Restriction map for pF1KB3392
 (Linear) (Six-base) MAPSORT of: KIBB3392.seq  Check: 9398  from: 1  to: 1038



KIBB3392.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:      0     464    1038 
   Size:        464     574
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     499    1038 
   Size:        307     192     539

  Fragments arranged by size: 

                 539     307     192
 
AflIII A'CryG_T

Cuts at:      0     656    1038 
   Size:        656     382
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     371    1038 
   Size:        371     667
 
ApoI r'AATT_y

Cuts at:      0      16    1038 
   Size:         16    1022
 
BanII G_rGCy'C

Cuts at:      0     112    1038 
   Size:        112     926
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     593    1038 
   Size:        593     445
 
BclI T'GATC_A

Cuts at:      0     887    1038 
   Size:        887     151
 
BglII A'GATC_T

Cuts at:      0     486    1038 
   Size:        486     552
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     326     539     781    1038 
   Size:        238      88     213     242     257

  Fragments arranged by size: 

                 257     242     238     213      88
 
Bpu10I CC'TnA_GC

Cuts at:      0     551    1038 
   Size:        551     487
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     331    1038 
   Size:          7     324     707

  Fragments arranged by size: 

                 707     324       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     328    1038 
   Size:        328     710
 
BsmI GAATG_Cn'

Cuts at:      0     937    1038 
   Size:        937     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     112    1038 
   Size:        112     926
 
BsrDI GCAATG_nn'

Cuts at:      0      11    1038 
   Size:         11    1027
 
BstEII G'GTnAC_C

Cuts at:      0     304     556    1038 
   Size:        304     252     482

  Fragments arranged by size: 

                 482     304     252
 
BstYI r'GATC_y

Cuts at:      0     486    1038 
   Size:        486     552
 
EaeI y'GGCC_r

Cuts at:      0     491    1038 
   Size:        491     547
 
EarI CTCTTCn'nnn_

Cuts at:      0      44     475    1038 
   Size:         44     431     563

  Fragments arranged by size: 

                 563     431      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     307     326     499     539     781    1038 
   Size:        238      69      19     173      40     242     257

  Fragments arranged by size: 

                 257     242     238     173      69      40      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      25     313    1038 
   Size:         25     288     725

  Fragments arranged by size: 

                 725     288      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0     384     611    1038 
   Size:        384     227     427

  Fragments arranged by size: 

                 427     384     227
 
EcoRI G'AATT_C

Cuts at:      0      16    1038 
   Size:         16    1022
 
EcoRV GAT'ATC

Cuts at:      0     930    1038 
   Size:        930     108
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      99     131     490     522    1038 
   Size:         99      32     359      32     516

  Fragments arranged by size: 

                 516     359      99      32      32
 
HincII GTy'rAC

Cuts at:      0     232    1038 
   Size:        232     806
 
HindIII A'AGCT_T

Cuts at:      0     841    1038 
   Size:        841     197
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     846    1038 
   Size:        846     192
 
MscI TGG'CCA

Cuts at:      0     493    1038 
   Size:        493     545
 
MspA1I CmG'CkG

Cuts at:      0     103    1038 
   Size:        103     935
 
PfoI T'CCnGG_A

Cuts at:      0      93     254     980    1038 
   Size:         93     161     726      58

  Fragments arranged by size: 

                 726     161      93      58
 
SapI GCTCTTCn'nnn_

Cuts at:      0      44    1038 
   Size:         44     994
 
SfcI C'TryA_G

Cuts at:      0     465    1038 
   Size:        465     573
 
SmlI C'TyrA_G

Cuts at:      0      22     310    1038 
   Size:         22     288     728

  Fragments arranged by size: 

                 728     288      22
 
StuI AGG'CCT

Cuts at:      0      31    1038 
   Size:         31    1007
 
StyI C'CwwG_G

Cuts at:      0     387    1038 
   Size:        387     651
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     575    1038 
   Size:        575     463
 
TatI w'GTAC_w

Cuts at:      0     725    1038 
   Size:        725     313
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      89     121    1038 
   Size:         89      32     917

  Fragments arranged by size: 

                 917      89      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      62    1038 
   Size:         62     976
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AlfI     ApoI    BanII     BbsI     BclI
    BglII     BpmI   Bpu10I    BpuEI    BseRI     BsmI Bsp1286I    BsrDI
   BstEII    BstYI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV    Hin4I   HincII  HindIII     MmeI     MscI   MspA1I     PfoI
     SapI     SfcI     SmlI     StuI     StyI    TaqII     TatI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI     FalI     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI
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