Restriction map for pF1KB4086
 (Linear) (Six-base) MAPSORT of: KIBB4086.seq  Check: 6359  from: 1  to: 1560



KIBB4086.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:      0     139     704    1560 
   Size:        139     565     856

  Fragments arranged by size: 

                 856     565     139
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     963    1025    1550    1560 
   Size:        963      62     525      10

  Fragments arranged by size: 

                 963     525      62      10
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      48    1560 
   Size:         48    1512
 
AlwNI CAG_nnn'CTG

Cuts at:      0     403    1528    1560 
   Size:        403    1125      32

  Fragments arranged by size: 

                1125     403      32
 
BanI G'GyrC_C

Cuts at:      0    1254    1560 
   Size:       1254     306
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     319     399     926    1257    1560 
   Size:        319      80     527     331     303

  Fragments arranged by size: 

                 527     331     319     303      80
 
BbvCI CC'TCA_GC

Cuts at:      0    1085    1560 
   Size:       1085     475
 
BclI T'GATC_A

Cuts at:      0      93    1560 
   Size:         93    1467
 
BfrBI ATG'CAT

Cuts at:      0     382    1560 
   Size:        382    1178
 
BglII A'GATC_T

Cuts at:      0      68    1560 
   Size:         68    1492
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     720    1560 
   Size:        720     840
 
BmtI G_CTAG'C

Cuts at:      0     968    1560 
   Size:        968     592
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     453    1560 
   Size:        453    1107
 
Bpu10I CC'TnA_GC

Cuts at:      0    1085    1560 
   Size:       1085     475
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85    1560 
   Size:         85    1475
 
BsaHI Gr'CG_yC

Cuts at:      0    1404    1560 
   Size:       1404     156
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1490    1520    1560 
   Size:       1490      30      40

  Fragments arranged by size: 

                1490      40      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159    1560 
   Size:        159    1401
 
BsmI GAATG_Cn'

Cuts at:      0      27    1452    1560 
   Size:         27    1425     108

  Fragments arranged by size: 

                1425     108      27
 
BspHI T'CATG_A

Cuts at:      0     779    1560 
   Size:        779     781
 
BsrGI T'GTAC_A

Cuts at:      0    1031    1560 
   Size:       1031     529
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     150    1560 
   Size:        150    1410
 
BstYI r'GATC_y

Cuts at:      0      68     716    1560 
   Size:         68     648     844

  Fragments arranged by size: 

                 844     648      68
 
BstZ17I GTA'TAC

Cuts at:      0     140    1560 
   Size:        140    1420
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      29      64    1560 
   Size:         29      35    1496

  Fragments arranged by size: 

                1496      35      29
 
EaeI y'GGCC_r

Cuts at:      0      37    1560 
   Size:         37    1523
 
EarI CTCTTCn'nnn_

Cuts at:      0     332     982    1096    1560 
   Size:        332     650     114     464

  Fragments arranged by size: 

                 650     464     332     114
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     963    1025    1550    1560 
   Size:        963      62     525      10

  Fragments arranged by size: 

                 963     525      62      10
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     186    1560 
   Size:        186    1374
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     181     213     613     645    1210    1242    1560 
   Size:        181      32     400      32     565      32     318

  Fragments arranged by size: 

                 565     400     318     181      32      32      32
 
HincII GTy'rAC

Cuts at:      0     420    1311    1560 
   Size:        420     891     249

  Fragments arranged by size: 

                 891     420     249
 
HindIII A'AGCT_T

Cuts at:      0      29    1560 
   Size:         29    1531
 
HpaI GTT'AAC

Cuts at:      0     420    1560 
   Size:        420    1140
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     255     501    1560 
   Size:         86     169     246    1059

  Fragments arranged by size: 

                1059     246     169      86
 
MscI TGG'CCA

Cuts at:      0      39    1560 
   Size:         39    1521
 
MslI CAynn'nnrTG

Cuts at:      0     211     602    1560 
   Size:        211     391     958

  Fragments arranged by size: 

                 958     391     211
 
MspA1I CmG'CkG

Cuts at:      0     330     400     692    1537    1560 
   Size:        330      70     292     845      23

  Fragments arranged by size: 

                 845     330     292      70      23
 
NdeI CA'TA_TG

Cuts at:      0     590    1560 
   Size:        590     970
 
NheI G'CTAG_C

Cuts at:      0     964    1560 
   Size:        964     596
 
NsiI A_TGCA'T

Cuts at:      0     384    1560 
   Size:        384    1176
 
NspI r_CATG'y

Cuts at:      0     386     601    1560 
   Size:        386     215     959

  Fragments arranged by size: 

                 959     386     215
 
PasI CC'CwG_GG

Cuts at:      0    1323    1560 
   Size:       1323     237
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     440    1305    1560 
   Size:        440     865     255

  Fragments arranged by size: 

                 865     440     255
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1383    1415    1560 
   Size:       1383      32     145

  Fragments arranged by size: 

                1383     145      32
 
PvuII CAG'CTG

Cuts at:      0     330     400    1537    1560 
   Size:        330      70    1137      23

  Fragments arranged by size: 

                1137     330      70      23
 
ScaI AGT'ACT

Cuts at:      0    1078    1560 
   Size:       1078     482
 
SfcI C'TryA_G

Cuts at:      0     509     641     882    1560 
   Size:        509     132     241     678

  Fragments arranged by size: 

                 678     509     241     132
 
SmlI C'TyrA_G

Cuts at:      0      64    1560 
   Size:         64    1496
 
SphI G_CATG'C

Cuts at:      0     386    1560 
   Size:        386    1174
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1312    1560 
   Size:       1312     248
 
TatI w'GTAC_w

Cuts at:      0    1031    1076    1148    1560 
   Size:       1031      45      72     412

  Fragments arranged by size: 

                1031     412      72      45
 
XmnI GAAnn'nnTTC

Cuts at:      0     844    1560 
   Size:        844     716
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     BanI     BbsI    BbvCI     BclI
    BfrBI    BglII     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaHI
    BsaXI     BsgI     BsmI    BspHI    BsrGI    BstXI    BstYI  BstZ17I
    CspCI     EaeI     EarI  Eco57MI     FalI    Hin4I   HincII  HindIII
     HpaI     MmeI     MscI     MslI   MspA1I     NdeI     NheI     NsiI
     NspI     PasI    PflMI     PpiI    PvuII     ScaI     SfcI     SmlI
     SphI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BcgI
    BciVI     BglI     BlpI Bme1580I    BmgBI     BpmI     BsaI    BsaAI
    BsaBI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NotI     NruI
     PacI     PciI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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