Restriction map for pF1KB3738
 (Linear) (Six-base) MAPSORT of: KIBB3738.seq  Check: 724  from: 1  to: 1071



KIBB3738.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AflIII A'CryG_T

Cuts at:      0     905    1071 
   Size:        905     166
 
AhdI GACnn_n'nnGTC

Cuts at:      0     523    1071 
   Size:        523     548
 
ApaLI G'TGCA_C

Cuts at:      0      40     747    1071 
   Size:         40     707     324

  Fragments arranged by size: 

                 707     324      40
 
ApoI r'AATT_y

Cuts at:      0     174     310    1071 
   Size:        174     136     761

  Fragments arranged by size: 

                 761     174     136
 
AvaI C'yCGr_G

Cuts at:      0     296    1071 
   Size:        296     775
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     827     860    1071 
   Size:        827      33     211

  Fragments arranged by size: 

                 827     211      33
 
BamHI G'GATC_C

Cuts at:      0     482    1071 
   Size:        482     589
 
BanI G'GyrC_C

Cuts at:      0     454    1071 
   Size:        454     617
 
BanII G_rGCy'C

Cuts at:      0     685     829    1071 
   Size:        685     144     242

  Fragments arranged by size: 

                 685     242     144
 
BbeI G_GCGC'C

Cuts at:      0     458    1071 
   Size:        458     613
 
BbvCI CC'TCA_GC

Cuts at:      0     109    1071 
   Size:        109     962
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     271     305     909     943    1071 
   Size:        271      34     604      34     128

  Fragments arranged by size: 

                 604     271     128      34      34
 
BfrBI ATG'CAT

Cuts at:      0     117    1071 
   Size:        117     954
 
BglII A'GATC_T

Cuts at:      0     668    1071 
   Size:        668     403
 
Bme1580I G_kGCm'C

Cuts at:      0      44     751    1071 
   Size:         44     707     320

  Fragments arranged by size: 

                 707     320      44
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     162    1049    1071 
   Size:        162     887      22

  Fragments arranged by size: 

                 887     162      22
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     649    1003    1015    1071 
   Size:         85     564     354      12      56

  Fragments arranged by size: 

                 564     354      85      56      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     109    1071 
   Size:        109     962
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578    1027    1071 
   Size:        578     449      44

  Fragments arranged by size: 

                 578     449      44
 
BsaAI yAC'GTr

Cuts at:      0     908    1071 
   Size:        908     163
 
BsaHI Gr'CG_yC

Cuts at:      0     455    1071 
   Size:        455     616
 
BseYI C'CCAG_C

Cuts at:      0     201     541     717     822    1071 
   Size:        201     340     176     105     249

  Fragments arranged by size: 

                 340     249     201     176     105
 
BsiHKAI G_wGCw'C

Cuts at:      0      44     294     751    1071 
   Size:         44     250     457     320

  Fragments arranged by size: 

                 457     320     250      44
 
BsmI GAATG_Cn'

Cuts at:      0     117    1071 
   Size:        117     954
 
Bsp1286I G_dGCh'C

Cuts at:      0      44     294     685     751     829    1071 
   Size:         44     250     391      66      78     242

  Fragments arranged by size: 

                 391     250     242      78      66      44
 
BsrDI GCAATG_nn'

Cuts at:      0     587    1071 
   Size:        587     484
 
BsrFI r'CCGG_y

Cuts at:      0     389    1071 
   Size:        389     682
 
BssSI C'ACGA_G

Cuts at:      0     568     808    1071 
   Size:        568     240     263

  Fragments arranged by size: 

                 568     263     240
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     201     582    1071 
   Size:        201     381     489

  Fragments arranged by size: 

                 489     381     201
 
BstYI r'GATC_y

Cuts at:      0     482     668    1071 
   Size:        482     186     403

  Fragments arranged by size: 

                 482     403     186
 
Bsu36I CC'TnA_GG

Cuts at:      0     686    1071 
   Size:        686     385
 
BtgI C'CryG_G

Cuts at:      0     194    1071 
   Size:        194     877
 
DraI TTT'AAA

Cuts at:      0    1014    1071 
   Size:       1014      57
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     612    1071 
   Size:        612     459
 
EaeI y'GGCC_r

Cuts at:      0      11     572     910    1071 
   Size:         11     561     338     161

  Fragments arranged by size: 

                 561     338     161      11
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     518    1071 
   Size:        518     553
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     649    1003    1015    1071 
   Size:         85     564     354      12      56

  Fragments arranged by size: 

                 564     354      85      56      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      86    1071 
   Size:         86     985
 
EcoO109I rG'GnC_Cy

Cuts at:      0     525     803    1071 
   Size:        525     278     268

  Fragments arranged by size: 

                 525     278     268
 
EcoRI G'AATT_C

Cuts at:      0     310    1071 
   Size:        310     761
 
EcoRV GAT'ATC

Cuts at:      0     879    1071 
   Size:        879     192
 
HaeII r_GCGC'y

Cuts at:      0     458    1071 
   Size:        458     613
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     509     541    1071 
   Size:        509      32     530

  Fragments arranged by size: 

                 530     509      32
 
HindIII A'AGCT_T

Cuts at:      0     212     424    1071 
   Size:        212     212     647

  Fragments arranged by size: 

                 647     212     212
 
KasI G'GCGC_C

Cuts at:      0     454    1071 
   Size:        454     617
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     105    1071 
   Size:        105     966
 
MscI TGG'CCA

Cuts at:      0     574     912    1071 
   Size:        574     338     159

  Fragments arranged by size: 

                 574     338     159
 
MspA1I CmG'CkG

Cuts at:      0     717    1071 
   Size:        717     354
 
NarI GG'CG_CC

Cuts at:      0     455    1071 
   Size:        455     616
 
NcoI C'CATG_G

Cuts at:      0     194    1071 
   Size:        194     877
 
NsiI A_TGCA'T

Cuts at:      0     119    1071 
   Size:        119     952
 
NspI r_CATG'y

Cuts at:      0     859    1071 
   Size:        859     212
 
PasI CC'CwG_GG

Cuts at:      0     513    1071 
   Size:        513     558
 
PfoI T'CCnGG_A

Cuts at:      0     866    1071 
   Size:        866     205
 
PmlI CAC'GTG

Cuts at:      0     908    1071 
   Size:        908     163
 
PpuMI rG'GwC_Cy

Cuts at:      0     525     803    1071 
   Size:        525     278     268

  Fragments arranged by size: 

                 525     278     268
 
PstI C_TGCA'G

Cuts at:      0     496    1071 
   Size:        496     575
 
PvuII CAG'CTG

Cuts at:      0     717    1071 
   Size:        717     354
 
RsrII CG'GwC_CG

Cuts at:      0      46    1071 
   Size:         46    1025
 
SanDI GG'GwC_CC

Cuts at:      0     803    1071 
   Size:        803     268
 
SbfI CC_TGCA'GG

Cuts at:      0     496    1071 
   Size:        496     575
 
SexAI A'CCwGG_T

Cuts at:      0     261    1071 
   Size:        261     810
 
SfcI C'TryA_G

Cuts at:      0     492    1071 
   Size:        492     579
 
SfoI GGC'GCC

Cuts at:      0     456    1071 
   Size:        456     615
 
SmlI C'TyrA_G

Cuts at:      0     593    1042    1071 
   Size:        593     449      29

  Fragments arranged by size: 

                 593     449      29
 
SphI G_CATG'C

Cuts at:      0     859    1071 
   Size:        859     212
 
StuI AGG'CCT

Cuts at:      0     763    1000    1071 
   Size:        763     237      71

  Fragments arranged by size: 

                 763     237      71
 
StyI C'CwwG_G

Cuts at:      0     194     638     674    1071 
   Size:        194     444      36     397

  Fragments arranged by size: 

                 444     397     194      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     982    1071 
   Size:        982      89
 
Tth111I GACn'n_nGTC

Cuts at:      0     436    1071 
   Size:        436     635
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     870    1071 
   Size:        870     201
 

 Enzymes that do cut:

   AflIII     AhdI    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BfrBI    BglII Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BsrDI    BsrFI    BssSI    BstXI    BstYI   Bsu36I     BtgI
     DraI     DrdI     EaeI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV    HaeII    Hin4I  HindIII     KasI     MmeI     MscI   MspA1I
     NarI     NcoI     NsiI     NspI     PasI     PfoI     PmlI    PpuMI
     PstI    PvuII    RsrII    SanDI     SbfI    SexAI     SfcI     SfoI
     SmlI     SphI     StuI     StyI    TaqII  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI    AvrII     BbsI    BciVI     BclI     BglI     BlpI    BmgBI
     BmtI     BplI     BsaI    BsaBI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI
    CspCI   DraIII     EagI     EarI  EcoICRI     FalI     FseI     FspI
    FspAI   HincII     HpaI     KpnI     MfeI     MluI     MslI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
     SacI    SacII     SalI     SapI     ScaI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     TatI     TstI
     XbaI     XhoI     XmaI     XmnI     ZraI
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