Restriction map for pF1KB4266
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AgeI        AhdI        Alw44I      AlwNI       AocI        
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AvrII       BamHI       BbrPI       
BbvCI       BciVI       BfrI        BglII       BlnI        
BsaAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     HincII      
HindII      HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MroI        MunI        NdeI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       PstI        PvuI        RcaI        RsrII       
SacI        SalI        SbfI        SexAI       SfcI        
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        TfiI        XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AclI          676  AACGTT               AA^CGTT
AcsI         1481  AAATTT               [GA]^AATT[CT]
AlwI         1399  GGATC                GGATC(4/5)
Aor51HI       638  AGCGCT               AGC^GCT
ApoI         1481  AAATTT               [GA]^AATT[CT]
Asp700I      1308  GAACAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1227  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          423  CTCGGG               C^[CT]CG[GA]G
BalI          851  TGGCCA               TGG^CCA
BanI          506  GGCGCC               G^G[CT][GA]CC
BbeI          506  GGCGCC               GGCGC^C
BbsI          187  GTCTTC               GAAGAC(2/6)
BcgI           92  GCACGCGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI           32  TGATCA               T^GATCA
BfaI         1019  CTAG                 C^TAG
BpuAI         187  GTCTTC               GAAGAC(2/6)
BsgI          226  CTGCAC               GTGCAG(16/14)
BsiEI         171  CGACCG               CG[GA][CT]^CG
BsmBI         718  GAGACG               CGTCTC(1/5)
BsoBI         423  CTCGGG               C^[CT]CG[GA]G
BspMI        1501  GCAGGT               ACCTGC(4/8)
BsrDI         683  GCAATG               GCAATG(2/0)
BsrFI         488  GCCGGC               [GA]^CCGG[CT]
BsrI         1133  CCAGT                ACTGG(1/-1)
BssSI         634  CACGAG               CACGAG(-5/-1)
BstEII       1185  GGTCACC              G^GT[ACGT]ACC
BstPI        1185  GGTCACC              G^GT[ACGT]ACC
BstXI         455  CCAAGCGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1399  GGATCT               [GA]^GATC[CT]
Cfr10I        488  GCCGGC               [GA]^CCGG[CT]
DraIII        979  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      638  AGCGCT               AGC^GCT
EcoNI        1513  CCTCTTAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1185  GGTCACC              G^GT[ACGT]ACC
FbaI           32  TGATCA               T^GATCA
FseI          487  GGCCGGCC             GGCCGG^CC
KasI          506  GGCGCC               G^GCGCC
KspI         1381  CCGCGG               CCGC^GG
MaeI         1019  CTAG                 C^TAG
MflI         1399  GGATCT               [GA]^GATC[CT]
MluNI         851  TGGCCA               TGG^CCA
MscI          851  TGGCCA               TGG^CCA
NaeI          488  GCCGGC               GCC^GGC
NarI          506  GGCGCC               GG^CGCC
NcoI         1337  CCATGG               C^CATGG
NgoMIV        488  GCCGGC               G^CCGGC
NheI         1018  GCTAGC               G^CTAGC
PflFI        1227  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         978  CCACTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1456  GACACATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      676  AACGTT               AA^CGTT
PvuII        1393  CAGCTG               CAG^CTG
SacII        1381  CCGCGG               CCGC^GG
SapI          534  GCTCTTC              GCTCTTC(1/4)
ScaI         1444  AGTACT               AGT^ACT
SfoI          506  GGCGCC               GGC^GCC
SphI         1238  GCATGC               GCATG^C
StuI          725  AGGCCT               AGG^CCT
Tth111I      1227  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        978  CCACTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1399  GGATCT               [GA]^GATC[CT]
XmnI         1308  GAACAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AspHI        1138  GAGCAC               G[AT]GC[AT]^C
             1273  GTGCTC              
BlpI          882  GCTGAGC              GC^T[ACGT]AGC
             1342  GCTAAGC             
Bpu1102I      882  GCTGAGC              GC^T[ACGT]AGC
             1342  GCTAAGC             
BsiHKAI      1138  GAGCAC               G[AT]GC[AT]^C
             1273  GTGCTC              
BsmFI          11  GGGAC                GGGAC(10/14)
              555  GTCCC               
BspLU11I     1214  ACATGT               A^CATGT
             1459  ACATGT              
CelII         882  GCTGAGC              GC^T[ACGT]AGC
             1342  GCTAAGC             
DraII         542  AGGACCC              [GA]G^G[ACGT]CC[CT]
              995  GGGTCCT             
EarI          535  CTCTTC               CTCTTC(1/4)
              589  CTCTTC              
EcoO109I      542  AGGACCC              [GA]G^G[ACGT]CC[CT]
              995  GGGTCCT             
HinfI         421  GACTC                G^A[ACGT]TC
              475  GACTC               
Ksp632I       535  CTCTTC               CTCTTC(1/4)
              589  CTCTTC              
PleI          421  GACTC                GAGTC(4/5)
              475  GACTC               
PpuMI         542  AGGACCC              [GA]G^G[AT]CC[CT]
              995  GGGTCCT             


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AvaII       AviII       BanII       BbvI        
BcnI        BglI        BmyI        BpmI        BsaHI       
BsaI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrBI       BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        
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