Restriction map for pF1KB4080
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        ApaI        ApaLI       AscI        AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BcgI        BclI        BcnI        BfrI        BglI        
BlnI        BpmI        BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
BstUI       Bsu36I      Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HpaI        KasI        Ksp632I     KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

Acc65I        613  GGTACC               G^GTACC
AccI          674  GTATAC               GT^[AC][GT]AC
AciI          801  GCGG                 CCGC(-3/-1)
AlwI          483  GGATC                GGATC(4/5)
Aor51HI       302  AGCGCT               AGC^GCT
AseI          589  ATTAAT               AT^TAAT
AsnI          589  ATTAAT               AT^TAAT
Asp700I       765  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       613  GGTACC               G^GTACC
AvaI          839  CTCGAG               C^[CT]CG[GA]G
BanI          613  GGTACC               G^G[CT][GA]CC
BanII         317  GAGCCC               G[GA]GC[CT]^C
BbsI          474  GAAGAC               GAAGAC(2/6)
BciVI         150  GGATAC               GTATCC(6/5)
BglII         956  AGATCT               A^GATCT
BlpI          556  GCTCAGC              GC^T[ACGT]AGC
BmyI          317  GAGCCC               G[AGT]GC[ACT]^C
Bpu1102I      556  GCTCAGC              GC^T[ACGT]AGC
BpuAI         474  GAAGAC               GAAGAC(2/6)
BsgI          427  CTGCAC               GTGCAG(16/14)
BsiYI         896  CCAAAGACGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          896  CCAAAGACGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         869  GAGAC                GTCTC(1/5)
BsmI          644  GCATTC               GAATGC(1/-1)
BsoBI         839  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      317  GAGCCC               G[AGT]GC[ACT]^C
BspLU11I      137  ACATGT               A^CATGT
BssKI         944  CCTGG                ^CC[ACGT]GG
Bst1107I      674  GTATAC               GTA^TAC
BstNI         944  CCTGG                CC^[AT]GG
BstYI         956  AGATCT               [GA]^GATC[CT]
BstZ17I       674  GTATAC               GTA^TAC
Cac8I         791  GCTTGC               GC[ACGT]^[ACGT]GC
CelII         556  GCTCAGC              GC^T[ACGT]AGC
CfoI          303  GCGC                 GCG^C
Eco47III      302  AGCGCT               AGC^GCT
EcoRII        944  CCTGG                ^CC[AT]GG
EcoRV         184  GATATC               GAT^ATC
HaeII         302  AGCGCT               [GA]GCGC^[CT]
HaeIII        363  GGCC                 GG^CC
HapII         148  CCGG                 C^CGG
HhaI          303  GCGC                 GCG^C
HinP1I        303  GCGC                 G^CGC
HindIII       195  AAGCTT               A^AGCTT
HpaII         148  CCGG                 C^CGG
HphI          287  TCACC                GGTGA(8/7)
KpnI          613  GGTACC               GGTAC^C
MaeII          85  ACGT                 A^CGT
MaeIII        344  GTTAC                ^GT[ACGT]AC
MflI          956  AGATCT               [GA]^GATC[CT]
MslI          366  CATCCCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          148  CCGG                 C^CGG
MvaI          944  CCTGG                CC^[AT]GG
PaeR7I        839  CTCGAG               C^TCGAG
PleI          871  GACTC                GAGTC(4/5)
PshBI         589  ATTAAT               AT^TAAT
PvuII         927  CAGCTG               CAG^CTG
ScaI          995  AGTACT               AGT^ACT
ScrFI         944  CCTGG                CC^[ACGT]GG
XhoI          839  CTCGAG               C^TCGAG
XhoII         956  AGATCT               [GA]^GATC[CT]
XmnI          765  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AflIII         83  ACACGT               A^C[GA][CT]GT
              137  ACATGT              
BbvI          300  GCAGC                GCAGC(8/12)
              721  GCTGC               
BfaI          523  CTAG                 C^TAG
              713  CTAG                
BstXI         376  CCACCACACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              774  CCAAATTCATGG        
DpnII         484  GATC                 ^GATC
              957  GATC                
EcoRI         503  GAATTC               G^AATTC
              512  GAATTC              
MaeI          523  CTAG                 C^TAG
              713  CTAG                
MboI          484  GATC                 ^GATC
              957  GATC                
MspA1I        799  CAGCGG               C[AC]G^C[GT]G
              927  CAGCTG              
MwoI          712  GCTAGAGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              795  GCAACAGCGGC         
NdeII         484  GATC                 ^GATC
              957  GATC                
NspI          137  ACATGT               [GA]CATG^[CT]
              881  ACATGC              
Sau3AI        484  GATC                 ^GATC
              957  GATC                
TaqI          188  TCGA                 T^CGA
              840  TCGA                
TfiI           12  GAATC                G^A[AT]TC
              282  GAATC               
TseI          300  GCAGC                G^C[AT]GC
              721  GCTGC               
TthHB8I       188  TCGA                 T^CGA
              840  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BsrI        
BstF5I      DdeI        Fnu4HI      FokI        HinfI       
ItaI        MboII       MnlI        MseI        NlaIII      
NlaIV       RsaI        SfaNI       SmlI        Tru9I       
Tsp509I     TspRI       
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