Restriction map for pF1KB3480
 (Circular) (Six-base) MAPSORT of: pF1KB3480.seq  Check: 5525  from: 1  to: 4061



pF1KB3480  4061 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AatII G_ACGT'C

Cuts at:    897     897 
   Size:       4061
 
AccI GT'mk_AC

Cuts at:   1099    1099 
   Size:       4061
 
Acc65I G'GTAC_C

Cuts at:   1077    1077 
   Size:       4061
 
AclI AA'CG_TT

Cuts at:   1055    3901    1055 
   Size:       2846    1215
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1886    2318    3065    3333    1886 
   Size:        432     747     268    2614

  Fragments arranged by size: 

                2614     747     432     268
 
AflIII A'CryG_T

Cuts at:    410    1413    2517    3494     410 
   Size:       1003    1104     977     977

  Fragments arranged by size: 

                1104    1003     977     977
 
AgeI A'CCGG_T

Cuts at:   2449    2449 
   Size:       4061
 
AleI CACnn'nnGTG

Cuts at:    650     650 
   Size:       4061
 
AlwNI CAG_nnn'CTG

Cuts at:   1366    2933    1366 
   Size:       1567    2494
 
ApaLI G'TGCA_C

Cuts at:   2831    2831 
   Size:       4061
 
ApoI r'AATT_y

Cuts at:    595    1065    3414    3670     595 
   Size:        470    2349     256     986

  Fragments arranged by size: 

                2349     986     470     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4061
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4061
 
AvaI C'yCGr_G

Cuts at:   1081    1081 
   Size:       4061
 
BamHI G'GATC_C

Cuts at:    609    1086     609 
   Size:        477    3584
 
BanI G'GyrC_C

Cuts at:    530     987    1077    1491    1739    1774     530 
   Size:        457      90     414     248      35    2817

  Fragments arranged by size: 

                2817     457     414     248      90      35
 
BanII G_rGCy'C

Cuts at:    245     300    1075    2105     245 
   Size:         55     775    1030    2201

  Fragments arranged by size: 

                2201    1030     775      55
 
BbeI G_GCGC'C

Cuts at:   1495    1743    1495 
   Size:        248    3813
 
BbvCI CC'TCA_GC

Cuts at:    436     526     436 
   Size:         90    3971
 
BciVI GTATCCnnnnn_n'

Cuts at:   1959    2720    4061    1959 
   Size:        761    1341    1959

  Fragments arranged by size: 

                1959    1341     761
 
BclI T'GATC_A

Cuts at:   1581    1581 
   Size:       4061
 
BglI GCCn_nnn'nGGC

Cuts at:    422     422 
   Size:       4061
 
BglII A'GATC_T

Cuts at:   1576    3613    1576 
   Size:       2037    2024
 
BlpI GC'TnA_GC

Cuts at:   1173    1173 
   Size:       4061
 
Bme1580I G_kGCm'C

Cuts at:    728     990    1686    1779    2835     728 
   Size:        262     696      93    1056    1954

  Fragments arranged by size: 

                1954    1056     696     262      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1273    1354    1689    3833    1273 
   Size:         81     335    2144    1501

  Fragments arranged by size: 

                2144    1501     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    333    2428     333 
   Size:       2095    1966
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    247     466     517     247 
   Size:        219      51    3791

  Fragments arranged by size: 

                3791     219      51
 
Bpu10I CC'TnA_GC

Cuts at:    436     526     436 
   Size:         90    3971
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    259    1237    2116    2608    2906     259 
   Size:        978     879     492     298    1414

  Fragments arranged by size: 

                1414     978     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    112    3765     112 
   Size:       3653     408
 
BsaAI yAC'GTr

Cuts at:    413    1414     413 
   Size:       1001    3060
 
BsaBI GATnn'nnATC

Cuts at:      6     907    1575    3612       6 
   Size:        901     668    2037     455

  Fragments arranged by size: 

                2037     901     668     455
 
BsaHI Gr'CG_yC

Cuts at:    894    1492    1740    2442    3938     894 
   Size:        598     248     702    1496    1017

  Fragments arranged by size: 

                1496    1017     702     598     248
 
BsaWI w'CCGG_w

Cuts at:   1250    1771    2449    2723    2870    3281    1250 
   Size:        521     678     274     147     411    2030

  Fragments arranged by size: 

                2030     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    397     427    1011    1041     397 
   Size:         30     584      30    3417

  Fragments arranged by size: 

                3417     584      30      30
 
BseYI C'CCAG_C

Cuts at:    367     727    1486    2244    2821    3317     367 
   Size:        360     759     758     577     496    1111

  Fragments arranged by size: 

                1111     759     758     577     496     360
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    648     648 
   Size:       4061
 
BsiEI CG_ry'CG

Cuts at:    104    1603    1649    2857     104 
   Size:       1499      46    1208    1308

  Fragments arranged by size: 

                1499    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    263     482    1075    1853    2043    2835     263 
   Size:        219     593     778     190     792    1489

  Fragments arranged by size: 

                1489     792     778     593     219     190
 
Bsp1286I G_dGCh'C

Cuts at:    245     263     300     482     728     990    1075    1686    1779 
   Size:         18      37     182     246     262      85     611      93

Cuts at:   1779    1853    2043    2105    2835     245 
   Size:         74     190      62     730    1471

  Fragments arranged by size: 

                1471     730     611     262     246     190     182      93
                  85      74      62      37      18
 
BspEI T'CCGG_A

Cuts at:   3281    3281 
   Size:       4061
 
BspHI T'CATG_A

Cuts at:   3237    3237 
   Size:       4061
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1111    1325    1627    2008    1111 
   Size:        214     302     381    3164

  Fragments arranged by size: 

                3164     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2353    2407    3891    4056      50 
   Size:       2303      54    1484     165      55

  Fragments arranged by size: 

                2303    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    773    1973     773 
   Size:       1200    2861
 
BsrFI r'CCGG_y

Cuts at:   2059    2240    2449    2059 
   Size:        181     209    3671

  Fragments arranged by size: 

                3671     209     181
 
BssSI C'ACGA_G

Cuts at:   2332    2690    2332 
   Size:        358    3703
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1438    1438 
   Size:       4061
 
BstBI TT'CG_AA

Cuts at:   2422    2422 
   Size:       4061
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     114 
   Size:       4061
 
BstYI r'GATC_y

Cuts at:      1     609    1086    1553    1576    1911    2157    3169    3255 
   Size:        608     477     467      23     335     246    1012      86

Cuts at:   3255    3267    3613       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     608     477     467     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    247     247 
   Size:       4061
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2011    2192    2355    2011 
   Size:        181     163    3717

  Fragments arranged by size: 

                3717     181     163
 
BtsI GCAGTG_nn'

Cuts at:    660    1304     660 
   Size:        644    3417
 
DraI TTT'AAA

Cuts at:   1060    1060 
   Size:       4061
 
DrdI GACnn_nn'nnGTC

Cuts at:    126    1767    2625     126 
   Size:       1641     858    1562

  Fragments arranged by size: 

                1641    1562     858
 
EaeI y'GGCC_r

Cuts at:    423    1646    1820    2211    2238    3583     423 
   Size:       1223     174     391      27    1345     901

  Fragments arranged by size: 

                1345    1223     901     391     174      27
 
EagI C'GGCC_G

Cuts at:   1646    1646 
   Size:       4061
 
EarI CTCTTCn'nnn_

Cuts at:    747    1028    2084    2294     747 
   Size:        281    1056     210    2514

  Fragments arranged by size: 

                2514    1056     281     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2269    2579    2725    3871    2269 
   Size:        310     146    1146    2459

  Fragments arranged by size: 

                2459    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1073    1073 
   Size:       4061
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    247     466     517    1886    2318    3065    3333     247 
   Size:        219      51    1369     432     747     268     975

  Fragments arranged by size: 

                1369     975     747     432     268     219      51
 
EcoO109I rG'GnC_Cy

Cuts at:   1200    1200 
   Size:       4061
 
EcoRI G'AATT_C

Cuts at:   1065    1065 
   Size:       4061
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2080    2080 
   Size:       4061
 
FspI TGC'GCA

Cuts at:   1842    1842 
   Size:       4061
 
HaeII r_GCGC'y

Cuts at:   1495    1743    2765    3744    1495 
   Size:        248    1022     979    1812

  Fragments arranged by size: 

                1812    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    503     535    1011    1043    1903    1933    1935    1965     503 
   Size:         32     476      32     860      30       2      30    2599

  Fragments arranged by size: 

                2599     860     476      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1044    1100    1044 
   Size:         56    4005
 
KasI G'GCGC_C

Cuts at:   1491    1739    1491 
   Size:        248    3813
 
KpnI G_GTAC'C

Cuts at:   1081    1081 
   Size:       4061
 
MluI A'CGCG_T

Cuts at:   3494    3494 
   Size:       4061
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2732    2916    2732 
   Size:        184    3877
 
MscI TGG'CCA

Cuts at:    425    1822     425 
   Size:       1397    2664
 
MslI CAynn'nnrTG

Cuts at:    112     650    2050    2177     112 
   Size:        538    1400     127    1996

  Fragments arranged by size: 

                1996    1400     538     127
 
MspA1I CmG'CkG

Cuts at:    401     539     955    1172    1486    1846    2859    3104     401 
   Size:        138     416     217     314     360    1013     245    1358

  Fragments arranged by size: 

                1358    1013     416     360     314     245     217     138
            
 
NaeI GCC'GGC

Cuts at:   2242    2242 
   Size:       4061
 
NarI GG'CG_CC

Cuts at:   1492    1740    1492 
   Size:        248    3813
 
NgoMIV G'CCGG_C

Cuts at:   2240    2240 
   Size:       4061
 
NspI r_CATG'y

Cuts at:    290    1114    1614    2521     290 
   Size:        824     500     907    1830

  Fragments arranged by size: 

                1830     907     824     500
 
PasI CC'CwG_GG

Cuts at:    983     983 
   Size:       4061
 
PciI A'CATG_T

Cuts at:   2517    2517 
   Size:       4061
 
PflMI CCAn_nnn'nTGG

Cuts at:    326     326 
   Size:       4061
 
PfoI T'CCnGG_A

Cuts at:    263     503     263 
   Size:        240    3821
 
PmeI GTTT'AAAC

Cuts at:   1060    1060 
   Size:       4061
 
PmlI CAC'GTG

Cuts at:    413     413 
   Size:       4061
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3200    3232    3200 
   Size:         32    4029
 
PstI C_TGCA'G

Cuts at:    201     829    1108    1793     201 
   Size:        628     279     685    2469

  Fragments arranged by size: 

                2469     685     628     279
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4061
 
PvuII CAG'CTG

Cuts at:    539     955    1486    1846     539 
   Size:        416     531     360    2754

  Fragments arranged by size: 

                2754     531     416     360
 
RsrII CG'GwC_CG

Cuts at:   2256    2256 
   Size:       4061
 
SacI G_AGCT'C

Cuts at:   1075    1075 
   Size:       4061
 
SalI G'TCGA_C

Cuts at:   1098    1098 
   Size:       4061
 
SapI GCTCTTCn'nnn_

Cuts at:    747    2084    2294     747 
   Size:       1337     210    2514

  Fragments arranged by size: 

                2514    1337     210
 
SbfI CC_TGCA'GG

Cuts at:   1108    1108 
   Size:       4061
 
ScaI AGT'ACT

Cuts at:   3578    3578 
   Size:       4061
 
SfcI C'TryA_G

Cuts at:     33     197     372     785     806     825    1104    1789    2782 
   Size:        164     175     413      21      19     279     685     993

Cuts at:   2782    2973      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     685     413     279     191     175     164
                  21      19
 
SfoI GGC'GCC

Cuts at:   1493    1741    1493 
   Size:        248    3813
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4061
 
SmaI CCC'GGG

Cuts at:   1083    1083 
   Size:       4061
 
SmlI C'TyrA_G

Cuts at:    238    1216    2131    2623    2885     238 
   Size:        978     915     492     262    1414

  Fragments arranged by size: 

                1414     978     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4061
 
SphI G_CATG'C

Cuts at:   1114    1614    1114 
   Size:        500    3561
 
StyI C'CwwG_G

Cuts at:   1195    1549    1195 
   Size:        354    3707
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    370    1644    1777    2236    2445     370 
   Size:       1274     133     459     209    1986

  Fragments arranged by size: 

                1986    1274     459     209     133
 
TatI w'GTAC_w

Cuts at:    407    3576     407 
   Size:       3169     892
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    397     429    3578    3610     397 
   Size:         32    3149      32     848

  Fragments arranged by size: 

                3149     848      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1039    1858    1039 
   Size:        819    3242
 
XbaI T'CTAG_A

Cuts at:   1092    1092 
   Size:       4061
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    646    1556     646 
   Size:        910    3151
 
XmaI C'CCGG_G

Cuts at:   1081    1081 
   Size:       4061
 
ZraI GAC'GTC

Cuts at:    895     895 
   Size:       4061
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI     BcgI    BfrBI    BmgBI     BmtI    BseRI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI
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