Restriction map for pF1KB3655
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       BanI        BbeI        BbrPI       BbvCI       
BclI        BfrI        BglI        BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BspHI       BsrBI       
BsrFI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SwaI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcsI          536  GAATTC               [GA]^AATT[CT]
AflIII        803  ACATGT               A^C[GA][CT]GT
AlwNI         293  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          172  CCTGAGG              CC^T[ACGT]AGG
ApoI          536  GAATTC               [GA]^AATT[CT]
Asp700I      1169  GAACCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1152  GAGCTC               G[AT]GC[AT]^C
AvaI         1229  CCCGAG               C^[CT]CG[GA]G
AvrII        1503  CCTAGG               C^CTAGG
BanII        1152  GAGCTC               G[GA]GC[CT]^C
BbsI         1282  GTCTTC               GAAGAC(2/6)
BcgI          858  GCAGGAAGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         420  GTATCC               GTATCC(6/5)
BcnI         1497  CCGGG                CC^[GC]GG
BglII         161  AGATCT               A^GATCT
BlnI         1503  CCTAGG               C^CTAGG
BlpI          500  GCTCAGC              GC^T[ACGT]AGC
BmyI         1152  GAGCTC               G[AGT]GC[ACT]^C
Bpu1102I      500  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1282  GTCTTC               GAAGAC(2/6)
BsgI         1109  CTGCAC               GTGCAG(16/14)
BsiHKAI      1152  GAGCTC               G[AT]GC[AT]^C
BsmAI         943  GTCTC                GTCTC(1/5)
BsmI          813  GCATTC               GAATGC(1/-1)
BsoBI        1229  CCCGAG               C^[CT]CG[GA]G
Bsp1286I     1152  GAGCTC               G[AGT]GC[ACT]^C
Bsp1407I     1000  TGTACA               T^GTACA
BspLU11I      803  ACATGT               A^CATGT
BsrGI        1000  TGTACA               T^GTACA
BssSI         434  CACGAG               CACGAG(-5/-1)
BstXI         332  CCACCCTTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        172  CCTGAGG              CC^T[ACGT]AGG
CelII         500  GCTCAGC              GC^T[ACGT]AGC
DsaI         1668  CCGTGG               C^C[GA][CT]GG
EarI          589  CTCTTC               CTCTTC(1/4)
Eco81I        172  CCTGAGG              CC^T[ACGT]AGG
EcoRI         536  GAATTC               G^AATTC
Ksp632I       589  CTCTTC               CTCTTC(1/4)
MaeII        1676  ACGT                 A^CGT
MspA1I        355  CAGCGG               C[AC]G^C[GT]G
NciI         1497  CCGGG                CC^[GC]GG
PstI         1142  CTGCAG               CTGCA^G
SacI         1152  GAGCTC               GAGCT^C
SexAI         225  ACCAGGT              A^CC[AT]GGT
SmlI          709  CTCAAG               C^T[CT][GA]AG
SspBI        1000  TGTACA               T^GTACA
SspI          265  AATATT               AAT^ATT
StuI         1644  AGGCCT               AGG^CCT
Tsp509I       537  AATT                 ^AATT
XbaI          832  TCTAGA               T^CTAGA
XcmI          977  CCAACGAGCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1169  GAACCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BalI          329  TGGCCA               TGG^CCA
             1179  TGGCCA              
BsaWI         220  ACCGGA               [AT]^CCGG[AT]
              845  ACCGGA              
BspMI         809  ACCTGC               ACCTGC(4/8)
              984  GCAGGT              
EcoT14I       425  CCAAGG               C^C[AT][AT]GG
             1503  CCTAGG              
MluNI         329  TGGCCA               TGG^CCA
             1179  TGGCCA              
MscI          329  TGGCCA               TGG^CCA
             1179  TGGCCA              
MwoI          348  GCTGCTCCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              351  GCTCCAGCGGC         
StyI          425  CCAAGG               C^C[AT][AT]GG
             1503  CCTAGG              
TaqI          867  TCGA                 T^CGA
             1048  TCGA                
TthHB8I       867  TCGA                 T^CGA
             1048  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BamHI       BbvI        BfaI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmFI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        TfiI        
Tru9I       TseI        Tsp45I      TspRI       XhoII       
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