Restriction map for pF1KB3393
 (Linear) (Six-base) MAPSORT of: KIBB3393.seq  Check: 190  from: 1  to: 924



KIBB3393.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     567     924 
   Size:        567     357
 
Acc65I G'GTAC_C

Cuts at:      0     128     924 
   Size:        128     796
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     889     924 
   Size:        196     693      35

  Fragments arranged by size: 

                 693     196      35
 
AhdI GACnn_n'nnGTC

Cuts at:      0     357     788     924 
   Size:        357     431     136

  Fragments arranged by size: 

                 431     357     136
 
AlwNI CAG_nnn'CTG

Cuts at:      0     201     924 
   Size:        201     723
 
ApaI G_GGCC'C

Cuts at:      0     249     293     924 
   Size:        249      44     631

  Fragments arranged by size: 

                 631     249      44
 
ApaLI G'TGCA_C

Cuts at:      0     379     442     924 
   Size:        379      63     482

  Fragments arranged by size: 

                 482     379      63
 
BanI G'GyrC_C

Cuts at:      0     128     211     924 
   Size:        128      83     713

  Fragments arranged by size: 

                 713     128      83
 
BanII G_rGCy'C

Cuts at:      0      74     249     293     573     924 
   Size:         74     175      44     280     351

  Fragments arranged by size: 

                 351     280     175      74      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     261     924 
   Size:        261     663
 
BglI GCCn_nnn'nGGC

Cuts at:      0     617     924 
   Size:        617     307
 
BlpI GC'TnA_GC

Cuts at:      0     572     924 
   Size:        572     352
 
Bme1580I G_kGCm'C

Cuts at:      0     214     249     293     329     383     446     924 
   Size:        214      35      44      36      54      63     478

  Fragments arranged by size: 

                 478     214      63      54      44      36      35
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     513     790     924 
   Size:        513     277     134

  Fragments arranged by size: 

                 513     277     134
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     924 
   Size:        448     476
 
BsaAI yAC'GTr

Cuts at:      0     710     924 
   Size:        710     214
 
BsaBI GATnn'nnATC

Cuts at:      0     835     924 
   Size:        835      89
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      34      64     436     466     924 
   Size:         34      30     372      30     458

  Fragments arranged by size: 

                 458     372      34      30      30
 
BseYI C'CCAG_C

Cuts at:      0     257     661     924 
   Size:        257     404     263

  Fragments arranged by size: 

                 404     263     257
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     477     924 
   Size:        477     447
 
BsiHKAI G_wGCw'C

Cuts at:      0     383     446     573     924 
   Size:        383      63     127     351

  Fragments arranged by size: 

                 383     351     127      63
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     424     924 
   Size:        424     500
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     214     249     293     329     383     446     573 
   Size:         74     140      35      44      36      54      63     127

Cuts at:    573     924 
   Size:        351

  Fragments arranged by size: 

                 351     140     127      74      63      54      44      36
                  35
 
BspHI T'CATG_A

Cuts at:      0     779     924 
   Size:        779     145
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     314     567     659     924 
   Size:        314     253      92     265

  Fragments arranged by size: 

                 314     265     253      92
 
BsrDI GCAATG_nn'

Cuts at:      0      83     924 
   Size:         83     841
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     201     924 
   Size:        201     723
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     636     924 
   Size:        636     288
 
BstYI r'GATC_y

Cuts at:      0     680     924 
   Size:        680     244
 
BtgI C'CryG_G

Cuts at:      0     206     215     456     924 
   Size:        206       9     241     468

  Fragments arranged by size: 

                 468     241     206       9
 
BtsI GCAGTG_nn'

Cuts at:      0     130     793     924 
   Size:        130     663     131

  Fragments arranged by size: 

                 663     131     130
 
EaeI y'GGCC_r

Cuts at:      0     218     339     924 
   Size:        218     121     585

  Fragments arranged by size: 

                 585     218     121
 
EarI CTCTTCn'nnn_

Cuts at:      0     109     316     442     721     924 
   Size:        109     207     126     279     203

  Fragments arranged by size: 

                 279     207     203     126     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     139     924 
   Size:        139     785
 
EcoICRI GAG'CTC

Cuts at:      0     571     924 
   Size:        571     353
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     448     889     924 
   Size:        196     252     441      35

  Fragments arranged by size: 

                 441     252     196      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     237     245     289     290     648     758     924 
   Size:        237       8      44       1     358     110     166

  Fragments arranged by size: 

                 358     237     166     110      44       8       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     120     720     924 
   Size:        120     600     204

  Fragments arranged by size: 

                 600     204     120
 
FspI TGC'GCA

Cuts at:      0     475     898     924 
   Size:        475     423      26

  Fragments arranged by size: 

                 475     423      26
 
HaeII r_GCGC'y

Cuts at:      0     423     592     924 
   Size:        423     169     332

  Fragments arranged by size: 

                 423     332     169
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     343     375     742     774     806     924 
   Size:        343      32     367      32      32     118

  Fragments arranged by size: 

                 367     343     118      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0     132     924 
   Size:        132     792
 
MscI TGG'CCA

Cuts at:      0     341     924 
   Size:        341     583
 
MslI CAynn'nnrTG

Cuts at:      0     347     634     829     924 
   Size:        347     287     195      95

  Fragments arranged by size: 

                 347     287     195      95
 
MspA1I CmG'CkG

Cuts at:      0     257     661     924 
   Size:        257     404     263

  Fragments arranged by size: 

                 404     263     257
 
NcoI C'CATG_G

Cuts at:      0     456     924 
   Size:        456     468
 
NspI r_CATG'y

Cuts at:      0     903     924 
   Size:        903      21
 
PfoI T'CCnGG_A

Cuts at:      0     233     760     878     924 
   Size:        233     527     118      46

  Fragments arranged by size: 

                 527     233     118      46
 
PmlI CAC'GTG

Cuts at:      0     710     924 
   Size:        710     214
 
PpuMI rG'GwC_Cy

Cuts at:      0     237     648     758     924 
   Size:        237     411     110     166

  Fragments arranged by size: 

                 411     237     166     110
 
PspOMI G'GGCC_C

Cuts at:      0     245     289     924 
   Size:        245      44     635

  Fragments arranged by size: 

                 635     245      44
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     481     513     924 
   Size:        481      32     411

  Fragments arranged by size: 

                 481     411      32
 
PstI C_TGCA'G

Cuts at:      0     197     924 
   Size:        197     727
 
PvuII CAG'CTG

Cuts at:      0     257     924 
   Size:        257     667
 
SacI G_AGCT'C

Cuts at:      0     573     924 
   Size:        573     351
 
SapI GCTCTTCn'nnn_

Cuts at:      0     316     442     721     924 
   Size:        316     126     279     203

  Fragments arranged by size: 

                 316     279     203     126
 
SfcI C'TryA_G

Cuts at:      0     193     924 
   Size:        193     731
 
SphI G_CATG'C

Cuts at:      0     903     924 
   Size:        903      21
 
StyI C'CwwG_G

Cuts at:      0     161     456     845     924 
   Size:        161     295     389      79

  Fragments arranged by size: 

                 389     295     161      79
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     249     841     924 
   Size:        249     592      83

  Fragments arranged by size: 

                 592     249      83
 
TatI w'GTAC_w

Cuts at:      0     227     924 
   Size:        227     697
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      34      66     215     247     436     468     924 
   Size:         34      32     149      32     189      32     456

  Fragments arranged by size: 

                 456     189     149      34      32      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     103     703     874     924 
   Size:        103     600     171      50

  Fragments arranged by size: 

                 600     171     103      50
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AhdI    AlwNI     ApaI    ApaLI     BanI
    BanII     BbsI     BglI     BlpI Bme1580I     BmrI     BpmI    BsaAI
    BsaBI    BsaXI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI
    BspMI    BsrDI   BstAPI    BstXI    BstYI     BtgI     BtsI     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI     FspI    HaeII
    Hin4I     KpnI     MscI     MslI   MspA1I     NcoI     NspI     PfoI
     PmlI    PpuMI   PspOMI     PsrI     PstI    PvuII     SacI     SapI
     SfcI     SphI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoNI    EcoRI
    EcoRV     FseI    FspAI   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI    PspXI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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