Restriction map for
pF1KB4038
(Circular) (Six-base) MAPSORT of: pF1KB4038.seq Check: 94 from: 1 to: 4202
pF1KB4038 4202 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 737 1240 737
Size: 503 3699
Acc65I G'GTAC_C
Cuts at: 1218 1218
Size: 4202
AclI AA'CG_TT
Cuts at: 4042 4042
Size: 4202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2459 3206 3474 2027
Size: 432 747 268 2755
Fragments arranged by size:
2755 747 432 268
AflIII A'CryG_T
Cuts at: 1554 2658 3635 1554
Size: 1104 977 2121
Fragments arranged by size:
2121 1104 977
AgeI A'CCGG_T
Cuts at: 2590 2590
Size: 4202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 138 138
Size: 4202
AlwNI CAG_nnn'CTG
Cuts at: 990 1507 3074 990
Size: 517 1567 2118
Fragments arranged by size:
2118 1567 517
ApaI G_GGCC'C
Cuts at: 353 353
Size: 4202
ApaLI G'TGCA_C
Cuts at: 2972 2972
Size: 4202
ApoI r'AATT_y
Cuts at: 312 1206 3555 3811 312
Size: 894 2349 256 703
Fragments arranged by size:
2349 894 703 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4202
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
AvaI C'yCGr_G
Cuts at: 1222 1222
Size: 4202
BamHI G'GATC_C
Cuts at: 1227 1227
Size: 4202
BanI G'GyrC_C
Cuts at: 1000 1218 1632 1880 1915 1000
Size: 218 414 248 35 3287
Fragments arranged by size:
3287 414 248 218 35
BanII G_rGCy'C
Cuts at: 264 353 410 1216 2246 264
Size: 89 57 806 1030 2220
Fragments arranged by size:
2220 1030 806 89 57
BbeI G_GCGC'C
Cuts at: 1636 1884 1636
Size: 248 3954
BbsI GAAGACnn'nnnn_
Cuts at: 1013 1179 1013
Size: 166 4036
BbvCI CC'TCA_GC
Cuts at: 767 767
Size: 4202
BciVI GTATCCnnnnn_n'
Cuts at: 2100 2861 4202 2100
Size: 761 1341 2100
Fragments arranged by size:
2100 1341 761
BclI T'GATC_A
Cuts at: 1722 1722
Size: 4202
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4202
BglII A'GATC_T
Cuts at: 887 1058 1717 3754 887
Size: 171 659 2037 1335
Fragments arranged by size:
2037 1335 659 171
BlpI GC'TnA_GC
Cuts at: 1314 1314
Size: 4202
Bme1580I G_kGCm'C
Cuts at: 353 1827 1920 2976 353
Size: 1474 93 1056 1579
Fragments arranged by size:
1579 1474 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 774 1414 1495 1830 3974 774
Size: 640 81 335 2144 1002
Fragments arranged by size:
2144 1002 640 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 424 2569 424
Size: 2145 2057
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 763 868 967 1114 591
Size: 172 105 99 147 3679
Fragments arranged by size:
3679 172 147 105 99
Bpu10I CC'TnA_GC
Cuts at: 575 767 575
Size: 192 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 1378 2257 2749 3047 453
Size: 925 879 492 298 1608
Fragments arranged by size:
1608 925 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 769 3906 769
Size: 3137 1065
BsaAI yAC'GTr
Cuts at: 1555 1555
Size: 4202
BsaBI GATnn'nnATC
Cuts at: 6 1716 3753 6
Size: 1710 2037 455
Fragments arranged by size:
2037 1710 455
BsaHI Gr'CG_yC
Cuts at: 1633 1881 2583 4079 1633
Size: 248 702 1496 1756
Fragments arranged by size:
1756 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1190 1391 1912 2590 2864 3011 3422 1190
Size: 201 521 678 274 147 411 1970
Fragments arranged by size:
1970 678 521 411 274 201 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 620 650 1036 1066 620
Size: 30 386 30 3756
Fragments arranged by size:
3756 386 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 418 1057 169
Size: 249 639 3314
Fragments arranged by size:
3314 639 249
BseYI C'CCAG_C
Cuts at: 596 672 1627 2385 2962 3458 596
Size: 76 955 758 577 496 1340
Fragments arranged by size:
1340 955 758 577 496 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 635
Size: 4202
BsiEI CG_ry'CG
Cuts at: 104 1744 1790 2998 104
Size: 1640 46 1208 1308
Fragments arranged by size:
1640 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 410 500 1216 1994 2184 2976 410
Size: 90 716 778 190 792 1636
Fragments arranged by size:
1636 792 778 716 190 90
Bsp1286I G_dGCh'C
Cuts at: 264 353 410 500 1216 1827 1920 1994 2184
Size: 89 57 90 716 611 93 74 190
Cuts at: 2184 2246 2976 264
Size: 62 730 1490
Fragments arranged by size:
1490 730 716 611 190 93 90 89
74 62 57
BspEI T'CCGG_A
Cuts at: 3422 3422
Size: 4202
BspHI T'CATG_A
Cuts at: 410 3378 410
Size: 2968 1234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 127 301 771 1252 1466 1768 2149 127
Size: 174 470 481 214 302 381 2180
Fragments arranged by size:
2180 481 470 381 302 214 174
BsrBI CCG'CTC
Cuts at: 50 2494 2548 4032 4197 50
Size: 2444 54 1484 165 55
Fragments arranged by size:
2444 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2114 2114
Size: 4202
BsrFI r'CCGG_y
Cuts at: 1157 2200 2381 2590 1157
Size: 1043 181 209 2769
Fragments arranged by size:
2769 1043 209 181
BssSI C'ACGA_G
Cuts at: 545 2473 2831 545
Size: 1928 358 1916
Fragments arranged by size:
1928 1916 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1579 1579
Size: 4202
BstBI TT'CG_AA
Cuts at: 2563 2563
Size: 4202
BstEII G'GTnAC_C
Cuts at: 1110 1110
Size: 4202
BstYI r'GATC_y
Cuts at: 1 887 1058 1227 1694 1717 2052 2298 3310
Size: 886 171 169 467 23 335 246 1012
Cuts at: 3310 3396 3408 3754 1
Size: 86 12 346 449
Fragments arranged by size:
1012 886 467 449 346 335 246 171
169 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 152 152
Size: 4202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2152 2333 2496 2152
Size: 181 163 3858
Fragments arranged by size:
3858 181 163
BtsI GCAGTG_nn'
Cuts at: 1445 1445
Size: 4202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 237 272 237
Size: 35 4167
DraI TTT'AAA
Cuts at: 1201 1201
Size: 4202
DrdI GACnn_nn'nnGTC
Cuts at: 805 1908 2766 805
Size: 1103 858 2241
Fragments arranged by size:
2241 1103 858
EaeI y'GGCC_r
Cuts at: 278 510 705 752 1101 1787 1961 2352 2379
Size: 232 195 47 349 686 174 391 27
Cuts at: 2379 3724 278
Size: 1345 756
Fragments arranged by size:
1345 756 686 391 349 232 195 174
47 27
EagI C'GGCC_G
Cuts at: 1787 1787
Size: 4202
EarI CTCTTCn'nnn_
Cuts at: 482 648 2225 2435 482
Size: 166 1577 210 2249
Fragments arranged by size:
2249 1577 210 166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2410 2720 2866 4012 2410
Size: 310 146 1146 2600
Fragments arranged by size:
2600 1146 310 146
EcoICRI GAG'CTC
Cuts at: 408 1214 408
Size: 806 3396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 763 868 967 1114 2027 2459 3206 3474
Size: 172 105 99 147 913 432 747 268
Cuts at: 3474 591
Size: 1319
Fragments arranged by size:
1319 913 747 432 268 172 147 105
99
EcoO109I rG'GnC_Cy
Cuts at: 159 350 821 1341 159
Size: 191 471 520 3020
Fragments arranged by size:
3020 520 471 191
EcoRI G'AATT_C
Cuts at: 1206 1206
Size: 4202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 4202
FspI TGC'GCA
Cuts at: 1983 1983
Size: 4202
HaeII r_GCGC'y
Cuts at: 1636 1884 2906 3885 1636
Size: 248 1022 979 1953
Fragments arranged by size:
1953 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 426 458 585 617 766 798 879 911 2044
Size: 32 127 32 149 32 81 32 1133
Cuts at: 2044 2074 2076 2106 426
Size: 30 2 30 2522
Fragments arranged by size:
2522 1133 149 127 81 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 909 1241 909
Size: 332 3870
KasI G'GCGC_C
Cuts at: 1632 1880 1632
Size: 248 3954
KpnI G_GTAC'C
Cuts at: 1222 1222
Size: 4202
MluI A'CGCG_T
Cuts at: 3635 3635
Size: 4202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 221 2873 3057 221
Size: 2652 184 1366
Fragments arranged by size:
2652 1366 184
MscI TGG'CCA
Cuts at: 512 707 754 1103 1963 512
Size: 195 47 349 860 2751
Fragments arranged by size:
2751 860 349 195 47
MslI CAynn'nnrTG
Cuts at: 415 2191 2318 415
Size: 1776 127 2299
Fragments arranged by size:
2299 1776 127
MspA1I CmG'CkG
Cuts at: 879 1313 1627 1987 3000 3245 879
Size: 434 314 360 1013 245 1836
Fragments arranged by size:
1836 1013 434 360 314 245
NaeI GCC'GGC
Cuts at: 1159 2383 1159
Size: 1224 2978
NarI GG'CG_CC
Cuts at: 1633 1881 1633
Size: 248 3954
NgoMIV G'CCGG_C
Cuts at: 1157 2381 1157
Size: 1224 2978
NspI r_CATG'y
Cuts at: 1186 1255 1755 2662 1186
Size: 69 500 907 2726
Fragments arranged by size:
2726 907 500 69
PasI CC'CwG_GG
Cuts at: 171 202 171
Size: 31 4171
PciI A'CATG_T
Cuts at: 2658 2658
Size: 4202
PflMI CCAn_nnn'nTGG
Cuts at: 990 990
Size: 4202
PmeI GTTT'AAAC
Cuts at: 1201 1201
Size: 4202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 4170
PpuMI rG'GwC_Cy
Cuts at: 159 159
Size: 4202
PspOMI G'GGCC_C
Cuts at: 349 349
Size: 4202
PstI C_TGCA'G
Cuts at: 1249 1934 1249
Size: 685 3517
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4202
PvuII CAG'CTG
Cuts at: 879 1627 1987 879
Size: 748 360 3094
Fragments arranged by size:
3094 748 360
RsrII CG'GwC_CG
Cuts at: 1193 2397 1193
Size: 1204 2998
SacI G_AGCT'C
Cuts at: 410 1216 410
Size: 806 3396
SalI G'TCGA_C
Cuts at: 1239 1239
Size: 4202
SapI GCTCTTCn'nnn_
Cuts at: 482 2225 2435 482
Size: 1743 210 2249
Fragments arranged by size:
2249 1743 210
SbfI CC_TGCA'GG
Cuts at: 1249 1249
Size: 4202
ScaI AGT'ACT
Cuts at: 3719 3719
Size: 4202
SexAI A'CCwGG_T
Cuts at: 989 989
Size: 4202
SfcI C'TryA_G
Cuts at: 33 386 1245 1930 2923 3114 33
Size: 353 859 685 993 191 1121
Fragments arranged by size:
1121 993 859 685 353 191
SfoI GGC'GCC
Cuts at: 1634 1882 1634
Size: 248 3954
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
SmaI CCC'GGG
Cuts at: 1224 1224
Size: 4202
SmlI C'TyrA_G
Cuts at: 432 1357 2272 2764 3026 432
Size: 925 915 492 262 1608
Fragments arranged by size:
1608 925 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4202
SphI G_CATG'C
Cuts at: 1255 1755 1255
Size: 500 3702
StuI AGG'CCT
Cuts at: 190 586 863 190
Size: 396 277 3529
Fragments arranged by size:
3529 396 277
StyI C'CwwG_G
Cuts at: 507 1336 1690 507
Size: 829 354 3019
Fragments arranged by size:
3019 829 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 645 1089 1785 1918 2377 2586 645
Size: 444 696 133 459 209 2261
Fragments arranged by size:
2261 696 459 444 209 133
TatI w'GTAC_w
Cuts at: 3717 3717
Size: 4202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 620 651 652 683 1036 1068 3719 3751 620
Size: 31 1 31 353 32 2651 32 1071
Fragments arranged by size:
2651 1071 353 32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 780 1999 780
Size: 1219 2983
XbaI T'CTAG_A
Cuts at: 1233 1233
Size: 4202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 343 1697 343
Size: 1354 2848
XmaI C'CCGG_G
Cuts at: 1222 1222
Size: 4202
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstXI BstZ17I BtgI ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI