Restriction map for pF1KB3659
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        Alw44I      
AlwNI       AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspHI       AviII       AvrII       BalI        BbeI        
BbrPI       BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BssHII      Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FspI        HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tru9I       Tsp509I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII         330  CGCG                 CG^CG
AcyI          490  GACGCC               G[GA]^CG[CT]C
AhdI          210  GACTGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         210  GACTGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          298  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          448  CTCGGG               C^[CT]CG[GA]G
BamHI         850  GGATCC               G^GATCC
BanI          187  GGCACC               G^G[CT][GA]CC
BbsI          723  GAAGAC               GAAGAC(2/6)
BbvCI         583  GCTGAGG              CCTCAGC(-5/-2)
BcgI          440  GCATACACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         723  GAAGAC               GAAGAC(2/6)
BsaHI         490  GACGCC               G[GA]^CG[CT]C
BsmAI         664  GTCTC                GTCTC(1/5)
BsmI          564  GAATGC               GAATGC(1/-1)
BsoBI         448  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      314  TGTACA               T^GTACA
BspHI         557  TCATGA               T^CATGA
BsrGI         314  TGTACA               T^GTACA
BssSI         163  CTCGTG               CACGAG(-5/-1)
BstUI         330  CGCG                 CG^CG
Eam1105I      210  GACTGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI          479  GCGGG                CCCGC(4/6)
FseI          246  GGCCGGCC             GGCCGG^CC
HaeII         257  AGCGCC               [GA]GCGC^[CT]
HgaI          490  GACGC                GACGC(5/10)
Hin1I         490  GACGCC               G[GA]^CG[CT]C
HincII        574  GTCAAC               GT[CT]^[GA]AC
HindII        574  GTCAAC               GT[CT]^[GA]AC
MboII         723  GAAGA                GAAGA(8/7)
MvnI          330  CGCG                 CG^CG
PflFI         298  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         216  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         886  CAGCTG               CAG^CTG
RcaI          557  TCATGA               T^CATGA
ScaI          755  AGTACT               AGT^ACT
SfaNI         284  GCATC                GCATC(5/9)
SmlI          607  CTCAAG               C^T[CT][GA]AG
SspBI         314  TGTACA               T^GTACA
TaqI          770  TCGA                 T^CGA
TfiI          925  GAATC                G^A[AT]TC
Tth111I       298  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       770  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

ApaI          801  GGGCCC               GGGCC^C
              896  GGGCCC              
BanII         801  GGGCCC               G[GA]GC[CT]^C
              896  GGGCCC              
BpmI          339  CTCCAG               CTGGAG(16/14)
              746  CTCCAG              
BsgI          369  CTGCAC               GTGCAG(16/14)
              166  GTGCAG              
BsmFI         481  GGGAC                GGGAC(10/14)
              218  GTCCC               
BspMI         632  ACCTGC               ACCTGC(4/8)
              309  GCAGGT              
BsrI           99  ACTGG                ACTGG(1/-1)
              753  CCAGT               
BstEII        390  GGTGACC              G^GT[ACGT]ACC
              591  GGTGACC             
BstPI         390  GGTGACC              G^GT[ACGT]ACC
              591  GGTGACC             
BstXI         407  CCACAGATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              692  CCACAGTGGTGG        
BstYI         125  AGATCC               [GA]^GATC[CT]
              850  GGATCC              
CfoI          258  GCGC                 GCG^C
              329  GCGC                
DdeI          212  CTGAG                C^T[ACGT]AG
              584  CTGAG               
DpnII         126  GATC                 ^GATC
              851  GATC                
EaeI          245  TGGCCG               [CT]^GGCC[GA]
              362  TGGCCG              
EcoO65I       390  GGTGACC              G^GT[ACGT]ACC
              591  GGTGACC             
HhaI          258  GCGC                 GCG^C
              329  GCGC                
HinP1I        258  GCGC                 G^CGC
              329  GCGC                
MboI          126  GATC                 ^GATC
              851  GATC                
MflI          125  AGATCC               [GA]^GATC[CT]
              850  GGATCC              
MspA1I        477  CAGCGG               C[AC]G^C[GT]G
              886  CAGCTG              
NdeII         126  GATC                 ^GATC
              851  GATC                
PleI          298  GACTC                GAGTC(4/5)
              521  GAGTC               
Sau3AI        126  GATC                 ^GATC
              851  GATC                
Tsp45I        391  GTGAC                ^GT[GC]AC
              592  GTGAC               
XcmI          191  CCATCCTGACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              374  CCACAGGAGCCATGG     
XhoII         125  AGATCC               [GA]^GATC[CT]
              850  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrFI       BssKI       BstF5I      
BstNI       Cac8I       Cfr10I      Cfr13I      DraII       
DsaI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MnlI        
MslI        MspI        MvaI        MwoI        NaeI        
NciI        NcoI        NgoMIV      NlaIII      NlaIV       
PstI        RsaI        Sau96I      ScrFI       SfcI        
StyI        TseI        TspRI       
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