Restriction map for pF1KB4028
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcyI        
AflII       AflIII      AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BanI        BanII       
BbeI        BbrPI       BbvCI       BclI        BfrI        
BglI        BglII       BlnI        BmyI        BsaAI       
BsaHI       BsaI        BseAI       BsiEI       BsiHKAI     
BsiWI       BsoBI       Bsp1286I    Bsp1407I    BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     CpoI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRV       FbaI        
FseI        FspI        HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MluI        MluNI       MroI        MscI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp45I      Tth111I     XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AclI          734  AACGTT               AA^CGTT
AfaI          835  GTAC                 GT^AC
AgeI          143  ACCGGT               A^CCGGT
AocI          702  CCTCAGG              CC^T[ACGT]AGG
AvaII          82  GGTCC                G^G[AT]CC
BamHI         707  GGATCC               G^GATCC
BbsI          871  GAAGAC               GAAGAC(2/6)
BcgI          177  GCAGCCAATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         1584  CCGGG                CC^[GC]GG
BlpI         1596  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1596  GCTGAGC              GC^T[ACGT]AGC
BpuAI         871  GAAGAC               GAAGAC(2/6)
BsaBI        1360  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         143  ACCGGT               [AT]^CCGG[AT]
BsgI          634  CTGCAC               GTGCAG(16/14)
BsmBI        1348  GAGACG               CGTCTC(1/5)
BsmFI        1252  GTCCC                GGGAC(10/14)
BspDI        1268  ATCGAT               AT^CGAT
BsrFI         143  ACCGGT               [GA]^CCGG[CT]
BstYI         707  GGATCC               [GA]^GATC[CT]
Bsu36I        702  CCTCAGG              CC^T[ACGT]AGG
CelII        1596  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        143  ACCGGT               [GA]^CCGG[CT]
Cfr13I         82  GGTCC                G^G[ACGT]CC
ClaI         1268  ATCGAT               AT^CGAT
Eco81I        702  CCTCAGG              CC^T[ACGT]AGG
EcoT22I        89  ATGCAT               ATGCA^T
HaeII          15  GGCGCT               [GA]GCGC^[CT]
HaeIII       1582  GGCC                 GG^CC
HincII        157  GTTGAC               GT[CT]^[GA]AC
HindII        157  GTTGAC               GT[CT]^[GA]AC
MamI         1360  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          860  CAATTG               C^AATTG
MflI          707  GGATCC               [GA]^GATC[CT]
MslI          307  CATCTTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          860  CAATTG               C^AATTG
NciI         1584  CCGGG                CC^[GC]GG
NcoI         1233  CCATGG               C^CATGG
NdeI          416  CATATG               CA^TATG
NsiI           89  ATGCAT               ATGCA^T
PflMI        1315  CCAAAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         143  ACCGGT               A^CCGGT
Psp1406I      734  AACGTT               AA^CGTT
RsaI          835  GTAC                 GT^AC
Sau96I         82  GGTCC                G^G[ACGT]CC
Van91I       1315  CCAAAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1260  TCTAGA               T^CTAGA
XhoII         707  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         510  CGCG                 CG^CG
             1210  CGCG                
AlwNI          49  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              964  CAGGCTCTG           
BfaI          896  CTAG                 C^TAG
             1261  CTAG                
BseRI        1159  GAGGAG               GAGGAG(10/8)
             1162  GAGGAG              
BsmAI        1348  GAGAC                GTCTC(1/5)
             1290  GTCTC               
BsrDI         442  GCAATG               GCAATG(2/0)
             1486  GCAATG              
BstUI         510  CGCG                 CG^CG
             1210  CGCG                
CfoI           16  GCGC                 GCG^C
             1211  GCGC                
DsaI          593  CCGTGG               C^C[GA][CT]GG
             1233  CCATGG              
EcoRI        1310  GAATTC               G^AATTC
             1434  GAATTC              
EcoT14I       989  CCTTGG               C^C[AT][AT]GG
             1233  CCATGG              
FauI          162  CCCGC                CCCGC(4/6)
             1646  CCCGC               
HapII         144  CCGG                 C^CGG
             1584  CCGG                
HhaI           16  GCGC                 GCG^C
             1211  GCGC                
HinP1I         16  GCGC                 G^CGC
             1211  GCGC                
HpaII         144  CCGG                 C^CGG
             1584  CCGG                
MaeI          896  CTAG                 C^TAG
             1261  CTAG                
MspI          144  CCGG                 C^CGG
             1584  CCGG                
MvnI          510  CGCG                 CG^CG
             1210  CGCG                
NlaIII        867  CATG                 CATG^
             1234  CATG                
PvuII         817  CAGCTG               CAG^CTG
             1670  CAGCTG              
StyI          989  CCTTGG               C^C[AT][AT]GG
             1233  CCATGG              
TfiI         1360  GATTC                G^A[AT]TC
             1383  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
Asp700I     BbvI        BciVI       BpmI        BsaJI       
BsiYI       BslI        BsmI        BsrI        BssKI       
BstF5I      BstNI       Cac8I       DdeI        DpnII       
DraI        EcoRII      Fnu4HI      FokI        HinfI       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MvaI        
MwoI        NdeII       NlaIV       PleI        Sau3AI      
ScrFI       SfaNI       TaqI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XmnI        
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