Restriction map for pF1KB4123
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BclI        BcnI        BfaI        BfrI        BlnI        
BsaAI       BsaHI       BseAI       BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CfoI        Cfr10I      ClaI        CpoI        DraI        
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
FauI        FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KspI        MaeI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NaeI        NarI        NciI        
NgoMIV      NheI        NotI        NruI        NspI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          880  GTAGAC               GT^[AC][GT]AC
AcsI         1111  AAATTT               [GA]^AATT[CT]
AflIII        659  ACACGT               A^C[GA][CT]GT
AocI         1315  CCTGAGG              CC^T[ACGT]AGG
ApaI         1294  GGGCCC               GGGCC^C
ApoI         1111  AAATTT               [GA]^AATT[CT]
AspHI         732  GTGCTC               G[AT]GC[AT]^C
BcgI         1005  CGATATATCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         496  GTATCC               GTATCC(6/5)
BglI           23  GCCTGTCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         575  AGATCT               A^GATCT
BlpI          507  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      507  GCTGAGC              GC^T[ACGT]AGC
BsaBI         914  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          867  GAGACC               GGTCTC(1/5)
BseRI         579  CTCCTC               GAGGAG(10/8)
BsgI         1369  GTGCAG               GTGCAG(16/14)
BsiHKAI       732  GTGCTC               G[AT]GC[AT]^C
BsiYI        1227  CCTCAAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1227  CCTCAAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         116  GTCCC                GGGAC(10/14)
Bsp1407I      401  TGTACA               T^GTACA
BspMI         512  GCAGGT               ACCTGC(4/8)
BsrGI         401  TGTACA               T^GTACA
BstAPI       1341  GCATGATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         590  TTCGAA               TT^CGAA
BstYI         575  AGATCT               [GA]^GATC[CT]
Bsu36I       1315  CCTGAGG              CC^T[ACGT]AGG
CelII         507  GCTGAGC              GC^T[ACGT]AGC
DraII        1309  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII        337  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          872  CCATGG               C^C[GA][CT]GG
Eco81I       1315  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1227  CCTCAAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1309  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I       888  ATGCAT               ATGCA^T
HgaI            7  GCGTC                GACGC(5/10)
MamI          914  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          575  AGATCT               [GA]^GATC[CT]
NcoI          872  CCATGG               C^CATGG
NsiI          888  ATGCAT               ATGCA^T
NspV          590  TTCGAA               TT^CGAA
SapI          318  GCTCTTC              GCTCTTC(1/4)
SfcI          312  CTACAG               C^T[GA][CT]AG
SfuI          590  TTCGAA               TT^CGAA
SmlI          802  CTCAAG               C^T[CT][GA]AG
SspBI         401  TGTACA               T^GTACA
StuI          927  AGGCCT               AGG^CCT
XhoII         575  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

Acc65I       1211  GGTACC               G^GTACC
             1352  GGTACC              
Asp718I      1211  GGTACC               G^GTACC
             1352  GGTACC              
AvaI          137  CTCGGG               C^[CT]CG[GA]G
              194  CTCGGG              
AvaII         654  GGTCC                G^G[AT]CC
              726  GGTCC               
BbsI          636  GAAGAC               GAAGAC(2/6)
             1390  GAAGAC              
BpmI           18  CTCCAG               CTGGAG(16/14)
             1046  CTGGAG              
BpuAI         636  GAAGAC               GAAGAC(2/6)
             1390  GAAGAC              
BsaWI         728  TCCGGT               [AT]^CCGG[AT]
             1208  TCCGGT              
BsmI         1266  GAATGC               GAATGC(1/-1)
              890  GCATTC              
BsoBI         137  CTCGGG               C^[CT]CG[GA]G
              194  CTCGGG              
BspHI         369  TCATGA               T^CATGA
             1262  TCATGA              
BsrI          677  ACTGG                ACTGG(1/-1)
             1045  ACTGG               
BssKI         265  CCAGG                ^CC[ACGT]GG
              585  CCTGG               
BstNI         265  CCAGG                CC^[AT]GG
              585  CCTGG               
EcoRII        265  CCAGG                ^CC[AT]GG
              585  CCTGG               
EcoT14I       720  CCAAGG               C^C[AT][AT]GG
              872  CCATGG              
KpnI         1211  GGTACC               GGTAC^C
             1352  GGTACC              
MspA1I        256  CAGCGG               C[AC]G^C[GT]G
              563  CAGCGG              
MvaI          265  CCAGG                CC^[AT]GG
              585  CCTGG               
NdeI          885  CATATG               CA^TATG
             1094  CATATG              
PleI          361  GAGTC                GAGTC(4/5)
              938  GAGTC               
RcaI          369  TCATGA               T^CATGA
             1262  TCATGA              
ScrFI         265  CCAGG                CC^[ACGT]GG
              585  CCTGG               
SspI          161  AATATT               AAT^ATT
              686  AATATT              
StyI          720  CCAAGG               C^C[AT][AT]GG
              872  CCATGG              
TaqI          364  TCGA                 T^CGA
              591  TCGA                
TfiI          125  GAATC                G^A[AT]TC
              366  GATTC               
TthHB8I       364  TCGA                 T^CGA
              591  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BanI        
BanII       BbvI        BmyI        BsaJI       BsmAI       
Bsp1286I    BstF5I      Cac8I       Cfr13I      DdeI        
DpnII       EarI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      SfaNI       Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com