596
 
SanDI GG'GwC_CC

Cuts at:      0     823    1068 
   Size:        823     245
 
ScaI AGT'ACT

Cuts at:      0     649    1068 
   Size:        649     419
 
SfcI C'TryA_G

Cuts at:      0     113     891    1068 
   Size:        113     778     177

  Fragments arranged by size: 

                 778     177     113
 
SmaI CCC'GGG

Cuts at:      0     415     773     829    1068 
   Size:        415     358      56     239

  Fragments arranged by size: 

                 415     358     239      56
 
SmlI C'TyrA_G

Cuts at:      0     292     452     865    1060    1068 
   Size:        292     160     413     195       8

  Fragments arranged by size: 

                 413     292     195     160       8
 
SrfI GCCC'GGGC

Cuts at:      0     773    1068 
   Size:        773     295
 
StyI C'CwwG_G

Cuts at:      0     597    1068 
   Size:        597     471
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     495     682    1068 
   Size:        495     187     386

  Fragments arranged by size: 

                 495     386     187
 
TatI w'GTAC_w

Cuts at:      0     647    1068 
   Size:        647     421
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     679     711    1068 
   Size:        679      32     357

  Fragments arranged by size: 

                 679     357      32
 
XhoI C'TCGA_G

Cuts at:      0     452    1060    1068 
   Size:        452     608       8

  Fragments arranged by size: 

                 608     452       8
 
XmaI C'CCGG_G

Cuts at:      0     413     771     827    1068 
   Size:        413     358      56     241

  Fragments arranged by size: 

                 413     358     241      56
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AgeI     AleI    AlwNI     AvaI     BanI
    BanII     BbsI    BbvCI    BciVI    BglII     BlpI Bme1580I     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BseRI    BseYI Bsp1286I
    BspMI    BsrBI    BsrFI    BstYI   Bsu36I     BtgI     BtsI     EaeI
  Eco57MI    EcoNI EcoO109I    HaeII     MmeI     MscI     MslI   MspA1I
     NcoI    PpuMI    PspXI     PstI    PvuII    SanDI     ScaI     SfcI
     SmaI     SmlI     SrfI     StyI    TaqII     TatI     TstI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AhdI     AlfI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BcgI     BclI    BfrBI     BglI    BmgBI
     BmrI     BmtI    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII     SacI    SacII
     SalI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SspI     StuI     SwaI  Tth111I     XbaI
     XcmI     XmnI     ZraI
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