Restriction map for pF1KB3428
 (Linear) (Six-base) MAPSORT of: KIBB3428.seq  Check: 8368  from: 1  to: 1269



KIBB3428.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     556     805    1237    1269 
   Size:        556     249     432      32

  Fragments arranged by size: 

                 556     432     249      32
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      62     689    1269 
   Size:         62     627     580

  Fragments arranged by size: 

                 627     580      62
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      48      80    1269 
   Size:         48      32    1189

  Fragments arranged by size: 

                1189      48      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     507    1269 
   Size:        507     762
 
AvaI C'yCGr_G

Cuts at:      0     164    1269 
   Size:        164    1105
 
BanI G'GyrC_C

Cuts at:      0     489    1269 
   Size:        489     780
 
BanII G_rGCy'C

Cuts at:      0     250    1269 
   Size:        250    1019
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     211     998    1051    1269 
   Size:        211     787      53     218

  Fragments arranged by size: 

                 787     218     211      53
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1221    1255    1269 
   Size:       1221      34      14

  Fragments arranged by size: 

                1221      34      14
 
BlpI GC'TnA_GC

Cuts at:      0     934    1269 
   Size:        934     335
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1227    1269 
   Size:       1227      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     829     936    1269 
   Size:        352     477     107     333

  Fragments arranged by size: 

                 477     352     333     107
 
Bpu10I CC'TnA_GC

Cuts at:      0     211    1269 
   Size:        211    1058
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     787     817     833     863    1269 
   Size:        787      30      16      30     406

  Fragments arranged by size: 

                 787     406      30      30      16
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     478     564    1269 
   Size:        478      86     705

  Fragments arranged by size: 

                 705     478      86
 
BseYI C'CCAG_C

Cuts at:      0     662    1269 
   Size:        662     607
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     346     706    1269 
   Size:         58     288     360     563

  Fragments arranged by size: 

                 563     360     288      58
 
BsiHKAI G_wGCw'C

Cuts at:      0     302    1187    1269 
   Size:        302     885      82

  Fragments arranged by size: 

                 885     302      82
 
Bsp1286I G_dGCh'C

Cuts at:      0     250     302    1187    1269 
   Size:        250      52     885      82

  Fragments arranged by size: 

                 885     250      82      52
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     287     782    1247    1269 
   Size:        287     495     465      22

  Fragments arranged by size: 

                 495     465     287      22
 
BsrBI CCG'CTC

Cuts at:      0       7    1269 
   Size:          7    1262
 
BsrDI GCAATG_nn'

Cuts at:      0     416    1269 
   Size:        416     853
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     807    1269 
   Size:        807     462
 
BstYI r'GATC_y

Cuts at:      0     179     656    1269 
   Size:        179     477     613

  Fragments arranged by size: 

                 613     477     179
 
BtsI GCAGTG_nn'

Cuts at:      0     105    1269 
   Size:        105    1164
 
EaeI y'GGCC_r

Cuts at:      0       2      92     413     454    1269 
   Size:          2      90     321      41     815

  Fragments arranged by size: 

                 815     321      90      41       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     295     601    1192    1269 
   Size:        295     306     591      77

  Fragments arranged by size: 

                 591     306     295      77
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     556     805     829     936    1237    1269 
   Size:        352     204     249      24     107     301      32

  Fragments arranged by size: 

                 352     301     249     204     107      32      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1053    1236    1269 
   Size:       1053     183      33

  Fragments arranged by size: 

                1053     183      33
 
EcoRV GAT'ATC

Cuts at:      0     744    1269 
   Size:        744     525
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     282    1269 
   Size:        282     987
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     141     173    1269 
   Size:        141      32    1096

  Fragments arranged by size: 

                1096     141      32
 
HindIII A'AGCT_T

Cuts at:      0    1219    1269 
   Size:       1219      50
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      92    1269 
   Size:         92    1177
 
MscI TGG'CCA

Cuts at:      0      94     415    1269 
   Size:         94     321     854

  Fragments arranged by size: 

                 854     321      94
 
MslI CAynn'nnrTG

Cuts at:      0     910    1039    1269 
   Size:        910     129     230

  Fragments arranged by size: 

                 910     230     129
 
MspA1I CmG'CkG

Cuts at:      0     720    1269 
   Size:        720     549
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     567    1269 
   Size:        567     702
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     487     519    1269 
   Size:        487      32     750

  Fragments arranged by size: 

                 750     487      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1053    1236    1269 
   Size:       1053     183      33

  Fragments arranged by size: 

                1053     183      33
 
PshAI GACnn'nnGTC

Cuts at:      0    1051    1269 
   Size:       1051     218
 
PstI C_TGCA'G

Cuts at:      0     584    1269 
   Size:        584     685
 
PvuII CAG'CTG

Cuts at:      0     720    1269 
   Size:        720     549
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1192    1269 
   Size:       1192      77
 
SfcI C'TryA_G

Cuts at:      0     580     715    1269 
   Size:        580     135     554

  Fragments arranged by size: 

                 580     554     135
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     912    1158    1269 
   Size:        912     246     111

  Fragments arranged by size: 

                 912     246     111
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     833     865    1269 
   Size:        833      32     404

  Fragments arranged by size: 

                 833     404      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      75     442    1269 
   Size:         75     367     827

  Fragments arranged by size: 

                 827     367      75
 

 Enzymes that do cut:

     AcuI     AlfI     AloI    AlwNI     AvaI     BanI    BanII     BbsI
     BcgI     BlpI     BplI     BpmI   Bpu10I    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BstXI    BstYI
     BtsI     EaeI     EarI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I
  HindIII     MmeI     MscI     MslI   MspA1I    PflMI     PpiI    PpuMI
    PshAI     PstI    PvuII     SapI     SfcI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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