Restriction map for
pF1KB4055
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BpmI BsaAI
BsaBI BsaI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I Cfr10I CpoI DraI
DsaI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT14I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI TfiI Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 542 GACGTC GACGT^C
AcyI 542 GACGTC G[GA]^CG[CT]C
AhdI 582 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 558 AGCT AG^CT
Asp700I 572 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 582 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 889 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 738 GGTCC G^G[AT]CC
BanI 935 GGCACC G^G[CT][GA]CC
BbsI 895 GTCTTC GAAGAC(2/6)
BlpI 804 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 804 GCTCAGC GC^T[ACGT]AGC
BpuAI 895 GTCTTC GAAGAC(2/6)
BsaHI 542 GACGTC G[GA]^CG[CT]C
BsaJI 660 CCTCGG C^C[ACGT][ACGT]GG
BsaWI 755 ACCGGA [AT]^CCGG[AT]
Bsp1407I 944 TGTACA T^GTACA
BspDI 78 ATCGAT AT^CGAT
BspHI 482 TCATGA T^CATGA
BsrGI 944 TGTACA T^GTACA
CelII 804 GCTCAGC GC^T[ACGT]AGC
ClaI 78 ATCGAT AT^CGAT
DraII 428 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 937 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 792 GACCACGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 582 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 130 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 428 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 319 GATATC GAT^ATC
EcoT22I 702 ATGCAT ATGCA^T
HaeII 634 AGCGCC [GA]GCGC^[CT]
HgaI 893 GCGTC GACGC(5/10)
Hin1I 542 GACGTC G[GA]^CG[CT]C
HphI 329 TCACC GGTGA(8/7)
MaeII 543 ACGT A^CGT
MaeIII 860 GTGAC ^GT[ACGT]AC
MseI 272 TTAA T^TAA
MslI 729 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 702 ATGCAT ATGCA^T
PflFI 889 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 482 TCATGA T^CATGA
ScaI 29 AGTACT AGT^ACT
SspBI 944 TGTACA T^GTACA
SspI 221 AATATT AAT^ATT
Tru9I 272 TTAA T^TAA
Tsp45I 860 GTGAC ^GT[GC]AC
Tth111I 889 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 369 TCTAGA T^CTAGA
XmnI 572 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BalI 210 TGGCCA TGG^CCA
815 TGGCCA
BanII 853 GAGCCC G[GA]GC[CT]^C
972 GAGCCC
BbvI 783 GCAGC GCAGC(8/12)
830 GCAGC
BcnI 939 CCCGG CC^[GC]GG
975 CCCGG
BseRI 292 CTCCTC GAGGAG(10/8)
759 GAGGAG
BsmAI 164 GAGAC GTCTC(1/5)
762 GAGAC
BstAPI 502 GCAAGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
685 GCAGGGATTGC
BstYI 476 AGATCC [GA]^GATC[CT]
531 AGATCC
CfoI 635 GCGC GCG^C
828 GCGC
Cfr13I 429 GGGCC G^G[ACGT]CC
738 GGTCC
EaeI 210 TGGCCA [CT]^GGCC[GA]
815 TGGCCA
EcoRI 105 GAATTC G^AATTC
346 GAATTC
HhaI 635 GCGC GCG^C
828 GCGC
HinP1I 635 GCGC G^CGC
828 GCGC
HinfI 16 GACTC G^A[ACGT]TC
196 GACTC
MflI 476 AGATCC [GA]^GATC[CT]
531 AGATCC
MluNI 210 TGGCCA TGG^CCA
815 TGGCCA
MscI 210 TGGCCA TGG^CCA
815 TGGCCA
MspA1I 831 CAGCGG C[AC]G^C[GT]G
921 CAGCGG
NciI 939 CCCGG CC^[GC]GG
975 CCCGG
NlaIII 471 CATG CATG^
483 CATG
NlaIV 664 GGTTCC GG[ACGT]^[ACGT]CC
935 GGCACC
PleI 16 GACTC GAGTC(4/5)
196 GACTC
Sau96I 429 GGGCC G^G[ACGT]CC
738 GGTCC
TseI 783 GCAGC G^C[AT]GC
830 GCAGC
TspRI 812 CACTG CA[GC]TG[ACGT][ACGT]^
858 CAGTG
XhoII 476 AGATCC [GA]^GATC[CT]
531 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AfaI AlwI ApoI
BfaI BmyI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII ItaI
MaeI MboI MboII MnlI MspI
MvaI MwoI NdeII RsaI Sau3AI
ScrFI SfaNI TaqI Tsp509I TthHB8I