Restriction map for pF1KB3659
 (Linear) (Six-base) MAPSORT of: KIBB3659.seq  Check: 1107  from: 1  to: 936



KIBB3659.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     300     641     936 
   Size:        300     341     295

  Fragments arranged by size: 

                 341     300     295
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2      31     744     936 
   Size:          2      29     713     192

  Fragments arranged by size: 

                 713     192      29       2
 
AhdI GACnn_n'nnGTC

Cuts at:      0     215     936 
   Size:        215     721
 
AleI CACnn'nnGTG

Cuts at:      0     412     697     936 
   Size:        412     285     239

  Fragments arranged by size: 

                 412     285     239
 
ApaI G_GGCC'C

Cuts at:      0     805     900     936 
   Size:        805      95      36

  Fragments arranged by size: 

                 805      95      36
 
AvaI C'yCGr_G

Cuts at:      0     448     936 
   Size:        448     488
 
BamHI G'GATC_C

Cuts at:      0     850     936 
   Size:        850      86
 
BanI G'GyrC_C

Cuts at:      0     187     936 
   Size:        187     749
 
BanII G_rGCy'C

Cuts at:      0     805     900     936 
   Size:        805      95      36

  Fragments arranged by size: 

                 805      95      36
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     730     936 
   Size:        730     206
 
BbvCI CC'TCA_GC

Cuts at:      0     584     936 
   Size:        584     352
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     429     463     936 
   Size:        429      34     473

  Fragments arranged by size: 

                 473     429      34
 
Bme1580I G_kGCm'C

Cuts at:      0     190     402     805     900     936 
   Size:        190     212     403      95      36

  Fragments arranged by size: 

                 403     212     190      95      36
 
BmgBI CAC'GTC

Cuts at:      0      24     717     936 
   Size:         24     693     219

  Fragments arranged by size: 

                 693     219      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     324     731     936 
   Size:        324     407     205

  Fragments arranged by size: 

                 407     324     205
 
Bpu10I CC'TnA_GC

Cuts at:      0     584     936 
   Size:        584     352
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     592     936 
   Size:        592     344
 
BsaHI Gr'CG_yC

Cuts at:      0     491     936 
   Size:        491     445
 
BseYI C'CCAG_C

Cuts at:      0     183     401     936 
   Size:        183     218     535

  Fragments arranged by size: 

                 535     218     183
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     354     936 
   Size:        187     167     582

  Fragments arranged by size: 

                 582     187     167
 
BsmI GAATG_Cn'

Cuts at:      0     570     936 
   Size:        570     366
 
Bsp1286I G_dGCh'C

Cuts at:      0     190     402     805     900     936 
   Size:        190     212     403      95      36

  Fragments arranged by size: 

                 403     212     190      95      36
 
BspHI T'CATG_A

Cuts at:      0     557     936 
   Size:        557     379
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     300     641     936 
   Size:        300     341     295

  Fragments arranged by size: 

                 341     300     295
 
BsrFI r'CCGG_y

Cuts at:      0     247     364     679     936 
   Size:        247     117     315     257

  Fragments arranged by size: 

                 315     257     247     117
 
BsrGI T'GTAC_A

Cuts at:      0     314     936 
   Size:        314     622
 
BssSI C'ACGA_G

Cuts at:      0     163     936 
   Size:        163     773
 
BstEII G'GTnAC_C

Cuts at:      0     390     591     936 
   Size:        390     201     345

  Fragments arranged by size: 

                 390     345     201
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     414     699     936 
   Size:        414     285     237

  Fragments arranged by size: 

                 414     285     237
 
BstYI r'GATC_y

Cuts at:      0     125     850     936 
   Size:        125     725      86

  Fragments arranged by size: 

                 725     125      86
 
BtgI C'CryG_G

Cuts at:      0      35     174     200     383     936 
   Size:         35     139      26     183     553

  Fragments arranged by size: 

                 553     183     139      35      26
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     489     936 
   Size:        489     447
 
EaeI y'GGCC_r

Cuts at:      0     245     362     936 
   Size:        245     117     574

  Fragments arranged by size: 

                 574     245     117
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     106     936 
   Size:        106     830
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2      31     324     731     744     936 
   Size:          2      29     293     407      13     192

  Fragments arranged by size: 

                 407     293     192      29      13       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     217     528     801     802     897     936 
   Size:        217     311     273       1      95      39

  Fragments arranged by size: 

                 311     273     217      95      39       1
 
FseI GG_CCGG'CC

Cuts at:      0     251     936 
   Size:        251     685
 
HaeII r_GCGC'y

Cuts at:      0     261     936 
   Size:        261     675
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     201     233     936 
   Size:        201      32     703

  Fragments arranged by size: 

                 703     201      32
 
HincII GTy'rAC

Cuts at:      0     576     936 
   Size:        576     360
 
MslI CAynn'nnrTG

Cuts at:      0      40     388     412     697     936 
   Size:         40     348      24     285     239

  Fragments arranged by size: 

                 348     285     239      40      24
 
MspA1I CmG'CkG

Cuts at:      0     479     888     936 
   Size:        479     409      48

  Fragments arranged by size: 

                 479     409      48
 
NaeI GCC'GGC

Cuts at:      0     249     366     681     936 
   Size:        249     117     315     255

  Fragments arranged by size: 

                 315     255     249     117
 
NcoI C'CATG_G

Cuts at:      0      35     200     383     936 
   Size:         35     165     183     553

  Fragments arranged by size: 

                 553     183     165      35
 
NgoMIV G'CCGG_C

Cuts at:      0     247     364     679     936 
   Size:        247     117     315     257

  Fragments arranged by size: 

                 315     257     247     117
 
PfoI T'CCnGG_A

Cuts at:      0     457     853     936 
   Size:        457     396      83

  Fragments arranged by size: 

                 457     396      83
 
PpuMI rG'GwC_Cy

Cuts at:      0     217     936 
   Size:        217     719
 
PspOMI G'GGCC_C

Cuts at:      0     801     896     936 
   Size:        801      95      40

  Fragments arranged by size: 

                 801      95      40
 
PstI C_TGCA'G

Cuts at:      0     125     226     257     936 
   Size:        125     101      31     679

  Fragments arranged by size: 

                 679     125     101      31
 
PvuII CAG'CTG

Cuts at:      0     888     936 
   Size:        888      48
 
SanDI GG'GwC_CC

Cuts at:      0     217     936 
   Size:        217     719
 
ScaI AGT'ACT

Cuts at:      0     757     936 
   Size:        757     179
 
SfcI C'TryA_G

Cuts at:      0     121     222     253     495     936 
   Size:        121     101      31     242     441

  Fragments arranged by size: 

                 441     242     121     101      31
 
SmlI C'TyrA_G

Cuts at:      0     607     936 
   Size:        607     329
 
StyI C'CwwG_G

Cuts at:      0      35     110     200     383     936 
   Size:         35      75      90     183     553

  Fragments arranged by size: 

                 553     183      90      75      35
 
TatI w'GTAC_w

Cuts at:      0     314     755     936 
   Size:        314     441     181

  Fragments arranged by size: 

                 441     314     181
 
Tth111I GACn'n_nGTC

Cuts at:      0     301     936 
   Size:        301     635
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     198     381     936 
   Size:        198     183     555

  Fragments arranged by size: 

                 555     198     183
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AleI     ApaI     AvaI    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI Bme1580I    BmgBI     BpmI   Bpu10I
    BpuEI    BsaHI    BseYI     BsgI     BsmI Bsp1286I    BspHI    BspMI
    BsrFI    BsrGI    BssSI   BstEII    BstXI    BstYI     BtgI    BtgZI
     EaeI     EciI  Eco57MI EcoO109I     FseI    HaeII    Hin4I   HincII
     MslI   MspA1I     NaeI     NcoI   NgoMIV     PfoI    PpuMI   PspOMI
     PstI    PvuII    SanDI     ScaI     SfcI     SmlI     StyI     TatI
  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrBI
    BsrDI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NarI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI     XbaI     XhoI     XmaI
     XmnI     ZraI
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