Restriction map for pF1KB3440
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       BalI        BamHI       BanI        
BbeI        BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlpI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DdeI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MluNI       
MroI        MscI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          589  GTCTAC               GT^[AC][GT]AC
AccII         135  CGCG                 CG^CG
AfaI          258  GTAC                 GT^AC
AflIII        134  ACGCGT               A^C[GA][CT]GT
AlwNI         547  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       152  AGCGCT               AGC^GCT
AspHI         607  GAGCAC               G[AT]GC[AT]^C
AvrII         370  CCTAGG               C^CTAGG
BbrPI         433  CACGTG               CAC^GTG
BfaI          371  CTAG                 C^TAG
BlnI          370  CCTAGG               C^CTAGG
BsaAI         433  CACGTG               [CT]AC^GT[GA]
BsgI          315  GTGCAG               GTGCAG(16/14)
BsiHKAI       607  GAGCAC               G[AT]GC[AT]^C
BsmAI         533  GAGAC                GTCTC(1/5)
BstUI         135  CGCG                 CG^CG
DsaI          635  CCGTGG               C^C[GA][CT]GG
Eco47III      152  AGCGCT               AGC^GCT
EcoNI         108  CCTTTTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       370  CCTAGG               C^C[AT][AT]GG
MaeI          371  CTAG                 C^TAG
MboII         731  TCTTC                GAAGA(8/7)
MluI          134  ACGCGT               A^CGCGT
MseI          140  TTAA                 T^TAA
MspA1I        497  CAGCTG               C[AC]G^C[GT]G
MvnI          135  CGCG                 CG^CG
PflMI         546  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          362  GAGTC                GAGTC(4/5)
PmaCI         433  CACGTG               CAC^GTG
PmlI          433  CACGTG               CAC^GTG
PvuII         497  CAGCTG               CAG^CTG
RsaI          258  GTAC                 GT^AC
ScaI          257  AGTACT               AGT^ACT
StyI          370  CCTAGG               C^C[AT][AT]GG
TaqI          200  TCGA                 T^CGA
TfiI          658  GATTC                G^A[AT]TC
Tru9I         140  TTAA                 T^TAA
Tsp509I       103  AATT                 ^AATT
TthHB8I       200  TCGA                 T^CGA
Van91I        546  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

ApaI          340  GGGCCC               GGGCC^C
              400  GGGCCC              
AvaII          78  GGTCC                G^G[AT]CC
              539  GGACC               
BsmFI         117  GGGAC                GGGAC(10/14)
              145  GGGAC               
BsmI          127  GAATGC               GAATGC(1/-1)
              458  GCATTC              
BsrI          260  ACTGG                ACTGG(1/-1)
              536  ACTGG               
BstF5I        279  CATCC                GGATG(2/0)
              311  GGATG               
BstYI         548  AGATCC               [GA]^GATC[CT]
              766  GGATCT              
Cac8I         294  GCGGGC               GC[ACGT]^[ACGT]GC
              338  GCGGGC              
DraII         399  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              400  GGGCCCT             
EcoO109I      399  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              400  GGGCCCT             
FauI          294  GCGGG                CCCGC(4/6)
              338  GCGGG               
FokI          279  CATCC                GGATG(9/13)
              311  GGATG               
HaeII         152  AGCGCT               [GA]GCGC^[CT]
              356  AGCGCC              
HinfI         362  GAGTC                G^A[ACGT]TC
              658  GATTC               
MaeII         209  ACGT                 A^CGT
              434  ACGT                
MflI          548  AGATCC               [GA]^GATC[CT]
              766  GGATCT              
MslI           69  CACATCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              729  CATCTTCATG          
NlaIII        345  CATG                 CATG^
              735  CATG                
PstI           10  CTGCAG               CTGCA^G
              352  CTGCAG              
SfaNI         558  GATGC                GCATC(5/9)
              728  GCATC               
SfcI           10  CTGCAG               C^T[GA][CT]AG
              352  CTGCAG              
TspRI          35  CAGTG                CA[GC]TG[ACGT][ACGT]^
              299  CAGTG               
XhoII         548  AGATCC               [GA]^GATC[CT]
              766  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AluI        AlwI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        Bsp1286I    BspMI       BssKI       
BstNI       CfoI        Cfr13I      DpnII       EcoRII      
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MaeIII      MboI        
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIV       Sau3AI      Sau96I      ScrFI       
TseI        Tsp45I      
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