Restriction map for pF1KB3906
 (Linear) (Six-base) MAPSORT of: KIBB3906.seq  Check: 3577  from: 1  to: 1254



KIBB3906.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81    1254 
   Size:         81    1173
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     372     769    1254 
   Size:        372     397     485

  Fragments arranged by size: 

                 485     397     372
 
ApoI r'AATT_y

Cuts at:      0     544    1254 
   Size:        544     710
 
AseI AT'TA_AT

Cuts at:      0    1085    1254 
   Size:       1085     169
 
AvaI C'yCGr_G

Cuts at:      0      53    1254 
   Size:         53    1201
 
BanI G'GyrC_C

Cuts at:      0      11      49      76    1254 
   Size:         11      38      27    1178

  Fragments arranged by size: 

                1178      38      27      11
 
BanII G_rGCy'C

Cuts at:      0     184     474    1254 
   Size:        184     290     780

  Fragments arranged by size: 

                 780     290     184
 
BbeI G_GCGC'C

Cuts at:      0      15      80    1254 
   Size:         15      65    1174

  Fragments arranged by size: 

                1174      65      15
 
BfrBI ATG'CAT

Cuts at:      0       3    1254 
   Size:          3    1251
 
BlpI GC'TnA_GC

Cuts at:      0     398    1254 
   Size:        398     856
 
Bme1580I G_kGCm'C

Cuts at:      0      54     200    1254 
   Size:         54     146    1054

  Fragments arranged by size: 

                1054     146      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     132     177    1254 
   Size:        132      45    1077

  Fragments arranged by size: 

                1077     132      45
 
BsaAI yAC'GTr

Cuts at:      0     630    1254 
   Size:        630     624
 
BsaHI Gr'CG_yC

Cuts at:      0      12      77    1254 
   Size:         12      65    1177

  Fragments arranged by size: 

                1177      65      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     286     694     937    1254 
   Size:        148     138     408     243     317

  Fragments arranged by size: 

                 408     317     243     148     138
 
BsiHKAI G_wGCw'C

Cuts at:      0     907    1254 
   Size:        907     347
 
BsiWI C'GTAC_G

Cuts at:      0     215    1254 
   Size:        215    1039
 
Bsp1286I G_dGCh'C

Cuts at:      0      54     184     200     474     907    1254 
   Size:         54     130      16     274     433     347

  Fragments arranged by size: 

                 433     347     274     130      54      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     170    1254 
   Size:        170    1084
 
BsrDI GCAATG_nn'

Cuts at:      0     644    1254 
   Size:        644     610
 
BsrGI T'GTAC_A

Cuts at:      0     440    1254 
   Size:        440     814
 
BtgI C'CryG_G

Cuts at:      0       7     227    1254 
   Size:          7     220    1027

  Fragments arranged by size: 

                1027     220       7
 
BtsI GCAGTG_nn'

Cuts at:      0     267     285    1189    1254 
   Size:        267      18     904      65

  Fragments arranged by size: 

                 904     267      65      18
 
DraIII CAC_nnn'GTG

Cuts at:      0     126    1254 
   Size:        126    1128
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     323    1254 
   Size:        323     931
 
EarI CTCTTCn'nnn_

Cuts at:      0     172     729    1254 
   Size:        172     557     525

  Fragments arranged by size: 

                 557     525     172
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81    1254 
   Size:         81    1173
 
EcoO109I rG'GnC_Cy

Cuts at:      0      92     223     463    1254 
   Size:         92     131     240     791

  Fragments arranged by size: 

                 791     240     131      92
 
EcoRV GAT'ATC

Cuts at:      0     144    1254 
   Size:        144    1110
 
HaeII r_GCGC'y

Cuts at:      0      15      80    1254 
   Size:         15      65    1174

  Fragments arranged by size: 

                1174      65      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1100    1132    1254 
   Size:       1100      32     122

  Fragments arranged by size: 

                1100     122      32
 
KasI G'GCGC_C

Cuts at:      0      11      76    1254 
   Size:         11      65    1178

  Fragments arranged by size: 

                1178      65      11
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     908    1254 
   Size:         89     819     346

  Fragments arranged by size: 

                 819     346      89
 
MslI CAynn'nnrTG

Cuts at:      0     751    1254 
   Size:        751     503
 
MspA1I CmG'CkG

Cuts at:      0       9     288    1201    1254 
   Size:          9     279     913      53

  Fragments arranged by size: 

                 913     279      53       9
 
NarI GG'CG_CC

Cuts at:      0      12      77    1254 
   Size:         12      65    1177

  Fragments arranged by size: 

                1177      65      12
 
NsiI A_TGCA'T

Cuts at:      0       5    1254 
   Size:          5    1249
 
NspI r_CATG'y

Cuts at:      0    1198    1254 
   Size:       1198      56
 
PpuMI rG'GwC_Cy

Cuts at:      0      92     463    1254 
   Size:         92     371     791

  Fragments arranged by size: 

                 791     371      92
 
PsiI TTA'TAA

Cuts at:      0    1157    1177    1254 
   Size:       1157      20      77

  Fragments arranged by size: 

                1157      77      20
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     593     625    1254 
   Size:        593      32     629

  Fragments arranged by size: 

                 629     593      32
 
PvuII CAG'CTG

Cuts at:      0     288    1201    1254 
   Size:        288     913      53

  Fragments arranged by size: 

                 913     288      53
 
SacII CC_GC'GG

Cuts at:      0      10    1254 
   Size:         10    1244
 
SapI GCTCTTCn'nnn_

Cuts at:      0     172    1254 
   Size:        172    1082
 
SfoI GGC'GCC

Cuts at:      0      13      78    1254 
   Size:         13      65    1176

  Fragments arranged by size: 

                1176      65      13
 
SmaI CCC'GGG

Cuts at:      0      55    1254 
   Size:         55    1199
 
SphI G_CATG'C

Cuts at:      0    1198    1254 
   Size:       1198      56
 
SspI AAT'ATT

Cuts at:      0     435    1254 
   Size:        435     819
 
StyI C'CwwG_G

Cuts at:      0     559    1062    1254 
   Size:        559     503     192

  Fragments arranged by size: 

                 559     503     192
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     114    1254 
   Size:        114    1140
 
TatI w'GTAC_w

Cuts at:      0     440     493    1184    1254 
   Size:        440      53     691      70

  Fragments arranged by size: 

                 691     440      70      53
 
XmaI C'CCGG_G

Cuts at:      0      53    1254 
   Size:         53    1201
 
XmnI GAAnn'nnTTC

Cuts at:      0     605    1076    1254 
   Size:        605     471     178

  Fragments arranged by size: 

                 605     471     178
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     AseI     AvaI     BanI    BanII     BbeI
    BfrBI     BlpI Bme1580I     BmrI    BsaAI    BsaHI     BsgI  BsiHKAI
    BsiWI Bsp1286I    BspMI    BsrDI    BsrGI     BtgI     BtsI   DraIII
     DrdI     EarI  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I     KasI
     MmeI     MslI   MspA1I     NarI     NsiI     NspI    PpuMI     PsiI
     PsrI    PvuII    SacII     SapI     SfoI     SmaI     SphI     SspI
     StyI    TaqII     TatI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PstI     PvuI
    RsrII     SacI     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI
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