(Linear) (Six-base) MAPSORT of: KIBB4278.seq  Check: 3310  from: 1  to: 1032



KIBB4278.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AccI GT'mk_AC

Cuts at:      0      68    1032 
   Size:         68     964
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503    1032 
   Size:        503     529
 
AflII C'TTAA_G

Cuts at:      0     793    1032 
   Size:        793     239
 
ApaLI G'TGCA_C

Cuts at:      0     981    1032 
   Size:        981      51
 
ApoI r'AATT_y

Cuts at:      0     674    1032 
   Size:        674     358
 
AseI AT'TA_AT

Cuts at:      0     707    1032 
   Size:        707     325
 
AvaI C'yCGr_G

Cuts at:      0      52      98    1032 
   Size:         52      46     934

  Fragments arranged by size: 

                 934      52      46
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     350    1032 
   Size:        350     682
 
BclI T'GATC_A

Cuts at:      0     972    1032 
   Size:        972      60
 
BglII A'GATC_T

Cuts at:      0     789    1032 
   Size:        789     243
 
BlpI GC'TnA_GC

Cuts at:      0     560    1032 
   Size:        560     472
 
Bme1580I G_kGCm'C

Cuts at:      0     985    1032 
   Size:        985      47
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     962    1032 
   Size:        962      70
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     750    1032 
   Size:        750     282
 
Bpu10I CC'TnA_GC

Cuts at:      0     863    1032 
   Size:        863     169
 
BsaWI w'CCGG_w

Cuts at:      0     377    1032 
   Size:        377     655
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     953     958    1032 
   Size:        953       5      74

  Fragments arranged by size: 

                 953      74       5
 
BsiHKAI G_wGCw'C

Cuts at:      0      23     985    1032 
   Size:         23     962      47

  Fragments arranged by size: 

                 962      47      23
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     985    1032 
   Size:         23     962      47

  Fragments arranged by size: 

                 962      47      23
 
BspEI T'CCGG_A

Cuts at:      0     377    1032 
   Size:        377     655
 
BspHI T'CATG_A

Cuts at:      0      33     366    1032 
   Size:         33     333     666

  Fragments arranged by size: 

                 666     333      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      19    1032 
   Size:         19    1013
 
BstYI r'GATC_y

Cuts at:      0     211     789    1032 
   Size:        211     578     243

  Fragments arranged by size: 

                 578     243     211
 
Bsu36I CC'TnA_GG

Cuts at:      0     881    1032 
   Size:        881     151
 
DraIII CAC_nnn'GTG

Cuts at:      0     152    1032 
   Size:        152     880
 
EarI CTCTTCn'nnn_

Cuts at:      0      77    1032 
   Size:         77     955
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     750    1032 
   Size:        503     247     282

  Fragments arranged by size: 

                 503     282     247
 
HindIII A'AGCT_T

Cuts at:      0     569    1032 
   Size:        569     463
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      69     117    1032 
   Size:         69      48     915

  Fragments arranged by size: 

                 915      69      48
 
MspA1I CmG'CkG

Cuts at:      0     564    1032 
   Size:        564     468
 
PfoI T'CCnGG_A

Cuts at:      0     156    1032 
   Size:        156     876
 
PvuII CAG'CTG

Cuts at:      0     564    1032 
   Size:        564     468
 
SfcI C'TryA_G

Cuts at:      0     852    1032 
   Size:        852     180
 
SmlI C'TyrA_G

Cuts at:      0      98     793    1032 
   Size:         98     695     239

  Fragments arranged by size: 

                 695     239      98
 
SspI AAT'ATT

Cuts at:      0     246     354    1032 
   Size:        246     108     678

  Fragments arranged by size: 

                 678     246     108
 
TatI w'GTAC_w

Cuts at:      0     397    1032 
   Size:        397     635
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     138     170    1032 
   Size:        138      32     862

  Fragments arranged by size: 

                 862     138      32
 
XhoI C'TCGA_G

Cuts at:      0      98    1032 
   Size:         98     934
 

 Enzymes that do cut:

     AccI     AcuI    AflII    ApaLI     ApoI     AseI     AvaI     BbsI
     BclI    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BsaWI
    BseRI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BstYI   Bsu36I
   DraIII     EarI  Eco57MI  HindIII     MmeI   MspA1I     PfoI    PvuII
     SfcI     SmlI     SspI     TatI     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI    AsiSI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI