Restriction map for pF1KB4161
 (Linear) (Six-base) MAPSORT of: KIBB4161.seq  Check: 5892  from: 1  to: 1413



KIBB4161.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     867    1413 
   Size:        867     546
 
AccI GT'mk_AC

Cuts at:      0     214    1413 
   Size:        214    1199
 
AflII C'TTAA_G

Cuts at:      0    1186    1413 
   Size:       1186     227
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      46      78    1413 
   Size:         46      32    1335

  Fragments arranged by size: 

                1335      46      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     492    1069    1413 
   Size:        291     201     577     344

  Fragments arranged by size: 

                 577     344     291     201
 
ApaI G_GGCC'C

Cuts at:      0     567     979    1413 
   Size:        567     412     434

  Fragments arranged by size: 

                 567     434     412
 
ApoI r'AATT_y

Cuts at:      0     340    1006    1413 
   Size:        340     666     407

  Fragments arranged by size: 

                 666     407     340
 
AvaI C'yCGr_G

Cuts at:      0     572    1142    1268    1292    1413 
   Size:        572     570     126      24     121

  Fragments arranged by size: 

                 572     570     126     121      24
 
BanI G'GyrC_C

Cuts at:      0    1231    1310    1406    1413 
   Size:       1231      79      96       7

  Fragments arranged by size: 

                1231      96      79       7
 
BanII G_rGCy'C

Cuts at:      0     567     979    1246    1413 
   Size:        567     412     267     167

  Fragments arranged by size: 

                 567     412     267     167
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1189    1413 
   Size:       1189     224
 
BbvCI CC'TCA_GC

Cuts at:      0     383    1413 
   Size:        383    1030
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     790    1413 
   Size:        790     623
 
Bme1580I G_kGCm'C

Cuts at:      0     567     979    1413 
   Size:        567     412     434

  Fragments arranged by size: 

                 567     434     412
 
BmgBI CAC'GTC

Cuts at:      0     468    1413 
   Size:        468     945
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     650    1413 
   Size:        650     763
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1091    1413 
   Size:       1091     322
 
Bpu10I CC'TnA_GC

Cuts at:      0     383    1413 
   Size:        383    1030
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     732    1413 
   Size:        732     681
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     446     476    1413 
   Size:        446      30     937

  Fragments arranged by size: 

                 937     446      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55     146     735     755     758     761     800     812 
   Size:         55      91     589      20       3       3      39      12

Cuts at:    812    1170    1413 
   Size:        358     243

  Fragments arranged by size: 

                 589     358     243      91      55      39      20      12
                   3       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1100    1413 
   Size:       1100     313
 
BsiHKAI G_wGCw'C

Cuts at:      0    1246    1413 
   Size:       1246     167
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1099    1413 
   Size:       1099     314
 
Bsp1286I G_dGCh'C

Cuts at:      0     567     979    1246    1413 
   Size:        567     412     267     167

  Fragments arranged by size: 

                 567     412     267     167
 
BspHI T'CATG_A

Cuts at:      0    1019    1413 
   Size:       1019     394
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     867    1413 
   Size:        867     546
 
BsrDI GCAATG_nn'

Cuts at:      0    1121    1413 
   Size:       1121     292
 
BsrFI r'CCGG_y

Cuts at:      0     474    1286    1403    1413 
   Size:        474     812     117      10

  Fragments arranged by size: 

                 812     474     117      10
 
BssSI C'ACGA_G

Cuts at:      0     638    1413 
   Size:        638     775
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     798    1413 
   Size:        798     615
 
BstEII G'GTnAC_C

Cuts at:      0    1277    1413 
   Size:       1277     136
 
BstYI r'GATC_y

Cuts at:      0    1317    1413 
   Size:       1317      96
 
BstZ17I GTA'TAC

Cuts at:      0     215    1413 
   Size:        215    1198
 
BtgI C'CryG_G

Cuts at:      0     559     957    1413 
   Size:        559     398     456

  Fragments arranged by size: 

                 559     456     398
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1246    1413 
   Size:       1246     167
 
BtsI GCAGTG_nn'

Cuts at:      0    1053    1413 
   Size:       1053     360
 
EaeI y'GGCC_r

Cuts at:      0     363     476    1413 
   Size:        363     113     937

  Fragments arranged by size: 

                 937     363     113
 
EarI CTCTTCn'nnn_

Cuts at:      0    1251    1413 
   Size:       1251     162
 
EcoICRI GAG'CTC

Cuts at:      0    1244    1413 
   Size:       1244     169
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1091    1413 
   Size:       1091     322
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     759     910     976    1413 
   Size:         54     705     151      66     437

  Fragments arranged by size: 

                 705     437     151      66      54
 
EcoRV GAT'ATC

Cuts at:      0    1017    1413 
   Size:       1017     396
 
FseI GG_CCGG'CC

Cuts at:      0     478    1413 
   Size:        478     935
 
HindIII A'AGCT_T

Cuts at:      0     750    1413 
   Size:        750     663
 
MfeI C'AATT_G

Cuts at:      0    1100    1413 
   Size:       1100     313
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     404    1413 
   Size:        404    1009
 
MscI TGG'CCA

Cuts at:      0     365    1413 
   Size:        365    1048
 
MspA1I CmG'CkG

Cuts at:      0     291    1413 
   Size:        291    1122
 
NaeI GCC'GGC

Cuts at:      0     476    1413 
   Size:        476     937
 
NcoI C'CATG_G

Cuts at:      0     957    1413 
   Size:        957     456
 
NgoMIV G'CCGG_C

Cuts at:      0     474    1413 
   Size:        474     939
 
PasI CC'CwG_GG

Cuts at:      0     844    1413 
   Size:        844     569
 
PfoI T'CCnGG_A

Cuts at:      0    1249    1413 
   Size:       1249     164
 
PpuMI rG'GwC_Cy

Cuts at:      0      54     759    1413 
   Size:         54     705     654

  Fragments arranged by size: 

                 705     654      54
 
PspOMI G'GGCC_C

Cuts at:      0     563     975    1413 
   Size:        563     412     438

  Fragments arranged by size: 

                 563     438     412
 
PvuII CAG'CTG

Cuts at:      0     291    1413 
   Size:        291    1122
 
SacI G_AGCT'C

Cuts at:      0    1246    1413 
   Size:       1246     167
 
SanDI GG'GwC_CC

Cuts at:      0     759    1413 
   Size:        759     654
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1251    1413 
   Size:       1251     162
 
SfcI C'TryA_G

Cuts at:      0     219     311     902     989    1221    1413 
   Size:        219      92     591      87     232     192

  Fragments arranged by size: 

                 591     232     219     192      92      87
 
SmaI CCC'GGG

Cuts at:      0    1144    1270    1294    1413 
   Size:       1144     126      24     119

  Fragments arranged by size: 

                1144     126     119      24
 
SmlI C'TyrA_G

Cuts at:      0     747    1186    1413 
   Size:        747     439     227

  Fragments arranged by size: 

                 747     439     227
 
StuI AGG'CCT

Cuts at:      0     937    1413 
   Size:        937     476
 
StyI C'CwwG_G

Cuts at:      0     957    1413 
   Size:        957     456
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1219    1413 
   Size:       1219     194
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     446     478    1413 
   Size:        446      32     935

  Fragments arranged by size: 

                 935     446      32
 
XmaI C'CCGG_G

Cuts at:      0    1142    1268    1292    1413 
   Size:       1142     126      24     121

  Fragments arranged by size: 

                1142     126     121      24
 

 Enzymes that do cut:

     AarI     AccI    AflII     AloI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbsI    BbvCI    BciVI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI    BsaXI    BseRI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BssSI   BstAPI   BstEII
    BstYI  BstZ17I     BtgI    BtgZI     BtsI     EaeI     EarI  EcoICRI
  Eco57MI EcoO109I    EcoRV     FseI  HindIII     MfeI     MmeI     MscI
   MspA1I     NaeI     NcoI   NgoMIV     PasI     PfoI    PpuMI   PspOMI
    PvuII     SacI    SanDI     SapI     SfcI     SmaI     SmlI     StuI
     StyI    TaqII     TstI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI    BsiWI     BsmI    BspEI    BsrBI    BsrGI   BssHII    BstBI
    BstXI   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI    EcoNI    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MluI     MslI     NarI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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