Restriction map for pF1KB4091
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BclI        BcnI        BfrI        
BglI        BlnI        BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BspDI       BspEI       BspHI       BsrBI       
BsrFI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       Bsu36I      
Cac8I       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MfeI        
MluI        MroI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         1261  GTATAC               GT^[AC][GT]AC
AciI         1407  GCGG                 CCGC(-3/-1)
AflIII       1040  ACATGT               A^C[GA][CT]GT
Aor51HI         5  AGCGCT               AGC^GCT
AspHI         542  GAGCTC               G[AT]GC[AT]^C
AvaI          323  CCCGAG               C^[CT]CG[GA]G
BalI         1107  TGGCCA               TGG^CCA
BanI          790  GGCACC               G^G[CT][GA]CC
BcgI          904  CGAAGACATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          179  CTAG                 C^TAG
BglII         405  AGATCT               A^GATCT
BlpI           36  GCTCAGC              GC^T[ACGT]AGC
BpmI         1011  CTCCAG               CTGGAG(16/14)
Bpu1102I       36  GCTCAGC              GC^T[ACGT]AGC
BsaBI          79  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        1432  GAGGAG               GAGGAG(10/8)
BsiHKAI       542  GAGCTC               G[AT]GC[AT]^C
BsoBI         323  CCCGAG               C^[CT]CG[GA]G
BspLU11I     1040  ACATGT               A^CATGT
BspMI         223  GCAGGT               ACCTGC(4/8)
Bst1107I     1261  GTATAC               GTA^TAC
BstF5I       1269  GGATG                GGATG(2/0)
BstZ17I      1261  GTATAC               GTA^TAC
CelII          36  GCTCAGC              GC^T[ACGT]AGC
CfoI            6  GCGC                 GCG^C
DraII         225  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DsaI          640  CCGTGG               C^C[GA][CT]GG
Eco47III        5  AGCGCT               AGC^GCT
EcoO109I      225  AGGTCCC              [GA]G^G[ACGT]CC[CT]
FokI         1269  GGATG                GGATG(9/13)
HaeII           5  AGCGCT               [GA]GCGC^[CT]
HhaI            6  GCGC                 GCG^C
HinP1I          6  GCGC                 G^CGC
MaeI          179  CTAG                 C^TAG
MaeII         582  ACGT                 A^CGT
MamI           79  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1107  TGGCCA               TGG^CCA
MscI         1107  TGGCCA               TGG^CCA
MslI         1035  CATGGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        617  CAGCTG               C[AC]G^C[GT]G
NdeI          839  CATATG               CA^TATG
NspI         1040  ACATGT               [GA]CATG^[CT]
PpuMI         225  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          620  CTGCAG               CTGCA^G
PvuII         617  CAGCTG               CAG^CTG
SacI          542  GAGCTC               GAGCT^C
XcmI          794  CCAATGAAGACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BanII          34  GGGCTC               G[GA]GC[CT]^C
              542  GAGCTC              
BbsI          799  GAAGAC               GAAGAC(2/6)
              905  GAAGAC              
BciVI         291  GGATAC               GTATCC(6/5)
              567  GTATCC              
BpuAI         799  GAAGAC               GAAGAC(2/6)
              905  GAAGAC              
BsgI          231  CTGCAC               GTGCAG(16/14)
             1425  CTGCAC              
BsiYI          52  CCCCAGTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1385  CCTGGTACGGG         
BslI           52  CCCCAGTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1385  CCTGGTACGGG         
BsmI          364  GAATGC               GAATGC(1/-1)
             1092  GAATGC              
BstYI         405  AGATCT               [GA]^GATC[CT]
             1140  GGATCT              
EaeI          637  TGGCCG               [CT]^GGCC[GA]
             1107  TGGCCA              
HphI           67  GGTGA                GGTGA(8/7)
              537  TCACC               
MflI          405  AGATCT               [GA]^GATC[CT]
             1140  GGATCT              
MseI          332  TTAA                 T^TAA
              587  TTAA                
PleI          554  GACTC                GAGTC(4/5)
              884  GACTC               
SfcI          620  CTGCAG               C^T[GA][CT]AG
              747  CTACAG              
TaqI          903  TCGA                 T^CGA
             1340  TCGA                
Tru9I         332  TTAA                 T^TAA
              587  TTAA                
Tsp45I       1122  GTCAC                ^GT[GC]AC
             1162  GTGAC               
TthHB8I       903  TCGA                 T^CGA
             1340  TCGA                
XhoII         405  AGATCT               [GA]^GATC[CT]
             1140  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AvaII       BbvI        BmyI        BsaJI       BsmFI       
Bsp1286I    Bsp1407I    BsrDI       BsrGI       BsrI        
BssKI       BstNI       Cfr13I      DdeI        DpnII       
EcoRII      EcoT14I     Fnu4HI      HaeIII      HinfI       
ItaI        MaeIII      MboI        MboII       MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SspBI       StyI        TfiI        TseI        Tsp509I     
TspRI       
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