Restriction map for pF1KB4086
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BcgI        BciVI       BfrI        BglI        
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsoBI       Bsp1286I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
BstUI       Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FseI        FspI        HaeII       HhaI        HinP1I      
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspI        
StuI        StyI        SwaI        Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcyI         1403  GGCGTC               G[GA]^CG[CT]C
Asp700I       840  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI           37  TGGCCA               TGG^CCA
BanI         1254  GGCACC               G^G[CT][GA]CC
BbvCI        1084  CCTCAGC              CCTCAGC(-5/-2)
BclI           93  TGATCA               T^GATCA
BcnI         1180  CCGGG                CC^[GC]GG
BglII          68  AGATCT               A^GATCT
BsaHI        1403  GGCGTC               G[GA]^CG[CT]C
BsgI          174  CTGCAC               GTGCAG(16/14)
BsmAI         283  GAGAC                GTCTC(1/5)
Bsp1407I     1031  TGTACA               T^GTACA
BspHI         779  TCATGA               T^CATGA
BsrGI        1031  TGTACA               T^GTACA
Bst1107I      138  GTATAC               GTA^TAC
BstXI         143  CCATTGGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       138  GTATAC               GTA^TAC
EaeI           37  TGGCCA               [CT]^GGCC[GA]
EcoT22I       380  ATGCAT               ATGCA^T
FbaI           93  TGATCA               T^GATCA
HapII        1180  CCGG                 C^CGG
HgaI         1404  GCGTC                GACGC(5/10)
Hin1I        1403  GGCGTC               G[GA]^CG[CT]C
HindIII        29  AAGCTT               A^AGCTT
HpaI          418  GTTAAC               GTT^AAC
HpaII        1180  CCGG                 C^CGG
MaeII        1354  ACGT                 A^CGT
MaeIII       1162  GTCAC                ^GT[ACGT]AC
MluNI          37  TGGCCA               TGG^CCA
MscI           37  TGGCCA               TGG^CCA
MspI         1180  CCGG                 C^CGG
NciI         1180  CCGGG                CC^[GC]GG
NdeI          589  CATATG               CA^TATG
NheI          964  GCTAGC               G^CTAGC
NsiI          380  ATGCAT               ATGCA^T
RcaI          779  TCATGA               T^CATGA
ScaI         1076  AGTACT               AGT^ACT
SmlI           64  CTTGAG               C^T[CT][GA]AG
SphI          382  GCATGC               GCATG^C
SspBI        1031  TGTACA               T^GTACA
Tsp45I       1162  GTCAC                ^GT[GC]AC
XmnI          840  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI          138  GTATAC               GT^[AC][GT]AC
              703  GTCTAC              
AciI          690  CCGC                 CCGC(-3/-1)
              264  GCGG                
AlwI          717  GATCC                GGATC(4/5)
                7  GGATC               
AlwNI         398  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1523  CAGAACCTG           
BsmFI         583  GGGAC                GGGAC(10/14)
             1406  GTCCC               
BsmI           21  GAATGC               GAATGC(1/-1)
             1452  GCATTC              
BstYI          68  AGATCT               [GA]^GATC[CT]
              716  AGATCC              
Cfr13I        153  GGGCC                G^G[ACGT]CC
              544  GGGCC               
HincII        418  GTTAAC               GT[CT]^[GA]AC
             1309  GTCAAC              
HindII        418  GTTAAC               GT[CT]^[GA]AC
             1309  GTCAAC              
HphI          793  TCACC                GGTGA(8/7)
             1081  TCACC               
MflI           68  AGATCT               [GA]^GATC[CT]
              716  AGATCC              
MslI          207  CATGACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              598  CATGCCTATG          
NlaIV        1051  GGTTCC               GG[ACGT]^[ACGT]CC
             1254  GGCACC              
NspI          382  GCATGC               [GA]CATG^[CT]
              597  ACATGC              
PflMI         434  CCAGCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1299  CCATCATTTGG         
Sau96I        153  GGGCC                G^G[ACGT]CC
              544  GGGCC               
TaqI          293  TCGA                 T^CGA
              368  TCGA                
TthHB8I       293  TCGA                 T^CGA
              368  TCGA                
Van91I        434  CCAGCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1299  CCATCATTTGG         
XhoII          68  AGATCT               [GA]^GATC[CT]
              716  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AfaI        AluI        BbsI        BbvI        BfaI        
BpuAI       BsaJI       BsiYI       BslI        BsrI        
BssKI       BstF5I      BstNI       Cac8I       DdeI        
DpnII       EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        Ksp632I     MaeI        
MboI        MboII       MnlI        MseI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      PleI        
PvuII       RsaI        Sau3AI      ScrFI       SfaNI       
SfcI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       
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