Restriction map for pF1KB4038
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstUI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MfeI        MluI        MroI        MseI        
MunI        MvnI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI          628  GTCTAC               GT^[AC][GT]AC
AcsI          204  AAATTT               [GA]^AATT[CT]
AlwNI         877  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           43  CCTGAGG              CC^T[ACGT]AGG
ApaI          241  GGGCCC               GGGCC^C
ApoI          204  AAATTT               [GA]^AATT[CT]
AspI          669  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          892  GGTGCC               G^G[CT][GA]CC
BbvCI         658  GCTGAGG              CCTCAGC(-5/-2)
BsaI          667  GAGACC               GGTCTC(1/5)
BsaWI        1082  TCCGGT               [AT]^CCGG[AT]
BsgI          542  CTGCAC               GTGCAG(16/14)
BspHI         302  TCATGA               T^CATGA
BsrFI        1049  GCCGGC               [GA]^CCGG[CT]
BssSI         437  CTCGTG               CACGAG(-5/-1)
BstEII       1002  GGTAACC              G^GT[ACGT]ACC
BstPI        1002  GGTAACC              G^GT[ACGT]ACC
Bsu36I         43  CCTGAGG              CC^T[ACGT]AGG
CfoI         1026  GCGC                 GCG^C
Cfr10I       1049  GCCGGC               [GA]^CCGG[CT]
DrdI          691  GACAGCTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I         43  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1002  GGTAACC              G^GT[ACGT]ACC
EcoT14I       399  CCTTGG               C^C[AT][AT]GG
FauI          245  CCCGC                CCCGC(4/6)
HhaI         1026  GCGC                 GCG^C
HinP1I       1026  GCGC                 G^CGC
HincII        799  GTCAAC               GT[CT]^[GA]AC
HindII        799  GTCAAC               GT[CT]^[GA]AC
MslI          303  CATGAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        769  CAGCTG               C[AC]G^C[GT]G
NaeI         1049  GCCGGC               GCC^GGC
NgoMIV       1049  GCCGGC               G^CCGGC
NspI         1074  GCATGT               [GA]CATG^[CT]
PflFI         669  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         876  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          50  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         769  CAGCTG               CAG^CTG
RcaI          302  TCATGA               T^CATGA
SacI          298  GAGCTC               GAGCT^C
SapI          379  GAAGAGC              GCTCTTC(1/4)
SexAI         881  ACCTGGT              A^CC[AT]GGT
SfaNI          75  GATGC                GCATC(5/9)
SfcI          278  CTACAG               C^T[GA][CT]AG
SmlI          324  CTTGAG               C^T[CT][GA]AG
StyI          399  CCTTGG               C^C[AT][AT]GG
TfiI          582  GAATC                G^A[AT]TC
Tsp45I        844  GTGAC                ^GT[GC]AC
Tsp509I       205  AATT                 ^AATT
Tth111I       669  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        876  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          228  CCAAGTAGGGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AciI          246  CCGC                 CCGC(-3/-1)
              765  GCGG                
AlwI          612  GGATC                GGATC(4/5)
              861  GGATC               
AspHI         298  GAGCTC               G[AT]GC[AT]^C
              388  GTGCTC              
BbsI          898  GAAGAC               GAAGAC(2/6)
             1078  GTCTTC              
BcnI          369  CCGGG                CC^[GC]GG
              848  CCCGG               
BglII         779  AGATCT               A^GATCT
              950  AGATCT              
BpuAI         898  GAAGAC               GAAGAC(2/6)
             1078  GTCTTC              
BsiHKAI       298  GAGCTC               G[AT]GC[AT]^C
              388  GTGCTC              
BsmAI         667  GAGAC                GTCTC(1/5)
              606  GTCTC               
BsrI           20  ACTGG                ACTGG(1/-1)
              672  CCAGT               
BstYI         779  AGATCT               [GA]^GATC[CT]
              950  AGATCT              
EarI          534  CTCTTC               CTCTTC(1/4)
              379  GAAGAG              
Ksp632I       534  CTCTTC               CTCTTC(1/4)
              379  GAAGAG              
MaeIII        844  GTGAC                ^GT[ACGT]AC
             1003  GTAAC               
MflI          779  AGATCT               [GA]^GATC[CT]
              950  AGATCT              
NciI          369  CCGGG                CC^[GC]GG
              848  CCCGG               
TaqI          223  TCGA                 T^CGA
              273  TCGA                
TspRI         761  CAGTG                CA[GC]TG[ACGT][ACGT]^
              888  CAGTG               
TthHB8I       223  TCGA                 T^CGA
              273  TCGA                
XhoII         779  AGATCT               [GA]^GATC[CT]
              950  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AfaI        AluI        AvaII       BalI        BanII       
BbvI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmFI       Bsp1286I    BspMI       
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MboI        
MboII       MluNI       MnlI        MscI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
StuI        TseI        
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