Restriction map for pF1KB3488
 (Circular) (Six-base) MAPSORT of: pF1KB3488.seq  Check: 5087  from: 1  to: 4163



pF1KB3488  4163 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:   1201    1201 
   Size:       4163
 
Acc65I G'GTAC_C

Cuts at:   1179    1179 
   Size:       4163
 
AclI AA'CG_TT

Cuts at:    719    4003     719 
   Size:       3284     879
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1147    1988    2420    3167    3435    1147 
   Size:        841     432     747     268    1875

  Fragments arranged by size: 

                1875     841     747     432     268
 
AflIII A'CryG_T

Cuts at:   1515    2619    3596    1515 
   Size:       1104     977    2082

  Fragments arranged by size: 

                2082    1104     977
 
AgeI A'CCGG_T

Cuts at:   2551    2551 
   Size:       4163
 
AleI CACnn'nnGTG

Cuts at:    317     317 
   Size:       4163
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    657     689     657 
   Size:         32    4131
 
AlwNI CAG_nnn'CTG

Cuts at:   1033    1468    3035    1033 
   Size:        435    1567    2161

  Fragments arranged by size: 

                2161    1567     435
 
ApaLI G'TGCA_C

Cuts at:   2933    2933 
   Size:       4163
 
ApoI r'AATT_y

Cuts at:    450    1167    3516    3772     450 
   Size:        717    2349     256     841

  Fragments arranged by size: 

                2349     841     717     256
 
AseI AT'TA_AT

Cuts at:     20     587      20 
   Size:        567    3596
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4163
 
AvaI C'yCGr_G

Cuts at:   1183    1183 
   Size:       4163
 
AvrII C'CTAG_G

Cuts at:    681     681 
   Size:       4163
 
BamHI G'GATC_C

Cuts at:   1188    1188 
   Size:       4163
 
BanI G'GyrC_C

Cuts at:   1179    1593    1841    1876    1179 
   Size:        414     248      35    3466

  Fragments arranged by size: 

                3466     414     248      35
 
BanII G_rGCy'C

Cuts at:   1177    2207    1177 
   Size:       1030    3133
 
BbeI G_GCGC'C

Cuts at:   1597    1845    1597 
   Size:        248    3915
 
BbvCI CC'TCA_GC

Cuts at:   1102    1102 
   Size:       4163
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    325     359     325 
   Size:         34    4129
 
BciVI GTATCCnnnnn_n'

Cuts at:   2061    2822    4163    2061 
   Size:        761    1341    2061

  Fragments arranged by size: 

                2061    1341     761
 
BclI T'GATC_A

Cuts at:    884     899    1683     884 
   Size:         15     784    3364

  Fragments arranged by size: 

                3364     784      15
 
BglII A'GATC_T

Cuts at:   1678    3715    1678 
   Size:       2037    2126
 
BlpI GC'TnA_GC

Cuts at:   1275    1275 
   Size:       4163
 
Bme1580I G_kGCm'C

Cuts at:   1788    1881    2937    1788 
   Size:         93    1056    3014

  Fragments arranged by size: 

                3014    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1375    1456    1791    3935    1375 
   Size:         81     335    2144    1603

  Fragments arranged by size: 

                2144    1603     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2530    2530 
   Size:       4163
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    539    1010    1055     539 
   Size:        471      45    3647

  Fragments arranged by size: 

                3647     471      45
 
Bpu10I CC'TnA_GC

Cuts at:   1102    1102 
   Size:       4163
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1339    2218    2710    3008    1339 
   Size:        879     492     298    2494

  Fragments arranged by size: 

                2494     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3867    3867 
   Size:       4163
 
BsaAI yAC'GTr

Cuts at:   1516    1516 
   Size:       4163
 
BsaBI GATnn'nnATC

Cuts at:      6    1677    3714       6 
   Size:       1671    2037     455

  Fragments arranged by size: 

                2037    1671     455
 
BsaHI Gr'CG_yC

Cuts at:   1594    1842    2544    4040    1594 
   Size:        248     702    1496    1717

  Fragments arranged by size: 

                1717    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1352    1873    2551    2825    2972    3383    1352 
   Size:        521     678     274     147     411    2132

  Fragments arranged by size: 

                2132     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    154     184     902     932     154 
   Size:         30     718      30    3385

  Fragments arranged by size: 

                3385     718      30      30
 
BseYI C'CCAG_C

Cuts at:    245    1012    1588    2346    2923    3419     245 
   Size:        767     576     758     577     496     989

  Fragments arranged by size: 

                 989     767     758     577     576     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    730     730 
   Size:       4163
 
BsiEI CG_ry'CG

Cuts at:    104    1705    1751    2959     104 
   Size:       1601      46    1208    1308

  Fragments arranged by size: 

                1601    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1177    1955    2145    2937    1177 
   Size:        778     190     792    2403

  Fragments arranged by size: 

                2403     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    671     731     671 
   Size:         60    4103
 
Bsp1286I G_dGCh'C

Cuts at:   1177    1788    1881    1955    2145    2207    2937    1177 
   Size:        611      93      74     190      62     730    2403

  Fragments arranged by size: 

                2403     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3383    3383 
   Size:       4163
 
BspHI T'CATG_A

Cuts at:   3339    3339 
   Size:       4163
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    859    1213    1427    1729    2110     859 
   Size:        354     214     302     381    2912

  Fragments arranged by size: 

                2912     381     354     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2455    2509    3993    4158      50 
   Size:       2405      54    1484     165      55

  Fragments arranged by size: 

                2405    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    549     731     743    1067    2075     549 
   Size:        182      12     324    1008    2637

  Fragments arranged by size: 

                2637    1008     324     182      12
 
BsrFI r'CCGG_y

Cuts at:    627    2161    2342    2551     627 
   Size:       1534     181     209    2239

  Fragments arranged by size: 

                2239    1534     209     181
 
BssSI C'ACGA_G

Cuts at:   2434    2792    2434 
   Size:        358    3805
 
BstAPI GCAn_nnn'nTGC

Cuts at:    264    1540     264 
   Size:       1276    2887
 
BstBI TT'CG_AA

Cuts at:    207    2524     207 
   Size:       2317    1846
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1056    1056 
   Size:       4163
 
BstYI r'GATC_y

Cuts at:      1     536    1188    1655    1678    2013    2259    3271    3357 
   Size:        535     652     467      23     335     246    1012      86

Cuts at:   3357    3369    3715       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     652     535     467     449     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    294     294 
   Size:       4163
 
BtgI C'CryG_G

Cuts at:    107     637     107 
   Size:        530    3633
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2113    2294    2457    2113 
   Size:        181     163    3819

  Fragments arranged by size: 

                3819     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1406    1406 
   Size:       4163
 
ClaI AT'CG_AT

Cuts at:    804     804 
   Size:       4163
 
DraI TTT'AAA

Cuts at:   1162    1162 
   Size:       4163
 
DrdI GACnn_nn'nnGTC

Cuts at:   1869    2727    1869 
   Size:        858    3305
 
EaeI y'GGCC_r

Cuts at:    307    1748    1922    2313    2340    3685     307 
   Size:       1441     174     391      27    1345     785

  Fragments arranged by size: 

                1441    1345     785     391     174      27
 
EagI C'GGCC_G

Cuts at:   1748    1748 
   Size:       4163
 
EarI CTCTTCn'nnn_

Cuts at:    275     515    1106    2186    2396     275 
   Size:        240     591    1080     210    2042

  Fragments arranged by size: 

                2042    1080     591     240     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2371    2681    2827    3973    2371 
   Size:        310     146    1146    2561

  Fragments arranged by size: 

                2561    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1175    1175 
   Size:       4163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    539    1010    1055    1147    1988    2420    3167    3435     539 
   Size:        471      45      92     841     432     747     268    1267

  Fragments arranged by size: 

                1267     841     747     471     432     268      92      45
            
 
EcoO109I rG'GnC_Cy

Cuts at:   1302    1302 
   Size:       4163
 
EcoRI G'AATT_C

Cuts at:   1167    1167 
   Size:       4163
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2182    2182 
   Size:       4163
 
FspI TGC'GCA

Cuts at:   1944    1944 
   Size:       4163
 
HaeII r_GCGC'y

Cuts at:   1597    1845    2867    3846    1597 
   Size:        248    1022     979    1914

  Fragments arranged by size: 

                1914    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    902     934    2005    2035    2037    2067     902 
   Size:         32    1071      30       2      30    2998

  Fragments arranged by size: 

                2998    1071      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1202    1202 
   Size:       4163
 
KasI G'GCGC_C

Cuts at:   1593    1841    1593 
   Size:        248    3915
 
KpnI G_GTAC'C

Cuts at:   1183    1183 
   Size:       4163
 
MluI A'CGCG_T

Cuts at:   3596    3596 
   Size:       4163
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    700     702     957    2834    3018     700 
   Size:          2     255    1877     184    1845

  Fragments arranged by size: 

                1877    1845     255     184       2
 
MscI TGG'CCA

Cuts at:   1924    1924 
   Size:       4163
 
MslI CAynn'nnrTG

Cuts at:    317     356     524     736     752     925    1054    2152    2279 
   Size:         39     168     212      16     173     129    1098     127

Cuts at:   2279     317 
   Size:       2201

  Fragments arranged by size: 

                2201    1098     212     173     168     129     127      39
                  16
 
MspA1I CmG'CkG

Cuts at:    167     382    1274    1588    1948    2961    3206     167 
   Size:        215     892     314     360    1013     245    1124

  Fragments arranged by size: 

                1124    1013     892     360     314     245     215
 
NaeI GCC'GGC

Cuts at:   2344    2344 
   Size:       4163
 
NarI GG'CG_CC

Cuts at:   1594    1842    1594 
   Size:        248    3915
 
NcoI C'CATG_G

Cuts at:    107     637     107 
   Size:        530    3633
 
NgoMIV G'CCGG_C

Cuts at:   2342    2342 
   Size:       4163
 
NspI r_CATG'y

Cuts at:   1216    1716    2623    1216 
   Size:        500     907    2756

  Fragments arranged by size: 

                2756     907     500
 
PciI A'CATG_T

Cuts at:   2619    2619 
   Size:       4163
 
PflMI CCAn_nnn'nTGG

Cuts at:   1033    1033 
   Size:       4163
 
PfoI T'CCnGG_A

Cuts at:    771    1032     771 
   Size:        261    3902
 
PmeI GTTT'AAAC

Cuts at:   1162    1162 
   Size:       4163
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3302    3334    3302 
   Size:         32    4131
 
PstI C_TGCA'G

Cuts at:    155     387     870    1210    1895     155 
   Size:        232     483     340     685    2423

  Fragments arranged by size: 

                2423     685     483     340     232
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4163
 
PvuII CAG'CTG

Cuts at:    167     382    1588    1948     167 
   Size:        215    1206     360    2382

  Fragments arranged by size: 

                2382    1206     360     215
 
RsrII CG'GwC_CG

Cuts at:   2358    2358 
   Size:       4163
 
SacI G_AGCT'C

Cuts at:   1177    1177 
   Size:       4163
 
SalI G'TCGA_C

Cuts at:   1200    1200 
   Size:       4163
 
SapI GCTCTTCn'nnn_

Cuts at:   1106    2186    2396    1106 
   Size:       1080     210    2873

  Fragments arranged by size: 

                2873    1080     210
 
SbfI CC_TGCA'GG

Cuts at:   1210    1210 
   Size:       4163
 
ScaI AGT'ACT

Cuts at:   3680    3680 
   Size:       4163
 
SfcI C'TryA_G

Cuts at:     33     151     383     866    1206    1891    2884    3075      33 
   Size:        118     232     483     340     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     483     340     232     191     118
            
 
SfoI GGC'GCC

Cuts at:   1595    1843    1595 
   Size:        248    3915
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4163
 
SmaI CCC'GGG

Cuts at:   1185    1185 
   Size:       4163
 
SmlI C'TyrA_G

Cuts at:   1318    2233    2725    2987    1318 
   Size:        915     492     262    2494

  Fragments arranged by size: 

                2494     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4163
 
SphI G_CATG'C

Cuts at:   1216    1716    1216 
   Size:        500    3663
 
StyI C'CwwG_G

Cuts at:    107     637     681    1297    1651     107 
   Size:        530      44     616     354    2619

  Fragments arranged by size: 

                2619     616     530     354      44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    276    1746    1879    2338    2547     276 
   Size:       1470     133     459     209    1892

  Fragments arranged by size: 

                1892    1470     459     209     133
 
TatI w'GTAC_w

Cuts at:   3678    3678 
   Size:       4163
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    154     186    1014    1046    3680    3712     154 
   Size:         32     828      32    2634      32     605

  Fragments arranged by size: 

                2634     828     605      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1960    1960 
   Size:       4163
 
XbaI T'CTAG_A

Cuts at:   1194    1194 
   Size:       4163
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    644     969    1658     644 
   Size:        325     689    3149

  Fragments arranged by size: 

                3149     689     325
 
XmaI C'CCGG_G

Cuts at:   1183    1183 
   Size:       4163
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AloI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI
     PfoI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     ApaI     AscI
     BaeI     BbsI    BfrBI     BglI    BmgBI     BmtI    BseRI    BsiWI
    BsmBI    BsrGI   BssHII   BstEII  BstZ17I    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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