Restriction map for pF1KB3799
 (Linear) (Six-base) MAPSORT of: KIBB3799.seq  Check: 968  from: 1  to: 1455



KIBB3799.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:      0    1160    1455 
   Size:       1160     295
 
AclI AA'CG_TT

Cuts at:      0      64    1455 
   Size:         64    1391
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1204    1455 
   Size:       1204     251
 
AfeI AGC'GCT

Cuts at:      0     146     193     780    1455 
   Size:        146      47     587     675

  Fragments arranged by size: 

                 675     587     146      47
 
AflIII A'CryG_T

Cuts at:      0      47     452    1289    1455 
   Size:         47     405     837     166

  Fragments arranged by size: 

                 837     405     166      47
 
AleI CACnn'nnGTG

Cuts at:      0     650     829    1455 
   Size:        650     179     626

  Fragments arranged by size: 

                 650     626     179
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     124     424    1455 
   Size:        124     300    1031

  Fragments arranged by size: 

                1031     300     124
 
AlwNI CAG_nnn'CTG

Cuts at:      0     111     162     183    1455 
   Size:        111      51      21    1272

  Fragments arranged by size: 

                1272     111      51      21
 
ApaI G_GGCC'C

Cuts at:      0     203     306     315    1455 
   Size:        203     103       9    1140

  Fragments arranged by size: 

                1140     203     103       9
 
ApaLI G'TGCA_C

Cuts at:      0    1297    1358    1455 
   Size:       1297      61      97

  Fragments arranged by size: 

                1297      97      61
 
AvaI C'yCGr_G

Cuts at:      0     299    1100    1455 
   Size:        299     801     355

  Fragments arranged by size: 

                 801     355     299
 
BamHI G'GATC_C

Cuts at:      0    1104    1455 
   Size:       1104     351
 
BanI G'GyrC_C

Cuts at:      0     134     320     408     606    1455 
   Size:        134     186      88     198     849

  Fragments arranged by size: 

                 849     198     186     134      88
 
BanII G_rGCy'C

Cuts at:      0     203     306     315     898    1057    1213    1455 
   Size:        203     103       9     583     159     156     242

  Fragments arranged by size: 

                 583     242     203     159     156     103       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     975    1455 
   Size:        975     480
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     225     259     454     488    1413    1447    1455 
   Size:        225      34     195      34     925      34       8

  Fragments arranged by size: 

                 925     225     195      34      34      34       8
 
BglI GCCn_nnn'nGGC

Cuts at:      0     310    1455 
   Size:        310    1145
 
Bme1580I G_kGCm'C

Cuts at:      0     137     203     306     315     323     413    1301    1362 
   Size:        137      66     103       9       8      90     888      61

Cuts at:   1362    1455 
   Size:         93

  Fragments arranged by size: 

                 888     137     103      93      90      66      61       9
                   8
 
BmgBI CAC'GTC

Cuts at:      0     416     455    1455 
   Size:        416      39    1000

  Fragments arranged by size: 

                1000     416      39
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1227    1455 
   Size:       1227     228
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     882    1392    1455 
   Size:        882     510      63

  Fragments arranged by size: 

                 882     510      63
 
BsaAI yAC'GTr

Cuts at:      0     510    1455 
   Size:        510     945
 
BsaHI Gr'CG_yC

Cuts at:      0    1415    1455 
   Size:       1415      40
 
BseYI C'CCAG_C

Cuts at:      0     130     141     183    1455 
   Size:        130      11      42    1272

  Fragments arranged by size: 

                1272     130      42      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     780    1455 
   Size:        780     675
 
BsiHKAI G_wGCw'C

Cuts at:      0     225    1213    1301    1362    1455 
   Size:        225     988      88      61      93

  Fragments arranged by size: 

                 988     225      93      88      61
 
BsmI GAATG_Cn'

Cuts at:      0     126    1455 
   Size:        126    1329
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     203     225     306     315     323     413     898 
   Size:        137      66      22      81       9       8      90     485

Cuts at:    898    1057    1213    1301    1362    1455 
   Size:        159     156      88      61      93

  Fragments arranged by size: 

                 485     159     156     137      93      90      88      81
                  66      61      22       9       8
 
BspHI T'CATG_A

Cuts at:      0     860    1455 
   Size:        860     595
 
BsrBI CCG'CTC

Cuts at:      0    1317    1455 
   Size:       1317     138
 
BsrFI r'CCGG_y

Cuts at:      0      95     947    1455 
   Size:         95     852     508

  Fragments arranged by size: 

                 852     508      95
 
BssSI C'ACGA_G

Cuts at:      0     224     694    1455 
   Size:        224     470     761

  Fragments arranged by size: 

                 761     470     224
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     852    1455 
   Size:        852     603
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     652     708    1035    1425    1455 
   Size:        652      56     327     390      30

  Fragments arranged by size: 

                 652     390     327      56      30
 
BstYI r'GATC_y

Cuts at:      0     761    1104    1455 
   Size:        761     343     351

  Fragments arranged by size: 

                 761     351     343
 
Bsu36I CC'TnA_GG

Cuts at:      0     163    1455 
   Size:        163    1292
 
BtgI C'CryG_G

Cuts at:      0       5     315     440     651     972    1455 
   Size:          5     310     125     211     321     483

  Fragments arranged by size: 

                 483     321     310     211     125       5
 
BtsI GCAGTG_nn'

Cuts at:      0      35     225     984    1111    1287    1455 
   Size:         35     190     759     127     176     168

  Fragments arranged by size: 

                 759     190     176     168     127      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     852    1455 
   Size:        852     603
 
EaeI y'GGCC_r

Cuts at:      0       2     443     945    1004    1322    1455 
   Size:          2     441     502      59     318     133

  Fragments arranged by size: 

                 502     441     318     133      59       2
 
EarI CTCTTCn'nnn_

Cuts at:      0    1180    1455 
   Size:       1180     275
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      82    1243    1455 
   Size:         82    1161     212

  Fragments arranged by size: 

                1161     212      82
 
EcoICRI GAG'CTC

Cuts at:      0    1211    1455 
   Size:       1211     244
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     882    1204    1392    1455 
   Size:        882     322     188      63

  Fragments arranged by size: 

                 882     322     188      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0     200     303     311     392    1172    1262    1455 
   Size:        200     103       8      81     780      90     193

  Fragments arranged by size: 

                 780     200     193     103      90      81       8
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1209    1455 
   Size:       1209     246
 
FspI TGC'GCA

Cuts at:      0     724    1225    1455 
   Size:        724     501     230

  Fragments arranged by size: 

                 724     501     230
 
FspAI rTGC'GCAy

Cuts at:      0     724    1455 
   Size:        724     731
 
HaeII r_GCGC'y

Cuts at:      0     148     195     782    1455 
   Size:        148      47     587     673

  Fragments arranged by size: 

                 673     587     148      47
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1200    1232    1455 
   Size:       1200      32     223

  Fragments arranged by size: 

                1200     223      32
 
HincII GTy'rAC

Cuts at:      0     813    1338    1455 
   Size:        813     525     117

  Fragments arranged by size: 

                 813     525     117
 
HindIII A'AGCT_T

Cuts at:      0    1191    1455 
   Size:       1191     264
 
MluI A'CGCG_T

Cuts at:      0      47    1455 
   Size:         47    1408
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     686    1455 
   Size:        686     769
 
MscI TGG'CCA

Cuts at:      0    1006    1455 
   Size:       1006     449
 
MslI CAynn'nnrTG

Cuts at:      0     512     650     694     829    1294    1455 
   Size:        512     138      44     135     465     161

  Fragments arranged by size: 

                 512     465     161     138     135      44
 
MspA1I CmG'CkG

Cuts at:      0       7     108     180     354     603    1182    1421    1455 
   Size:          7     101      72     174     249     579     239      34

  Fragments arranged by size: 

                 579     249     239     174     101      72      34       7
            
 
NaeI GCC'GGC

Cuts at:      0      97     949    1455 
   Size:         97     852     506

  Fragments arranged by size: 

                 852     506      97
 
NcoI C'CATG_G

Cuts at:      0     440    1455 
   Size:        440    1015
 
NgoMIV G'CCGG_C

Cuts at:      0      95     947    1455 
   Size:         95     852     508

  Fragments arranged by size: 

                 852     508      95
 
NspI r_CATG'y

Cuts at:      0     489     859    1293    1455 
   Size:        489     370     434     162

  Fragments arranged by size: 

                 489     434     370     162
 
PasI CC'CwG_GG

Cuts at:      0     306    1455 
   Size:        306    1149
 
PciI A'CATG_T

Cuts at:      0    1289    1455 
   Size:       1289     166
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     510    1455 
   Size:        510     945
 
PmlI CAC'GTG

Cuts at:      0     510    1455 
   Size:        510     945
 
PpuMI rG'GwC_Cy

Cuts at:      0    1172    1455 
   Size:       1172     283
 
PspOMI G'GGCC_C

Cuts at:      0     199     302     311    1455 
   Size:        199     103       9    1144

  Fragments arranged by size: 

                1144     199     103       9
 
PstI C_TGCA'G

Cuts at:      0     359    1181    1349    1455 
   Size:        359     822     168     106

  Fragments arranged by size: 

                 822     359     168     106
 
PvuII CAG'CTG

Cuts at:      0     108     180     354     603    1182    1455 
   Size:        108      72     174     249     579     273

  Fragments arranged by size: 

                 579     273     249     174     108      72
 
SacI G_AGCT'C

Cuts at:      0    1213    1455 
   Size:       1213     242
 
SacII CC_GC'GG

Cuts at:      0       8    1455 
   Size:          8    1447
 
SanDI GG'GwC_CC

Cuts at:      0    1172    1455 
   Size:       1172     283
 
SfcI C'TryA_G

Cuts at:      0     231     355    1149    1177    1345    1455 
   Size:        231     124     794      28     168     110

  Fragments arranged by size: 

                 794     231     168     124     110      28
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     310    1455 
   Size:        310    1145
 
SgrAI Cr'CCGG_yG

Cuts at:      0      95    1455 
   Size:         95    1360
 
SmaI CCC'GGG

Cuts at:      0    1102    1455 
   Size:       1102     353
 
StyI C'CwwG_G

Cuts at:      0     125     440     526    1325    1455 
   Size:        125     315      86     799     130

  Fragments arranged by size: 

                 799     315     130     125      86
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     566     633    1455 
   Size:        566      67     822

  Fragments arranged by size: 

                 822     566      67
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1035    1455 
   Size:       1035     420
 
XmaI C'CCGG_G

Cuts at:      0    1100    1455 
   Size:       1100     355
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI
     ApaI    ApaLI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
     BglI Bme1580I    BmgBI     BplI     BpmI    BsaAI    BsaHI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrBI    BsrFI    BssSI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI
     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI
     SfcI     SfiI    SgrAI     SmaI     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I    AflII     AgeI     AhdI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI    BciVI
     BclI    BfrBI    BglII     BlpI     BmrI     BmtI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI    BsmBI
    BspEI    BspMI    BsrDI    BsrGI   BssHII    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI    EcoNI    EcoRI
    EcoRV     FseI     HpaI     KasI     KpnI     MfeI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PfoI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI     SapI
     SbfI     ScaI    SexAI     SfoI     SgfI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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