(Circular) (Six-base) MAPSORT of: pF1KB3416.seq Check: 9463 from: 1 to: 3524 pF1KB3416 3524 bp With 182 enzymes: SgfI * April 24, 2009 13:01 .. AccI GT'mk_AC Cuts at: 562 562 Size: 3524 Acc65I G'GTAC_C Cuts at: 540 540 Size: 3524 AclI AA'CG_TT Cuts at: 3364 3364 Size: 3524 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1349 1781 2528 2796 1349 Size: 432 747 268 2077 Fragments arranged by size: 2077 747 432 268 AflIII A'CryG_T Cuts at: 876 1980 2957 876 Size: 1104 977 1443 Fragments arranged by size: 1443 1104 977 AgeI A'CCGG_T Cuts at: 1912 1912 Size: 3524 AlwNI CAG_nnn'CTG Cuts at: 829 2396 829 Size: 1567 1957 ApaI G_GGCC'C Cuts at: 351 351 Size: 3524 ApaLI G'TGCA_C Cuts at: 2294 2294 Size: 3524 ApoI r'AATT_y Cuts at: 528 2877 3133 528 Size: 2349 256 919 Fragments arranged by size: 2349 919 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3524 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3524 AvaI C'yCGr_G Cuts at: 544 544 Size: 3524 BamHI G'GATC_C Cuts at: 323 549 323 Size: 226 3298 BanI G'GyrC_C Cuts at: 540 954 1202 1237 540 Size: 414 248 35 2827 Fragments arranged by size: 2827 414 248 35 BanII G_rGCy'C Cuts at: 265 351 538 1568 265 Size: 86 187 1030 2221 Fragments arranged by size: 2221 1030 187 86 BbeI G_GCGC'C Cuts at: 958 1206 958 Size: 248 3276 BciVI GTATCCnnnnn_n' Cuts at: 1422 2183 3524 1422 Size: 761 1341 1422 Fragments arranged by size: 1422 1341 761 BclI T'GATC_A Cuts at: 416 1044 416 Size: 628 2896 BglII A'GATC_T Cuts at: 1039 3076 1039 Size: 2037 1487 BlpI GC'TnA_GC Cuts at: 636 636 Size: 3524 Bme1580I G_kGCm'C Cuts at: 351 453 1149 1242 2298 351 Size: 102 696 93 1056 1577 Fragments arranged by size: 1577 1056 696 102 93 BmrI ACTGGGnnnn_n' Cuts at: 736 817 1152 3296 736 Size: 81 335 2144 964 Fragments arranged by size: 2144 964 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 316 1891 316 Size: 1575 1949 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 700 1579 2071 2369 700 Size: 879 492 298 1855 Fragments arranged by size: 1855 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3228 3228 Size: 3524 BsaAI yAC'GTr Cuts at: 454 877 454 Size: 423 3101 BsaBI GATnn'nnATC Cuts at: 6 1038 3075 6 Size: 1032 2037 455 Fragments arranged by size: 2037 1032 455 BsaHI Gr'CG_yC Cuts at: 955 1203 1905 3401 955 Size: 248 702 1496 1078 Fragments arranged by size: 1496 1078 702 248 BsaWI w'CCGG_w Cuts at: 713 1234 1912 2186 2333 2744 713 Size: 521 678 274 147 411 1493 Fragments arranged by size: 1493 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 118 118 Size: 3524 BseYI C'CCAG_C Cuts at: 258 949 1707 2284 2780 258 Size: 691 758 577 496 1002 Fragments arranged by size: 1002 758 691 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 168 168 Size: 3524 BsiEI CG_ry'CG Cuts at: 104 196 1066 1112 2320 104 Size: 92 870 46 1208 1308 Fragments arranged by size: 1308 1208 870 92 46 BsiHKAI G_wGCw'C Cuts at: 538 1316 1506 2298 538 Size: 778 190 792 1764 Fragments arranged by size: 1764 792 778 190 Bsp1286I G_dGCh'C Cuts at: 265 351 453 538 1149 1242 1316 1506 1568 Size: 86 102 85 611 93 74 190 62 Cuts at: 1568 2298 265 Size: 730 1491 Fragments arranged by size: 1491 730 611 190 102 93 86 85 74 62 BspEI T'CCGG_A Cuts at: 2744 2744 Size: 3524 BspHI T'CATG_A Cuts at: 2700 2700 Size: 3524 BspMI ACCTGCnnnn'nnnn_ Cuts at: 574 788 1090 1471 574 Size: 214 302 381 2627 Fragments arranged by size: 2627 381 302 214 BsrBI CCG'CTC Cuts at: 50 1816 1870 3354 3519 50 Size: 1766 54 1484 165 55 Fragments arranged by size: 1766 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 274 1436 274 Size: 1162 2362 BsrFI r'CCGG_y Cuts at: 1522 1703 1912 1522 Size: 181 209 3134 Fragments arranged by size: 3134 209 181 BssSI C'ACGA_G Cuts at: 1795 2153 1795 Size: 358 3166 BstAPI GCAn_nnn'nTGC Cuts at: 901 901 Size: 3524 BstBI TT'CG_AA Cuts at: 1885 1885 Size: 3524 BstYI r'GATC_y Cuts at: 1 323 549 1016 1039 1374 1620 2632 2718 Size: 322 226 467 23 335 246 1012 86 Cuts at: 2718 2730 3076 1 Size: 12 346 449 Fragments arranged by size: 1012 467 449 346 335 322 246 226 86 23 12 BtgI C'CryG_G Cuts at: 107 144 107 Size: 37 3487 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1474 1655 1818 1474 Size: 181 163 3180 Fragments arranged by size: 3180 181 163 BtsI GCAGTG_nn' Cuts at: 767 767 Size: 3524 DraI TTT'AAA Cuts at: 523 523 Size: 3524 DrdI GACnn_nn'nnGTC Cuts at: 1230 2088 1230 Size: 858 2666 EaeI y'GGCC_r Cuts at: 193 240 1109 1283 1674 1701 3046 193 Size: 47 869 174 391 27 1345 671 Fragments arranged by size: 1345 869 671 391 174 47 27 EagI C'GGCC_G Cuts at: 193 1109 193 Size: 916 2608 EarI CTCTTCn'nnn_ Cuts at: 1547 1757 1547 Size: 210 3314 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 154 1732 2042 2188 3334 154 Size: 1578 310 146 1146 344 Fragments arranged by size: 1578 1146 344 310 146 EcoICRI GAG'CTC Cuts at: 536 536 Size: 3524 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1349 1781 2528 2796 1349 Size: 432 747 268 2077 Fragments arranged by size: 2077 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 133 133 Size: 3524 EcoO109I rG'GnC_Cy Cuts at: 347 663 347 Size: 316 3208 EcoRI G'AATT_C Cuts at: 528 528 Size: 3524 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1543 1543 Size: 3524 FspI TGC'GCA Cuts at: 1305 1305 Size: 3524 HaeII r_GCGC'y Cuts at: 958 1206 2228 3207 958 Size: 248 1022 979 1275 Fragments arranged by size: 1275 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1366 1396 1398 1428 1366 Size: 30 2 30 3462 Fragments arranged by size: 3462 30 30 2 HincII GTy'rAC Cuts at: 563 563 Size: 3524 HindIII A'AGCT_T Cuts at: 373 373 Size: 3524 KasI G'GCGC_C Cuts at: 954 1202 954 Size: 248 3276 KpnI G_GTAC'C Cuts at: 544 544 Size: 3524 MluI A'CGCG_T Cuts at: 2957 2957 Size: 3524 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2195 2379 2195 Size: 184 3340 MscI TGG'CCA Cuts at: 1285 1285 Size: 3524 MslI CAynn'nnrTG Cuts at: 1513 1640 1513 Size: 127 3397 MspA1I CmG'CkG Cuts at: 258 635 949 1309 2322 2567 258 Size: 377 314 360 1013 245 1215 Fragments arranged by size: 1215 1013 377 360 314 245 NaeI GCC'GGC Cuts at: 1705 1705 Size: 3524 NarI GG'CG_CC Cuts at: 955 1203 955 Size: 248 3276 NcoI C'CATG_G Cuts at: 107 107 Size: 3524 NgoMIV G'CCGG_C Cuts at: 1703 1703 Size: 3524 NspI r_CATG'y Cuts at: 577 1077 1984 577 Size: 500 907 2117 Fragments arranged by size: 2117 907 500 PasI CC'CwG_GG Cuts at: 174 233 174 Size: 59 3465 PciI A'CATG_T Cuts at: 1980 1980 Size: 3524 PmeI GTTT'AAAC Cuts at: 523 523 Size: 3524 PmlI CAC'GTG Cuts at: 454 454 Size: 3524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2663 2695 2663 Size: 32 3492 PspOMI G'GGCC_C Cuts at: 347 347 Size: 3524 PstI C_TGCA'G Cuts at: 571 1256 571 Size: 685 2839 PvuI CG_AT'CG Cuts at: 104 104 Size: 3524 PvuII CAG'CTG Cuts at: 258 949 1309 258 Size: 691 360 2473 Fragments arranged by size: 2473 691 360 RsrII CG'GwC_CG Cuts at: 1719 1719 Size: 3524 SacI G_AGCT'C Cuts at: 538 538 Size: 3524 SalI G'TCGA_C Cuts at: 561 561 Size: 3524 SapI GCTCTTCn'nnn_ Cuts at: 1547 1757 1547 Size: 210 3314 SbfI CC_TGCA'GG Cuts at: 571 571 Size: 3524 ScaI AGT'ACT Cuts at: 3041 3041 Size: 3524 SfcI C'TryA_G Cuts at: 33 494 509 567 1252 2245 2436 33 Size: 461 15 58 685 993 191 1121 Fragments arranged by size: 1121 993 685 461 191 58 15 SfoI GGC'GCC Cuts at: 956 1204 956 Size: 248 3276 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3524 SmaI CCC'GGG Cuts at: 546 546 Size: 3524 SmlI C'TyrA_G Cuts at: 679 1594 2086 2348 679 Size: 915 492 262 1855 Fragments arranged by size: 1855 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3524 SphI G_CATG'C Cuts at: 577 1077 577 Size: 500 3024 StuI AGG'CCT Cuts at: 139 139 Size: 3524 StyI C'CwwG_G Cuts at: 107 658 1012 107 Size: 551 354 2619 Fragments arranged by size: 2619 551 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 166 1107 1240 1699 1908 166 Size: 941 133 459 209 1782 Fragments arranged by size: 1782 941 459 209 133 TatI w'GTAC_w Cuts at: 3039 3039 Size: 3524 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 443 475 3041 3073 443 Size: 32 2566 32 894 Fragments arranged by size: 2566 894 32 32 Tth111I GACn'n_nGTC Cuts at: 1321 1321 Size: 3524 XbaI T'CTAG_A Cuts at: 555 555 Size: 3524 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1019 1019 Size: 3524 XmaI C'CCGG_G Cuts at: 544 544 Size: 3524 XmnI GAAnn'nnTTC Cuts at: 374 374 Size: 3524 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI