Restriction map for pF1KB3392
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoT22I     FauI        
FseI        FspI        HaeII       Hin1I       HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MslI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          463  GTCTAC               GT^[AC][GT]AC
AciI          103  GCGG                 CCGC(-3/-1)
AcsI           16  GAATTC               [GA]^AATT[CT]
AfaI          726  GTAC                 GT^AC
AflIII        656  ACGTGT               A^C[GA][CT]GT
AlwI           97  GGATC                GGATC(4/5)
ApoI           16  GAATTC               [GA]^AATT[CT]
Asp700I        58  GAATATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          491  TGGCCA               TGG^CCA
BanII         108  GAGCCC               G[GA]GC[CT]^C
BbsI          586  GAAGAC               GAAGAC(2/6)
BclI          887  TGATCA               T^GATCA
BcnI           94  CCCGG                CC^[GC]GG
BglII         486  AGATCT               A^GATCT
BmyI          108  GAGCCC               G[AGT]GC[ACT]^C
BpuAI         586  GAAGAC               GAAGAC(2/6)
BseRI         313  GAGGAG               GAGGAG(10/8)
BsmAI         449  GAGAC                GTCTC(1/5)
BsmI          937  GCATTC               GAATGC(1/-1)
Bsp1286I      108  GAGCCC               G[AGT]GC[ACT]^C
BsrDI           4  GCAATG               GCAATG(2/0)
BstYI         486  AGATCT               [GA]^GATC[CT]
Cac8I          27  GCAGGC               GC[ACGT]^[ACGT]GC
EaeI          491  TGGCCA               [CT]^GGCC[GA]
EcoRI          16  GAATTC               G^AATTC
EcoRV         928  GATATC               GAT^ATC
EcoT14I       387  CCTTGG               C^C[AT][AT]GG
FbaI          887  TGATCA               T^GATCA
HapII          95  CCGG                 C^CGG
HgaI          228  GCGTC                GACGC(5/10)
HincII        230  GTCAAC               GT[CT]^[GA]AC
HindII        230  GTCAAC               GT[CT]^[GA]AC
HindIII       841  AAGCTT               A^AGCTT
HinfI         598  GATTC                G^A[ACGT]TC
HpaII          95  CCGG                 C^CGG
MflI          486  AGATCT               [GA]^GATC[CT]
MluNI         491  TGGCCA               TGG^CCA
MscI          491  TGGCCA               TGG^CCA
MspA1I        101  CAGCGG               C[AC]G^C[GT]G
MspI           95  CCGG                 C^CGG
NciI           94  CCCGG                CC^[GC]GG
NlaIV          91  GGTCCC               GG[ACGT]^[ACGT]CC
RsaI          726  GTAC                 GT^AC
SapI           49  GAAGAGC              GCTCTTC(1/4)
SfcI          465  CTACAG               C^T[GA][CT]AG
StuI           29  AGGCCT               AGG^CCT
StyI          387  CCTTGG               C^C[AT][AT]GG
TfiI          598  GATTC                G^A[AT]TC
TspRI         284  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         486  AGATCT               [GA]^GATC[CT]
XmnI           58  GAATATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AvaII          91  GGTCC                G^G[AT]CC
              756  GGACC               
BsmFI         643  GGGAC                GGGAC(10/14)
               92  GTCCC               
BstEII        304  GGTCACC              G^GT[ACGT]ACC
              556  GGTGACC             
BstPI         304  GGTCACC              G^GT[ACGT]ACC
              556  GGTGACC             
CfoI          346  GCGC                 GCG^C
              809  GCGC                
DraII         383  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              610  AGGGCCT             
EarI           49  GAAGAG               CTCTTC(1/4)
              480  GAAGAG              
EcoNI          21  CCTCAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              309  CCTTGAGGAGG         
EcoO109I      383  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              610  AGGGCCT             
EcoO65I       304  GGTCACC              G^GT[ACGT]ACC
              556  GGTGACC             
HhaI          346  GCGC                 GCG^C
              809  GCGC                
HinP1I        346  GCGC                 G^CGC
              809  GCGC                
Ksp632I        49  GAAGAG               CTCTTC(1/4)
              480  GAAGAG              
MaeII         650  ACGT                 A^CGT
              656  ACGT                
MseI          170  TTAA                 T^TAA
              455  TTAA                
MwoI          809  GCGCCACAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              826  GCTTTCTTTGC         
SmlI           22  CTCAAG               C^T[CT][GA]AG
              310  CTTGAG              
Tru9I         170  TTAA                 T^TAA
              455  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AluI        BbvI        BpmI        BsaJI       BsiYI       
BslI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MvaI        NdeII       NlaIII      
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
Tsp45I      Tsp509I     
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