Restriction map for pF1KB3998
##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaII       AviII       
AvrII       BalI        BamHI       BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaJI       BseRI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmI        Bsp1286I    Bsp1407I    BspDI       BspHI       
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MflI        MluI        MluNI       MscI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XcmI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccIII        537  TCCGGA               T^CCGGA
AciI          205  CCGC                 CCGC(-3/-1)
AcsI           79  AAATTT               [GA]^AATT[CT]
AfaI           97  GTAC                 GT^AC
AflIII         49  ACATGT               A^C[GA][CT]GT
AlwNI         416  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI           79  AAATTT               [GA]^AATT[CT]
AvaI          529  CTCGAG               C^[CT]CG[GA]G
BanI          291  GGTGCC               G^G[CT][GA]CC
BbvI          523  GCTGC                GCAGC(8/12)
BpmI          413  CTCCAG               CTGGAG(16/14)
BsaI          252  GGTCTC               GGTCTC(1/5)
BsaWI         537  TCCGGA               [AT]^CCGG[AT]
BseAI         537  TCCGGA               T^CCGGA
BsgI          473  CTGCAC               GTGCAG(16/14)
BsiEI         202  CGACCG               CG[GA][CT]^CG
BsmFI         340  GGGAC                GGGAC(10/14)
BsoBI         529  CTCGAG               C^[CT]CG[GA]G
BspEI         537  TCCGGA               T^CCGGA
BspLU11I       49  ACATGT               A^CATGT
Cac8I         432  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I        435  GGCCC                G^G[ACGT]CC
DpnII         352  GATC                 ^GATC
EcoRV          60  GATATC               GAT^ATC
Fnu4HI        523  GCTGC                GC^[ACGT]GC
HaeIII        435  GGCC                 GG^CC
HapII         538  CCGG                 C^CGG
HpaII         538  CCGG                 C^CGG
HphI          306  TCACC                GGTGA(8/7)
ItaI          523  GCTGC                GC^[ACGT]GC
MaeII         275  ACGT                 A^CGT
MboI          352  GATC                 ^GATC
MroI          537  TCCGGA               T^CCGGA
MseI           11  TTAA                 T^TAA
MslI          126  CACAGTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        205  CCGCTG               C[AC]G^C[GT]G
MspI          538  CCGG                 C^CGG
MwoI          514  GCTATCCTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         352  GATC                 ^GATC
NlaIII         50  CATG                 CATG^
NlaIV         291  GGTGCC               GG[ACGT]^[ACGT]CC
NspI           49  ACATGT               [GA]CATG^[CT]
PaeR7I        529  CTCGAG               C^TCGAG
PstI          173  CTGCAG               CTGCA^G
RsaI           97  GTAC                 GT^AC
Sau3AI        352  GATC                 ^GATC
Sau96I        435  GGCCC                G^G[ACGT]CC
SfcI          173  CTGCAG               C^T[GA][CT]AG
SmlI          529  CTCGAG               C^T[CT][GA]AG
TaqI          530  TCGA                 T^CGA
Tru9I          11  TTAA                 T^TAA
TseI          523  GCTGC                G^C[AT]GC
TthHB8I       530  TCGA                 T^CGA
XhoI          529  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AluI          431  AGCT                 AG^CT
              522  AGCT                
BfaI          235  CTAG                 C^TAG
              520  CTAG                
HinfI         321  GACTC                G^A[ACGT]TC
              342  GACTC               
MaeI          235  CTAG                 C^TAG
              520  CTAG                
MaeIII        124  GTCAC                ^GT[ACGT]AC
              440  GTGAC               
MboII         495  GAAGA                GAAGA(8/7)
              262  TCTTC               
PleI          321  GACTC                GAGTC(4/5)
              342  GACTC               
Tsp45I        124  GTCAC                ^GT[GC]AC
              440  GTGAC               
TspRI         248  CAGTG                CA[GC]TG[ACGT][ACGT]^
              310  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

BsmAI       DdeI        MnlI        Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com