Restriction map for pF1KB4266
 (Linear) (Six-base) MAPSORT of: KIBB4266.seq  Check: 9986  from: 1  to: 1533



KIBB4266.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AclI AA'CG_TT

Cuts at:      0     677    1533 
   Size:        677     856
 
AfeI AGC'GCT

Cuts at:      0     640    1533 
   Size:        640     893
 
AflIII A'CryG_T

Cuts at:      0     242    1214    1459    1533 
   Size:        242     972     245      74

  Fragments arranged by size: 

                 972     245     242      74
 
AleI CACnn'nnGTG

Cuts at:      0     694    1533 
   Size:        694     839
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     712    1533 
   Size:        712     821
 
ApoI r'AATT_y

Cuts at:      0    1481    1533 
   Size:       1481      52
 
AvaI C'yCGr_G

Cuts at:      0     423    1533 
   Size:        423    1110
 
BanI G'GyrC_C

Cuts at:      0     506    1533 
   Size:        506    1027
 
BanII G_rGCy'C

Cuts at:      0     119     308    1254    1533 
   Size:        119     189     946     279

  Fragments arranged by size: 

                 946     279     189     119
 
BbeI G_GCGC'C

Cuts at:      0     510    1533 
   Size:        510    1023
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     180    1533 
   Size:        180    1353
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      81     115    1533 
   Size:         81      34    1418

  Fragments arranged by size: 

                1418      81      34
 
BclI T'GATC_A

Cuts at:      0      32    1533 
   Size:         32    1501
 
BglI GCCn_nnn'nGGC

Cuts at:      0     109     182     349     494    1533 
   Size:        109      73     167     145    1039

  Fragments arranged by size: 

                1039     167     145     109      73
 
BlpI GC'TnA_GC

Cuts at:      0     883    1343    1533 
   Size:        883     460     190

  Fragments arranged by size: 

                 883     460     190
 
Bme1580I G_kGCm'C

Cuts at:      0    1113    1298    1533 
   Size:       1113     185     235

  Fragments arranged by size: 

                1113     235     185
 
BmgBI CAC'GTC

Cuts at:      0     243     300     915    1229    1533 
   Size:        243      57     615     314     304

  Fragments arranged by size: 

                 615     314     304     243      57
 
BmtI G_CTAG'C

Cuts at:      0    1022    1533 
   Size:       1022     511
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     646     892    1533 
   Size:        466     180     246     641

  Fragments arranged by size: 

                 641     466     246     180
 
Bpu10I CC'TnA_GC

Cuts at:      0    1332    1533 
   Size:       1332     201
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     286     609     622    1533 
   Size:        286     323      13     911

  Fragments arranged by size: 

                 911     323     286      13
 
BsaHI Gr'CG_yC

Cuts at:      0     122     185     341     404     507     749    1533 
   Size:        122      63     156      63     103     242     784

  Fragments arranged by size: 

                 784     242     156     122     103      63      63
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     277     280     361     365    1533 
   Size:        277       3      81       4    1168

  Fragments arranged by size: 

                1168     277      81       4       3
 
BseYI C'CCAG_C

Cuts at:      0     234     918    1126    1395    1533 
   Size:        234     684     208     269     138

  Fragments arranged by size: 

                 684     269     234     208     138
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211    1533 
   Size:        211    1322
 
BsiEI CG_ry'CG

Cuts at:      0     174    1533 
   Size:        174    1359
 
BsiHKAI G_wGCw'C

Cuts at:      0    1142    1277    1533 
   Size:       1142     135     256

  Fragments arranged by size: 

                1142     256     135
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     712    1533 
   Size:        712     821
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     308    1113    1142    1254    1277    1298    1533 
   Size:        119     189     805      29     112      23      21     235

  Fragments arranged by size: 

                 805     235     189     119     112      29      23      21
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1492    1533 
   Size:       1492      41
 
BsrBI CCG'CTC

Cuts at:      0      68     255     887    1533 
   Size:         68     187     632     646

  Fragments arranged by size: 

                 646     632     187      68
 
BsrDI GCAATG_nn'

Cuts at:      0     690    1533 
   Size:        690     843
 
BsrFI r'CCGG_y

Cuts at:      0     488    1533 
   Size:        488    1045
 
BssSI C'ACGA_G

Cuts at:      0     634    1533 
   Size:        634     899
 
BstEII G'GTnAC_C

Cuts at:      0    1185    1533 
   Size:       1185     348
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     462    1533 
   Size:        462    1071
 
BstYI r'GATC_y

Cuts at:      0    1399    1533 
   Size:       1399     134
 
BtgI C'CryG_G

Cuts at:      0    1181    1337    1381    1533 
   Size:       1181     156      44     152

  Fragments arranged by size: 

                1181     156     152      44
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1266    1533 
   Size:       1266     267
 
BtsI GCAGTG_nn'

Cuts at:      0     228    1533 
   Size:        228    1305
 
DraIII CAC_nnn'GTG

Cuts at:      0     984    1533 
   Size:        984     549
 
EaeI y'GGCC_r

Cuts at:      0     486     851    1384    1533 
   Size:        486     365     533     149

  Fragments arranged by size: 

                 533     486     365     149
 
EarI CTCTTCn'nnn_

Cuts at:      0     541     595    1533 
   Size:        541      54     938

  Fragments arranged by size: 

                 938     541      54
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     127    1533 
   Size:        127    1406
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     646     892    1533 
   Size:        466     180     246     641

  Fragments arranged by size: 

                 641     466     246     180
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1517    1533 
   Size:       1517      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     543     996    1533 
   Size:        543     453     537

  Fragments arranged by size: 

                 543     537     453
 
FseI GG_CCGG'CC

Cuts at:      0     492    1533 
   Size:        492    1041
 
FspI TGC'GCA

Cuts at:      0      79     949     958    1533 
   Size:         79     870       9     575

  Fragments arranged by size: 

                 870     575      79       9
 
FspAI rTGC'GCAy

Cuts at:      0     949    1533 
   Size:        949     584
 
HaeII r_GCGC'y

Cuts at:      0     374     392     462     469     510     535     642    1133 
   Size:        374      18      70       7      41      25     107     491

Cuts at:   1133    1533 
   Size:        400

  Fragments arranged by size: 

                 491     400     374     107      70      41      25      18
                   7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     535     567    1533 
   Size:        535      32     966

  Fragments arranged by size: 

                 966     535      32
 
KasI G'GCGC_C

Cuts at:      0     506    1533 
   Size:        506    1027
 
MscI TGG'CCA

Cuts at:      0     853    1533 
   Size:        853     680
 
MslI CAynn'nnrTG

Cuts at:      0     694    1243    1285    1291    1533 
   Size:        694     549      42       6     242

  Fragments arranged by size: 

                 694     549     242      42       6
 
MspA1I CmG'CkG

Cuts at:      0     512    1383    1395    1467    1533 
   Size:        512     871      12      72      66

  Fragments arranged by size: 

                 871     512      72      66      12
 
NaeI GCC'GGC

Cuts at:      0     490    1533 
   Size:        490    1043
 
NarI GG'CG_CC

Cuts at:      0     507    1533 
   Size:        507    1026
 
NcoI C'CATG_G

Cuts at:      0    1337    1533 
   Size:       1337     196
 
NgoMIV G'CCGG_C

Cuts at:      0     488    1533 
   Size:        488    1045
 
NheI G'CTAG_C

Cuts at:      0    1018    1533 
   Size:       1018     515
 
NspI r_CATG'y

Cuts at:      0    1218    1242    1463    1527    1533 
   Size:       1218      24     221      64       6

  Fragments arranged by size: 

                1218     221      64      24       6
 
PasI CC'CwG_GG

Cuts at:      0    1200    1533 
   Size:       1200     333
 
PciI A'CATG_T

Cuts at:      0    1214    1459    1533 
   Size:       1214     245      74

  Fragments arranged by size: 

                1214     245      74
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     984    1533 
   Size:        984     549
 
PpuMI rG'GwC_Cy

Cuts at:      0     543     996    1533 
   Size:        543     453     537

  Fragments arranged by size: 

                 543     537     453
 
PshAI GACnn'nnGTC

Cuts at:      0    1460    1533 
   Size:       1460      73
 
PvuII CAG'CTG

Cuts at:      0    1395    1533 
   Size:       1395     138
 
SacII CC_GC'GG

Cuts at:      0    1384    1533 
   Size:       1384     149
 
SapI GCTCTTCn'nnn_

Cuts at:      0     541    1533 
   Size:        541     992
 
ScaI AGT'ACT

Cuts at:      0    1446    1533 
   Size:       1446      87
 
SfoI GGC'GCC

Cuts at:      0     508    1533 
   Size:        508    1025
 
SphI G_CATG'C

Cuts at:      0    1242    1533 
   Size:       1242     291
 
StuI AGG'CCT

Cuts at:      0     727    1533 
   Size:        727     806
 
StyI C'CwwG_G

Cuts at:      0       6     899     908    1058    1337    1533 
   Size:          6     893       9     150     279     196

  Fragments arranged by size: 

                 893     279     196     150       9       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     310    1533 
   Size:        310    1223
 
TatI w'GTAC_w

Cuts at:      0    1444    1533 
   Size:       1444      89
 
Tth111I GACn'n_nGTC

Cuts at:      0    1230    1533 
   Size:       1230     303
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     621     915    1197    1533 
   Size:        621     294     282     336

  Fragments arranged by size: 

                 621     336     294     282
 
XmnI GAAnn'nnTTC

Cuts at:      0    1312    1533 
   Size:       1312     221
 

 Enzymes that do cut:

     AclI     AfeI   AflIII     AleI     AlfI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI     BcgI     BclI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BpmI   Bpu10I     BsaI    BsaHI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FseI
     FspI    FspAI    HaeII    Hin4I     KasI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NspI     PasI     PciI
    PflMI    PpuMI    PshAI    PvuII    SacII     SapI     ScaI     SfoI
     SphI     StuI     StyI    TaqII     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI    AflII     AgeI     AhdI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI    BciVI    BfrBI    BglII     BmrI     BplI
    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BsiWI     BsmI    BspEI
    BspHI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FalI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NdeI
     NotI     NruI     NsiI     PacI     PfoI     PmeI     PmlI     PpiI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
     SalI    SanDI     SbfI    SexAI     SfcI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI     TstI
     XbaI     XhoI     XmaI     ZraI
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