Restriction map for pF1KB4334
 (Linear) (Six-base) MAPSORT of: KIBB4334.seq  Check: 3226  from: 1  to: 1419



KIBB4334.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     727    1419 
   Size:        727     692
 
AflIII A'CryG_T

Cuts at:      0      98     920    1419 
   Size:         98     822     499

  Fragments arranged by size: 

                 822     499      98
 
AhdI GACnn_n'nnGTC

Cuts at:      0     381    1350    1419 
   Size:        381     969      69

  Fragments arranged by size: 

                 969     381      69
 
AleI CACnn'nnGTG

Cuts at:      0     844    1419 
   Size:        844     575
 
ApoI r'AATT_y

Cuts at:      0    1167    1419 
   Size:       1167     252
 
AvaI C'yCGr_G

Cuts at:      0      91     657     832    1223    1419 
   Size:         91     566     175     391     196

  Fragments arranged by size: 

                 566     391     196     175      91
 
BamHI G'GATC_C

Cuts at:      0     302    1419 
   Size:        302    1117
 
BanI G'GyrC_C

Cuts at:      0     351     682    1419 
   Size:        351     331     737

  Fragments arranged by size: 

                 737     351     331
 
BanII G_rGCy'C

Cuts at:      0     458     623    1419 
   Size:        458     165     796

  Fragments arranged by size: 

                 796     458     165
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     418     663    1279    1286    1419 
   Size:        418     245     616       7     133

  Fragments arranged by size: 

                 616     418     245     133       7
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1318    1352    1419 
   Size:       1318      34      67

  Fragments arranged by size: 

                1318      67      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     724    1419 
   Size:        724     695
 
BfrBI ATG'CAT

Cuts at:      0     482     811    1419 
   Size:        482     329     608

  Fragments arranged by size: 

                 608     482     329
 
Bme1580I G_kGCm'C

Cuts at:      0     356    1419 
   Size:        356    1063
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     847    1238    1419 
   Size:        847     391     181

  Fragments arranged by size: 

                 847     391     181
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     985    1419 
   Size:        985     434
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     660     877    1146    1419 
   Size:        660     217     269     273

  Fragments arranged by size: 

                 660     273     269     217
 
BsaBI GATnn'nnATC

Cuts at:      0     196    1419 
   Size:        196    1223
 
BsaWI w'CCGG_w

Cuts at:      0     601    1419 
   Size:        601     818
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     389     419    1419 
   Size:        389      30    1000

  Fragments arranged by size: 

                1000     389      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1302    1419 
   Size:       1302     117
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     507    1419 
   Size:        507     912
 
BsiHKAI G_wGCw'C

Cuts at:      0     623     647    1419 
   Size:        623      24     772

  Fragments arranged by size: 

                 772     623      24
 
BsmI GAATG_Cn'

Cuts at:      0    1341    1419 
   Size:       1341      78
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     109    1419 
   Size:        109    1310
 
Bsp1286I G_dGCh'C

Cuts at:      0     356     458     623     647    1419 
   Size:        356     102     165      24     772

  Fragments arranged by size: 

                 772     356     165     102      24
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     817    1419 
   Size:        817     602
 
BstYI r'GATC_y

Cuts at:      0     302    1419 
   Size:        302    1117
 
Bsu36I CC'TnA_GG

Cuts at:      0     493    1419 
   Size:        493     926
 
BtgI C'CryG_G

Cuts at:      0     554    1419 
   Size:        554     865
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     189    1094    1419 
   Size:        189     905     325

  Fragments arranged by size: 

                 905     325     189
 
EarI CTCTTCn'nnn_

Cuts at:      0     712     993    1419 
   Size:        712     281     426

  Fragments arranged by size: 

                 712     426     281
 
EcoICRI GAG'CTC

Cuts at:      0     621    1419 
   Size:        621     798
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     727     985    1419 
   Size:        727     258     434

  Fragments arranged by size: 

                 727     434     258
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1226    1419 
   Size:       1226     193
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6     172    1003    1419 
   Size:          6     166     831     416

  Fragments arranged by size: 

                 831     416     166       6
 
EcoRI G'AATT_C

Cuts at:      0    1167    1419 
   Size:       1167     252
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     367     399    1336    1368    1419 
   Size:        367      32     937      32      51

  Fragments arranged by size: 

                 937     367      51      32      32
 
HincII GTy'rAC

Cuts at:      0     234     981    1106    1416    1419 
   Size:        234     747     125     310       3

  Fragments arranged by size: 

                 747     310     234     125       3
 
HindIII A'AGCT_T

Cuts at:      0    1021    1419 
   Size:       1021     398
 
HpaI GTT'AAC

Cuts at:      0    1416    1419 
   Size:       1416       3
 
MslI CAynn'nnrTG

Cuts at:      0     217     434     844     919    1419 
   Size:        217     217     410      75     500

  Fragments arranged by size: 

                 500     410     217     217      75
 
NsiI A_TGCA'T

Cuts at:      0     484     813    1419 
   Size:        484     329     606

  Fragments arranged by size: 

                 606     484     329
 
NspI r_CATG'y

Cuts at:      0     102     482     924    1221    1419 
   Size:        102     380     442     297     198

  Fragments arranged by size: 

                 442     380     297     198     102
 
PciI A'CATG_T

Cuts at:      0      98     920    1419 
   Size:         98     822     499

  Fragments arranged by size: 

                 822     499      98
 
PfoI T'CCnGG_A

Cuts at:      0    1354    1419 
   Size:       1354      65
 
PpuMI rG'GwC_Cy

Cuts at:      0     172    1419 
   Size:        172    1247
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     715     747    1419 
   Size:        715      32     672

  Fragments arranged by size: 

                 715     672      32
 
SacI G_AGCT'C

Cuts at:      0     623    1419 
   Size:        623     796
 
SanDI GG'GwC_CC

Cuts at:      0     172    1419 
   Size:        172    1247
 
SmaI CCC'GGG

Cuts at:      0      93    1419 
   Size:         93    1326
 
SmlI C'TyrA_G

Cuts at:      0     657    1223    1419 
   Size:        657     566     196

  Fragments arranged by size: 

                 657     566     196
 
SphI G_CATG'C

Cuts at:      0     482    1221    1419 
   Size:        482     739     198

  Fragments arranged by size: 

                 739     482     198
 
SspI AAT'ATT

Cuts at:      0     271    1098    1419 
   Size:        271     827     321

  Fragments arranged by size: 

                 827     321     271
 
StyI C'CwwG_G

Cuts at:      0    1040    1419 
   Size:       1040     379
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     339     774     886    1419 
   Size:        339     435     112     533

  Fragments arranged by size: 

                 533     435     339     112
 
TatI w'GTAC_w

Cuts at:      0    1202    1406    1419 
   Size:       1202     204      13

  Fragments arranged by size: 

                1202     204      13
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     389     421    1419 
   Size:        389      32     998

  Fragments arranged by size: 

                 998     389      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     216    1419 
   Size:        216    1203
 
XhoI C'TCGA_G

Cuts at:      0     657    1223    1419 
   Size:        657     566     196

  Fragments arranged by size: 

                 657     566     196
 
XmaI C'CCGG_G

Cuts at:      0      91    1419 
   Size:         91    1328
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AleI     ApoI     AvaI    BamHI     BanI
    BanII     BbsI     BcgI    BciVI    BfrBI Bme1580I     BplI     BpmI
     BsaI    BsaBI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I   BstAPI    BstYI   Bsu36I     BtgI    BtgZI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I   HincII  HindIII
     HpaI     MslI     NsiI     NspI     PciI     PfoI    PpuMI     PsrI
     SacI    SanDI     SmaI     SmlI     SphI     SspI     StyI    TaqII
     TatI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI    BbvCI     BclI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI    BsaAI    BsaHI    BseYI
    BsiEI    BsiWI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PstI
     PvuI    PvuII    RsrII    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     StuI     SwaI  Tth111I     XbaI     XmnI     ZraI
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