Restriction map for pF1KB4091
 (Linear) (Six-base) MAPSORT of: KIBB4091.seq  Check: 4886  from: 1  to: 1440



KIBB4091.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:      0    1262    1440 
   Size:       1262     178
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203    1005    1440 
   Size:        203     802     435

  Fragments arranged by size: 

                 802     435     203
 
AfeI AGC'GCT

Cuts at:      0       7    1440 
   Size:          7    1433
 
AflIII A'CryG_T

Cuts at:      0    1040    1440 
   Size:       1040     400
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     246    1440 
   Size:        246    1194
 
ApoI r'AATT_y

Cuts at:      0     442     654    1305    1440 
   Size:        442     212     651     135

  Fragments arranged by size: 

                 651     442     212     135
 
AvaI C'yCGr_G

Cuts at:      0     323    1440 
   Size:        323    1117
 
BanI G'GyrC_C

Cuts at:      0     790    1440 
   Size:        790     650
 
BanII G_rGCy'C

Cuts at:      0      38     546    1440 
   Size:         38     508     894

  Fragments arranged by size: 

                 894     508      38
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     806     912    1440 
   Size:        806     106     528

  Fragments arranged by size: 

                 806     528     106
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     893     927    1440 
   Size:        893      34     513

  Fragments arranged by size: 

                 893     513      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     285     578    1440 
   Size:        285     293     862

  Fragments arranged by size: 

                 862     293     285
 
BglII A'GATC_T

Cuts at:      0     405    1440 
   Size:        405    1035
 
BlpI GC'TnA_GC

Cuts at:      0      37    1440 
   Size:         37    1403
 
Bme1580I G_kGCm'C

Cuts at:      0     436    1440 
   Size:        436    1004
 
BmgBI CAC'GTC

Cuts at:      0     583    1440 
   Size:        583     857
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      48     493    1440 
   Size:         48     445     947

  Fragments arranged by size: 

                 947     445      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     996    1440 
   Size:        996     444
 
Bpu10I CC'TnA_GC

Cuts at:      0     474    1440 
   Size:        474     966
 
BsaBI GATnn'nnATC

Cuts at:      0      83    1440 
   Size:         83    1357
 
BseYI C'CCAG_C

Cuts at:      0    1419    1440 
   Size:       1419      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     216    1410    1440 
   Size:        216    1194      30

  Fragments arranged by size: 

                1194     216      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     546    1440 
   Size:        546     894
 
BsmI GAATG_Cn'

Cuts at:      0     370    1098    1440 
   Size:        370     728     342

  Fragments arranged by size: 

                 728     370     342
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     436     546    1440 
   Size:         38     398     110     894

  Fragments arranged by size: 

                 894     398     110      38
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     214    1440 
   Size:        214    1226
 
BsrDI GCAATG_nn'

Cuts at:      0     779     909    1245    1402    1440 
   Size:        779     130     336     157      38

  Fragments arranged by size: 

                 779     336     157     130      38
 
BsrGI T'GTAC_A

Cuts at:      0     400     776    1203    1440 
   Size:        400     376     427     237

  Fragments arranged by size: 

                 427     400     376     237
 
BstYI r'GATC_y

Cuts at:      0     405    1140    1440 
   Size:        405     735     300

  Fragments arranged by size: 

                 735     405     300
 
BstZ17I GTA'TAC

Cuts at:      0    1263    1440 
   Size:       1263     177
 
BtgI C'CryG_G

Cuts at:      0     640    1440 
   Size:        640     800
 
BtsI GCAGTG_nn'

Cuts at:      0    1004    1440 
   Size:       1004     436
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     372     407    1440 
   Size:        372      35    1033

  Fragments arranged by size: 

                1033     372      35
 
EaeI y'GGCC_r

Cuts at:      0     637    1107    1440 
   Size:        637     470     333

  Fragments arranged by size: 

                 637     470     333
 
EcoICRI GAG'CTC

Cuts at:      0     544    1440 
   Size:        544     896
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     996    1005    1440 
   Size:        203     793       9     435

  Fragments arranged by size: 

                 793     435     203       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     226    1440 
   Size:        226    1214
 
HaeII r_GCGC'y

Cuts at:      0       9    1440 
   Size:          9    1431
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     181     213     330     362    1440 
   Size:        181      32     117      32    1078

  Fragments arranged by size: 

                1078     181     117      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1177    1440 
   Size:       1177     263
 
MscI TGG'CCA

Cuts at:      0    1109    1440 
   Size:       1109     331
 
MslI CAynn'nnrTG

Cuts at:      0    1039    1440 
   Size:       1039     401
 
MspA1I CmG'CkG

Cuts at:      0     619    1440 
   Size:        619     821
 
NdeI CA'TA_TG

Cuts at:      0     840    1440 
   Size:        840     600
 
NspI r_CATG'y

Cuts at:      0    1044    1440 
   Size:       1044     396
 
PciI A'CATG_T

Cuts at:      0    1040    1440 
   Size:       1040     400
 
PpuMI rG'GwC_Cy

Cuts at:      0     226    1440 
   Size:        226    1214
 
PsiI TTA'TAA

Cuts at:      0     604    1440 
   Size:        604     836
 
PstI C_TGCA'G

Cuts at:      0     624    1440 
   Size:        624     816
 
PvuII CAG'CTG

Cuts at:      0     619    1440 
   Size:        619     821
 
SacI G_AGCT'C

Cuts at:      0     546    1440 
   Size:        546     894
 
SfcI C'TryA_G

Cuts at:      0     620     747    1440 
   Size:        620     127     693

  Fragments arranged by size: 

                 693     620     127
 
StyI C'CwwG_G

Cuts at:      0     693    1057    1378    1440 
   Size:        693     364     321      62

  Fragments arranged by size: 

                 693     364     321      62
 
TatI w'GTAC_w

Cuts at:      0     400     776    1203    1440 
   Size:        400     376     427     237

  Fragments arranged by size: 

                 427     400     376     237
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     225     257    1440 
   Size:        225      32    1183

  Fragments arranged by size: 

                1183     225      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     801    1440 
   Size:        801     639
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AlfI     ApoI     AvaI     BanI
    BanII     BbsI     BcgI    BciVI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BsaBI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrDI    BsrGI    BstYI  BstZ17I     BtgI     BtsI
    CspCI     EaeI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I     MmeI
     MscI     MslI   MspA1I     NdeI     NspI     PciI    PpuMI     PsiI
     PstI    PvuII     SacI     SfcI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BclI    BfrBI     BglI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI   Bsu36I    BtgZI     ClaI
     DraI   DraIII     DrdI     EagI     EarI     EciI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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