Restriction map for
pF1KB3737
(Circular) (Six-base) MAPSORT of: pF1KB3737.seq Check: 4778 from: 1 to: 4364
pF1KB3737 4364 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 263 873 1330 263
Size: 610 457 3297
Fragments arranged by size:
3297 610 457
AccI GT'mk_AC
Cuts at: 1402 1402
Size: 4364
Acc65I G'GTAC_C
Cuts at: 1380 1380
Size: 4364
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1269 2189 2621 3368 3636 1269
Size: 920 432 747 268 1997
Fragments arranged by size:
1997 920 747 432 268
AflIII A'CryG_T
Cuts at: 1716 2820 3797 1716
Size: 1104 977 2283
Fragments arranged by size:
2283 1104 977
AgeI A'CCGG_T
Cuts at: 2752 2752
Size: 4364
AlwNI CAG_nnn'CTG
Cuts at: 1669 3236 1669
Size: 1567 2797
ApaI G_GGCC'C
Cuts at: 127 1138 127
Size: 1011 3353
ApaLI G'TGCA_C
Cuts at: 250 641 3134 250
Size: 391 2493 1480
Fragments arranged by size:
2493 1480 391
ApoI r'AATT_y
Cuts at: 1368 3717 3973 1368
Size: 2349 256 1759
Fragments arranged by size:
2349 1759 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4364
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 1384 1384
Size: 4364
BamHI G'GATC_C
Cuts at: 1389 1389
Size: 4364
BanI G'GyrC_C
Cuts at: 929 1187 1380 1794 2042 2077 929
Size: 258 193 414 248 35 3216
Fragments arranged by size:
3216 414 258 248 193 35
BanII G_rGCy'C
Cuts at: 127 1138 1334 1378 2408 127
Size: 1011 196 44 1030 2083
Fragments arranged by size:
2083 1030 1011 196 44
BbeI G_GCGC'C
Cuts at: 1798 2046 1798
Size: 248 4116
BbvCI CC'TCA_GC
Cuts at: 222 222
Size: 4364
BciVI GTATCCnnnnn_n'
Cuts at: 648 2262 3023 4364 648
Size: 1614 761 1341 648
Fragments arranged by size:
1614 1341 761 648
BclI T'GATC_A
Cuts at: 1145 1884 1145
Size: 739 3625
BglI GCCn_nnn'nGGC
Cuts at: 556 556
Size: 4364
BglII A'GATC_T
Cuts at: 979 1879 3916 979
Size: 900 2037 1427
Fragments arranged by size:
2037 1427 900
BlpI GC'TnA_GC
Cuts at: 1476 1476
Size: 4364
Bme1580I G_kGCm'C
Cuts at: 127 254 373 567 645 828 932 1138 1212
Size: 127 119 194 78 183 104 206 74
Cuts at: 1212 1989 2082 3138 127
Size: 777 93 1056 1353
Fragments arranged by size:
1353 1056 777 206 194 183 127 119
104 93 78 74
BmrI ACTGGGnnnn_n'
Cuts at: 130 531 1311 1576 1657 1992 4136 130
Size: 401 780 265 81 335 2144 358
Fragments arranged by size:
2144 780 401 358 335 265 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 4364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 919 1065 804
Size: 115 146 4103
Fragments arranged by size:
4103 146 115
Bpu10I CC'TnA_GC
Cuts at: 222 1072 222
Size: 850 3514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 2419 2911 3209 1540
Size: 879 492 298 2695
Fragments arranged by size:
2695 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 450 4068 450
Size: 3618 746
BsaAI yAC'GTr
Cuts at: 1717 1717
Size: 4364
BsaBI GATnn'nnATC
Cuts at: 6 1878 3915 6
Size: 1872 2037 455
Fragments arranged by size:
2037 1872 455
BsaHI Gr'CG_yC
Cuts at: 1795 2043 2745 4241 1795
Size: 248 702 1496 1918
Fragments arranged by size:
1918 1496 702 248
BsaWI w'CCGG_w
Cuts at: 974 1553 2074 2752 3026 3173 3584 974
Size: 579 521 678 274 147 411 1754
Fragments arranged by size:
1754 678 579 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 484 514 484
Size: 30 4334
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 916 1105 1108 508
Size: 408 189 3 3764
Fragments arranged by size:
3764 408 189 3
BseYI C'CCAG_C
Cuts at: 126 138 474 514 714 835 1307 1789 2547
Size: 12 336 40 200 121 472 482 758
Cuts at: 2547 3124 3620 126
Size: 577 496 870
Fragments arranged by size:
870 758 577 496 482 472 336 200
121 40 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1052 1033
Size: 19 4345
BsiEI CG_ry'CG
Cuts at: 104 762 1906 1952 3160 104
Size: 658 1144 46 1208 1308
Fragments arranged by size:
1308 1208 1144 658 46
BsiHKAI G_wGCw'C
Cuts at: 254 645 1061 1378 2156 2346 3138 254
Size: 391 416 317 778 190 792 1480
Fragments arranged by size:
1480 792 778 416 391 317 190
Bsp1286I G_dGCh'C
Cuts at: 127 254 373 567 645 828 932 1061 1138
Size: 127 119 194 78 183 104 129 77
Cuts at: 1138 1212 1334 1378 1989 2082 2156 2346 2408
Size: 74 122 44 611 93 74 190 62
Cuts at: 2408 3138 127
Size: 730 1353
Fragments arranged by size:
1353 730 611 194 190 183 129 127
122 119 104 93 78 77 74 74
62 44
BspEI T'CCGG_A
Cuts at: 3584 3584
Size: 4364
BspHI T'CATG_A
Cuts at: 3540 3540
Size: 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 263 561 873 1330 1414 1628 1930 2311 263
Size: 298 312 457 84 214 302 381 2316
Fragments arranged by size:
2316 457 381 312 302 298 214 84
BsrBI CCG'CTC
Cuts at: 50 2656 2710 4194 4359 50
Size: 2606 54 1484 165 55
Fragments arranged by size:
2606 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 732 2276 732
Size: 1544 2820
BsrFI r'CCGG_y
Cuts at: 757 1048 2362 2543 2752 757
Size: 291 1314 181 209 2369
Fragments arranged by size:
2369 1314 291 209 181
BssSI C'ACGA_G
Cuts at: 2635 2993 2635
Size: 358 4006
BstAPI GCAn_nnn'nTGC
Cuts at: 430 1741 430
Size: 1311 3053
BstBI TT'CG_AA
Cuts at: 2725 2725
Size: 4364
BstXI CCAn_nnnn'nTGG
Cuts at: 120 120
Size: 4364
BstYI r'GATC_y
Cuts at: 1 440 979 1218 1389 1856 1879 2214 2460
Size: 439 539 239 171 467 23 335 246
Cuts at: 2460 3472 3558 3570 3916 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 539 467 449 439 346 335 246
239 171 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 593 593
Size: 4364
BtgI C'CryG_G
Cuts at: 288 687 1191 1197 288
Size: 399 504 6 3455
Fragments arranged by size:
3455 504 399 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 877 1180 2314 2495 2658 877
Size: 303 1134 181 163 2583
Fragments arranged by size:
2583 1134 303 181 163
BtsI GCAGTG_nn'
Cuts at: 485 1607 485
Size: 1122 3242
DraI TTT'AAA
Cuts at: 1363 1363
Size: 4364
DraIII CAC_nnn'GTG
Cuts at: 232 232
Size: 4364
DrdI GACnn_nn'nnGTC
Cuts at: 2070 2928 2070
Size: 858 3506
EaeI y'GGCC_r
Cuts at: 165 291 759 1194 1252 1949 2123 2514 2541
Size: 126 468 435 58 697 174 391 27
Cuts at: 2541 3886 165
Size: 1345 643
Fragments arranged by size:
1345 697 643 468 435 391 174 126
58 27
EagI C'GGCC_G
Cuts at: 759 1949 759
Size: 1190 3174
EarI CTCTTCn'nnn_
Cuts at: 835 2387 2597 835
Size: 1552 210 2602
Fragments arranged by size:
2602 1552 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 952 2572 2882 3028 4174 952
Size: 1620 310 146 1146 1142
Fragments arranged by size:
1620 1146 1142 310 146
EcoICRI GAG'CTC
Cuts at: 1376 1376
Size: 4364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 919 1065 1269 2189 2621 3368 3636 804
Size: 115 146 204 920 432 747 268 1532
Fragments arranged by size:
1532 920 747 432 268 204 146 115
EcoNI CCTnn'n_nnAGG
Cuts at: 178 901 997 178
Size: 723 96 3545
Fragments arranged by size:
3545 723 96
EcoO109I rG'GnC_Cy
Cuts at: 183 1068 1135 1503 183
Size: 885 67 368 3044
Fragments arranged by size:
3044 885 368 67
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4364
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4364
HaeII r_GCGC'y
Cuts at: 1206 1798 2046 3068 4047 1206
Size: 592 248 1022 979 1523
Fragments arranged by size:
1523 1022 979 592 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1264 1296 2206 2236 2238 2268 1264
Size: 32 910 30 2 30 3360
Fragments arranged by size:
3360 910 32 30 30 2
HincII GTy'rAC
Cuts at: 1403 1403
Size: 4364
KasI G'GCGC_C
Cuts at: 1794 2042 1794
Size: 248 4116
KpnI G_GTAC'C
Cuts at: 1384 1384
Size: 4364
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 773 912 3035 3219 773
Size: 139 2123 184 1918
Fragments arranged by size:
2123 1918 184 139
MscI TGG'CCA
Cuts at: 167 293 1196 1254 2125 167
Size: 126 903 58 871 2406
Fragments arranged by size:
2406 903 871 126 58
MslI CAynn'nnrTG
Cuts at: 1024 1109 2353 2480 1024
Size: 85 1244 127 2908
Fragments arranged by size:
2908 1244 127 85
MspA1I CmG'CkG
Cuts at: 764 1185 1289 1475 1789 2149 3162 3407 764
Size: 421 104 186 314 360 1013 245 1721
Fragments arranged by size:
1721 1013 421 360 314 245 186 104
NaeI GCC'GGC
Cuts at: 1050 2545 1050
Size: 1495 2869
NarI GG'CG_CC
Cuts at: 1795 2043 1795
Size: 248 4116
NcoI C'CATG_G
Cuts at: 687 1191 687
Size: 504 3860
NgoMIV G'CCGG_C
Cuts at: 1048 2543 1048
Size: 1495 2869
NspI r_CATG'y
Cuts at: 1417 1917 2824 1417
Size: 500 907 2957
Fragments arranged by size:
2957 907 500
PasI CC'CwG_GG
Cuts at: 365 365
Size: 4364
PciI A'CATG_T
Cuts at: 2820 2820
Size: 4364
PflMI CCAn_nnn'nTGG
Cuts at: 721 721
Size: 4364
PfoI T'CCnGG_A
Cuts at: 1221 1221
Size: 4364
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 4332
PpuMI rG'GwC_Cy
Cuts at: 183 1068 183
Size: 885 3479
PspOMI G'GGCC_C
Cuts at: 123 1134 123
Size: 1011 3353
PstI C_TGCA'G
Cuts at: 1177 1411 2096 1177
Size: 234 685 3445
Fragments arranged by size:
3445 685 234
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 1289 1789 2149 1289
Size: 500 360 3504
Fragments arranged by size:
3504 500 360
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 1378 1378
Size: 4364
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4364
SapI GCTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4154
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4364
ScaI AGT'ACT
Cuts at: 3881 3881
Size: 4364
SexAI A'CCwGG_T
Cuts at: 1118 1118
Size: 4364
SfcI C'TryA_G
Cuts at: 33 1173 1407 2092 3085 3276 33
Size: 1140 234 685 993 191 1121
Fragments arranged by size:
1140 1121 993 685 234 191
SfoI GGC'GCC
Cuts at: 1796 2044 1796
Size: 248 4116
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SmaI CCC'GGG
Cuts at: 1386 1386
Size: 4364
SmlI C'TyrA_G
Cuts at: 1519 2434 2926 3188 1519
Size: 915 492 262 2695
Fragments arranged by size:
2695 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1417 1917 1417
Size: 500 3864
StuI AGG'CCT
Cuts at: 613 880 907 1338 613
Size: 267 27 431 3639
Fragments arranged by size:
3639 431 267 27
StyI C'CwwG_G
Cuts at: 687 1130 1191 1266 1498 1852 687
Size: 443 61 75 232 354 3199
Fragments arranged by size:
3199 443 354 232 75 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 867 1947 2080 2539 2748 867
Size: 1080 133 459 209 2483
Fragments arranged by size:
2483 1080 459 209 133
TatI w'GTAC_w
Cuts at: 3879 3879
Size: 4364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 484 516 3881 3913 484
Size: 32 3365 32 935
Fragments arranged by size:
3365 935 32 32
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4364
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1164 1189 1859 1164
Size: 25 670 3669
Fragments arranged by size:
3669 670 25
XmaI C'CCGG_G
Cuts at: 1384 1384
Size: 4364
XmnI GAAnn'nnTTC
Cuts at: 1238 1282 1238
Size: 44 4320
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI