Restriction map for pF1KB3655
 (Linear) (Six-base) MAPSORT of: KIBB3655.seq  Check: 882  from: 1  to: 1683



KIBB3655.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     975    1683 
   Size:        975     708
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422     977    1683 
   Size:        422     555     706

  Fragments arranged by size: 

                 706     555     422
 
AflIII A'CryG_T

Cuts at:      0     803    1683 
   Size:        803     880
 
AleI CACnn'nnGTG

Cuts at:      0     337    1683 
   Size:        337    1346
 
AlwNI CAG_nnn'CTG

Cuts at:      0     298    1683 
   Size:        298    1385
 
ApoI r'AATT_y

Cuts at:      0     536    1683 
   Size:        536    1147
 
AvaI C'yCGr_G

Cuts at:      0    1229    1683 
   Size:       1229     454
 
AvrII C'CTAG_G

Cuts at:      0    1503    1683 
   Size:       1503     180
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     566     599    1683 
   Size:        566      33    1084

  Fragments arranged by size: 

                1084     566      33
 
BamHI G'GATC_C

Cuts at:      0     255     317    1133    1683 
   Size:        255      62     816     550

  Fragments arranged by size: 

                 816     550     255      62
 
BanII G_rGCy'C

Cuts at:      0    1156    1683 
   Size:       1156     527
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1275    1683 
   Size:       1275     408
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     847     881    1683 
   Size:        847      34     802

  Fragments arranged by size: 

                 847     802      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     431    1683 
   Size:        431    1252
 
BglII A'GATC_T

Cuts at:      0     161    1683 
   Size:        161    1522
 
BlpI GC'TnA_GC

Cuts at:      0     501    1683 
   Size:        501    1182
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     382     395    1606    1683 
   Size:        382      13    1211      77

  Fragments arranged by size: 

                1211     382      77      13
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     180     201    1683 
   Size:        180      21    1482

  Fragments arranged by size: 

                1482     180      21
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     308     337    1683 
   Size:         65     243      29    1346

  Fragments arranged by size: 

                1346     243      65      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     694    1683 
   Size:        694     989
 
BsaWI w'CCGG_w

Cuts at:      0     220     845    1683 
   Size:        220     625     838

  Fragments arranged by size: 

                 838     625     220
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     145     190     193    1683 
   Size:        145      45       3    1490

  Fragments arranged by size: 

                1490     145      45       3
 
BseYI C'CCAG_C

Cuts at:      0       6     691    1086    1683 
   Size:          6     685     395     597

  Fragments arranged by size: 

                 685     597     395       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1094    1683 
   Size:       1094     589
 
BsiHKAI G_wGCw'C

Cuts at:      0    1156    1683 
   Size:       1156     527
 
BsmI GAATG_Cn'

Cuts at:      0     813    1683 
   Size:        813     870
 
Bsp1286I G_dGCh'C

Cuts at:      0    1156    1683 
   Size:       1156     527
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     818     975    1683 
   Size:        818     157     708

  Fragments arranged by size: 

                 818     708     157
 
BsrDI GCAATG_nn'

Cuts at:      0     242     506     566     902    1588    1683 
   Size:        242     264      60     336     686      95

  Fragments arranged by size: 

                 686     336     264     242      95      60
 
BsrGI T'GTAC_A

Cuts at:      0    1000    1683 
   Size:       1000     683
 
BssSI C'ACGA_G

Cuts at:      0     434    1683 
   Size:        434    1249
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     339    1683 
   Size:        339    1344
 
BstYI r'GATC_y

Cuts at:      0     161     255     317    1133    1683 
   Size:        161      94      62     816     550

  Fragments arranged by size: 

                 816     550     161      94      62
 
Bsu36I CC'TnA_GG

Cuts at:      0     173    1683 
   Size:        173    1510
 
BtgI C'CryG_G

Cuts at:      0    1668    1683 
   Size:       1668      15
 
BtsI GCAGTG_nn'

Cuts at:      0     299    1133    1683 
   Size:        299     834     550

  Fragments arranged by size: 

                 834     550     299
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1254    1289    1683 
   Size:       1254      35     394

  Fragments arranged by size: 

                1254     394      35
 
EaeI y'GGCC_r

Cuts at:      0     329    1179    1390    1683 
   Size:        329     850     211     293

  Fragments arranged by size: 

                 850     329     293     211
 
EarI CTCTTCn'nnn_

Cuts at:      0     595    1683 
   Size:        595    1088
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1443    1683 
   Size:       1443     240
 
EcoICRI GAG'CTC

Cuts at:      0    1154    1683 
   Size:       1154     529
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     308     337     422     977    1683 
   Size:         65     243      29      85     555     706

  Fragments arranged by size: 

                 706     555     243      85      65      29
 
EcoO109I rG'GnC_Cy

Cuts at:      0      10     429    1500    1683 
   Size:         10     419    1071     183

  Fragments arranged by size: 

                1071     419     183      10
 
EcoRI G'AATT_C

Cuts at:      0     536    1683 
   Size:        536    1147
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     268     300     773     805    1683 
   Size:        268      32     473      32     878

  Fragments arranged by size: 

                 878     473     268      32      32
 
MscI TGG'CCA

Cuts at:      0     331    1181    1683 
   Size:        331     850     502

  Fragments arranged by size: 

                 850     502     331
 
MslI CAynn'nnrTG

Cuts at:      0     337     649    1471    1683 
   Size:        337     312     822     212

  Fragments arranged by size: 

                 822     337     312     212
 
MspA1I CmG'CkG

Cuts at:      0     357    1683 
   Size:        357    1326
 
NspI r_CATG'y

Cuts at:      0     486     720     807    1513    1605    1683 
   Size:        486     234      87     706      92      78

  Fragments arranged by size: 

                 706     486     234      92      87      78
 
PasI CC'CwG_GG

Cuts at:      0     118    1683 
   Size:        118    1565
 
PciI A'CATG_T

Cuts at:      0     803    1683 
   Size:        803     880
 
PfoI T'CCnGG_A

Cuts at:      0     258    1496    1610    1683 
   Size:        258    1238     114      73

  Fragments arranged by size: 

                1238     258     114      73
 
PpuMI rG'GwC_Cy

Cuts at:      0      10     429    1500    1683 
   Size:         10     419    1071     183

  Fragments arranged by size: 

                1071     419     183      10
 
PstI C_TGCA'G

Cuts at:      0    1146    1683 
   Size:       1146     537
 
SacI G_AGCT'C

Cuts at:      0    1156    1683 
   Size:       1156     527
 
ScaI AGT'ACT

Cuts at:      0     136     184     883    1683 
   Size:        136      48     699     800

  Fragments arranged by size: 

                 800     699     136      48
 
SexAI A'CCwGG_T

Cuts at:      0     225    1683 
   Size:        225    1458
 
SfcI C'TryA_G

Cuts at:      0      90     527    1142    1333    1683 
   Size:         90     437     615     191     350

  Fragments arranged by size: 

                 615     437     350     191      90
 
SmlI C'TyrA_G

Cuts at:      0     709    1683 
   Size:        709     974
 
SspI AAT'ATT

Cuts at:      0     267    1683 
   Size:        267    1416
 
StuI AGG'CCT

Cuts at:      0    1646    1683 
   Size:       1646      37
 
StyI C'CwwG_G

Cuts at:      0     425    1503    1683 
   Size:        425    1078     180

  Fragments arranged by size: 

                1078     425     180
 
TatI w'GTAC_w

Cuts at:      0     134     182     881    1000    1475    1683 
   Size:        134      48     699     119     475     208

  Fragments arranged by size: 

                 699     475     208     134     119      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     425     457    1683 
   Size:        425      32    1226

  Fragments arranged by size: 

                1226     425      32
 
XbaI T'CTAG_A

Cuts at:      0     832    1683 
   Size:        832     851
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     984    1683 
   Size:        984     699
 
XmnI GAAnn'nnTTC

Cuts at:      0    1173    1683 
   Size:       1173     510
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI    AlwNI     ApoI     AvaI    AvrII
     BaeI    BamHI    BanII     BbsI     BcgI    BciVI    BglII     BlpI
     BmrI     BplI     BpmI    BpuEI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrGI    BssSI    BstXI
    BstYI   Bsu36I     BtgI     BtsI    CspCI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI    Hin4I     MscI     MslI   MspA1I
     NspI     PasI     PciI     PfoI    PpuMI     PstI     SacI     ScaI
    SexAI     SfcI     SmlI     SspI     StuI     StyI     TatI     TstI
     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     BanI
     BbeI    BbvCI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmtI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I    BtgZI     ClaI     DraI   DraIII     DrdI     EagI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI    TaqII  Tth111I     XhoI     XmaI
     ZraI
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