Restriction map for pF1KE0985
 (Linear) (Six-base) MAPSORT of: KIEE0985.seq  Check: 2944  from: 1  to: 1788



KIEE0985.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     438    1788 
   Size:        438    1350
 
Acc65I G'GTAC_C

Cuts at:      0     190    1788 
   Size:        190    1598
 
AclI AA'CG_TT

Cuts at:      0    1478    1788 
   Size:       1478     310
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169    1336    1788 
   Size:        169    1167     452

  Fragments arranged by size: 

                1167     452     169
 
AfeI AGC'GCT

Cuts at:      0    1731    1788 
   Size:       1731      57
 
AflII C'TTAA_G

Cuts at:      0    1660    1678    1788 
   Size:       1660      18     110

  Fragments arranged by size: 

                1660     110      18
 
AflIII A'CryG_T

Cuts at:      0    1391    1788 
   Size:       1391     397
 
AleI CACnn'nnGTG

Cuts at:      0    1150    1788 
   Size:       1150     638
 
ApaI G_GGCC'C

Cuts at:      0     257     278    1581    1788 
   Size:        257      21    1303     207

  Fragments arranged by size: 

                1303     257     207      21
 
ApoI r'AATT_y

Cuts at:      0      32     844    1788 
   Size:         32     812     944

  Fragments arranged by size: 

                 944     812      32
 
AvaI C'yCGr_G

Cuts at:      0     672    1761    1788 
   Size:        672    1089      27

  Fragments arranged by size: 

                1089     672      27
 
BamHI G'GATC_C

Cuts at:      0     135     363     558    1178    1788 
   Size:        135     228     195     620     610

  Fragments arranged by size: 

                 620     610     228     195     135
 
BanI G'GyrC_C

Cuts at:      0      57     190     534    1788 
   Size:         57     133     344    1254

  Fragments arranged by size: 

                1254     344     133      57
 
BanII G_rGCy'C

Cuts at:      0     257     278     427     521    1100    1581    1788 
   Size:        257      21     149      94     579     481     207

  Fragments arranged by size: 

                 579     481     257     207     149      94      21
 
BbvCI CC'TCA_GC

Cuts at:      0     820    1788 
   Size:        820     968
 
BclI T'GATC_A

Cuts at:      0    1409    1788 
   Size:       1409     379
 
BglI GCCn_nnn'nGGC

Cuts at:      0     644    1788 
   Size:        644    1144
 
BglII A'GATC_T

Cuts at:      0    1166    1670    1788 
   Size:       1166     504     118

  Fragments arranged by size: 

                1166     504     118
 
BlpI GC'TnA_GC

Cuts at:      0     338    1426    1788 
   Size:        338    1088     362

  Fragments arranged by size: 

                1088     362     338
 
Bme1580I G_kGCm'C

Cuts at:      0     257     278     539    1581    1770    1788 
   Size:        257      21     261    1042     189      18

  Fragments arranged by size: 

                1042     261     257     189      21      18
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1590    1788 
   Size:       1590     198
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     453    1006    1788 
   Size:        453     553     782

  Fragments arranged by size: 

                 782     553     453
 
Bpu10I CC'TnA_GC

Cuts at:      0     820    1788 
   Size:        820     968
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     910    1204    1788 
   Size:        910     294     584

  Fragments arranged by size: 

                 910     584     294
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     307    1459    1504    1788 
   Size:        307    1152      45     284

  Fragments arranged by size: 

                1152     307     284      45
 
BsaHI Gr'CG_yC

Cuts at:      0    1656    1788 
   Size:       1656     132
 
BsaWI w'CCGG_w

Cuts at:      0     132    1788 
   Size:        132    1656
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1360    1390    1534    1564    1788 
   Size:       1360      30     144      30     224

  Fragments arranged by size: 

                1360     224     144      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     763    1788 
   Size:        763    1025
 
BseYI C'CCAG_C

Cuts at:      0      27     271     293     448    1788 
   Size:         27     244      22     155    1340

  Fragments arranged by size: 

                1340     244     155      27      22
 
BsiHKAI G_wGCw'C

Cuts at:      0     521    1100    1788 
   Size:        521     579     688

  Fragments arranged by size: 

                 688     579     521
 
Bsp1286I G_dGCh'C

Cuts at:      0     257     278     427     521     539    1100    1581    1770 
   Size:        257      21     149      94      18     561     481     189

Cuts at:   1770    1788 
   Size:         18

  Fragments arranged by size: 

                 561     481     257     189     149      94      21      18
                  18
 
BspEI T'CCGG_A

Cuts at:      0     132    1788 
   Size:        132    1656
 
BspHI T'CATG_A

Cuts at:      0     998    1691    1788 
   Size:        998     693      97

  Fragments arranged by size: 

                 998     693      97
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     438     910    1788 
   Size:        438     472     878

  Fragments arranged by size: 

                 878     472     438
 
BsrBI CCG'CTC

Cuts at:      0    1430    1788 
   Size:       1430     358
 
BsrDI GCAATG_nn'

Cuts at:      0    1718    1788 
   Size:       1718      70
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     367    1788 
   Size:        367    1421
 
BstYI r'GATC_y

Cuts at:      0     135     363     558    1166    1178    1670    1788 
   Size:        135     228     195     608      12     492     118

  Fragments arranged by size: 

                 608     492     228     195     135     118      12
 
Bsu36I CC'TnA_GG

Cuts at:      0     351    1352    1788 
   Size:        351    1001     436

  Fragments arranged by size: 

                1001     436     351
 
BtgI C'CryG_G

Cuts at:      0     257     554    1148    1788 
   Size:        257     297     594     640

  Fragments arranged by size: 

                 640     594     297     257
 
BtsI GCAGTG_nn'

Cuts at:      0     273     846    1788 
   Size:        273     573     942

  Fragments arranged by size: 

                 942     573     273
 
EaeI y'GGCC_r

Cuts at:      0     488     645    1788 
   Size:        488     157    1143

  Fragments arranged by size: 

                1143     488     157
 
EarI CTCTTCn'nnn_

Cuts at:      0    1105    1189    1502    1690    1788 
   Size:       1105      84     313     188      98

  Fragments arranged by size: 

                1105     313     188      98      84
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1106    1645    1788 
   Size:       1106     539     143

  Fragments arranged by size: 

                1106     539     143
 
EcoICRI GAG'CTC

Cuts at:      0     519    1098    1788 
   Size:        519     579     690

  Fragments arranged by size: 

                 690     579     519
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     453    1006    1336    1788 
   Size:        169     284     553     330     452

  Fragments arranged by size: 

                 553     452     330     284     169
 
EcoO109I rG'GnC_Cy

Cuts at:      0      67     223     253     275     726    1331    1577    1788 
   Size:         67     156      30      22     451     605     246     211

  Fragments arranged by size: 

                 605     451     246     211     156      67      30      22
            
 
EcoRI G'AATT_C

Cuts at:      0      32     844    1788 
   Size:         32     812     944

  Fragments arranged by size: 

                 944     812      32
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1341    1731    1788 
   Size:       1341     390      57

  Fragments arranged by size: 

                1341     390      57
 
HaeII r_GCGC'y

Cuts at:      0     871    1704    1733    1788 
   Size:        871     833      29      55

  Fragments arranged by size: 

                 871     833      55      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1330    1360    1362    1392    1510    1534    1542    1566 
   Size:       1330      30       2      30     118      24       8      24

Cuts at:   1566    1788 
   Size:        222

  Fragments arranged by size: 

                1330     222     118      30      30      24      24       8
                   2
 
HincII GTy'rAC

Cuts at:      0    1245    1788 
   Size:       1245     543
 
KpnI G_GTAC'C

Cuts at:      0     194    1788 
   Size:        194    1594
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1577    1788 
   Size:       1577     211
 
MscI TGG'CCA

Cuts at:      0     490     647    1788 
   Size:        490     157    1141

  Fragments arranged by size: 

                1141     490     157
 
MslI CAynn'nnrTG

Cuts at:      0    1150    1417    1788 
   Size:       1150     267     371

  Fragments arranged by size: 

                1150     371     267
 
MspA1I CmG'CkG

Cuts at:      0     259     452    1008    1032    1788 
   Size:        259     193     556      24     756

  Fragments arranged by size: 

                 756     556     259     193      24
 
NcoI C'CATG_G

Cuts at:      0    1148    1788 
   Size:       1148     640
 
NspI r_CATG'y

Cuts at:      0    1395    1422    1788 
   Size:       1395      27     366

  Fragments arranged by size: 

                1395     366      27
 
PasI CC'CwG_GG

Cuts at:      0     377    1788 
   Size:        377    1411
 
PciI A'CATG_T

Cuts at:      0    1391    1788 
   Size:       1391     397
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     768    1788 
   Size:        768    1020
 
PfoI T'CCnGG_A

Cuts at:      0     366    1788 
   Size:        366    1422
 
PpuMI rG'GwC_Cy

Cuts at:      0      67    1331    1788 
   Size:         67    1264     457

  Fragments arranged by size: 

                1264     457      67
 
PshAI GACnn'nnGTC

Cuts at:      0     319    1788 
   Size:        319    1469
 
PspOMI G'GGCC_C

Cuts at:      0     253     274    1577    1788 
   Size:        253      21    1303     211

  Fragments arranged by size: 

                1303     253     211      21
 
PspXI vC'TCGA_Gb

Cuts at:      0     672    1788 
   Size:        672    1116
 
PstI C_TGCA'G

Cuts at:      0      96    1788 
   Size:         96    1692
 
PvuII CAG'CTG

Cuts at:      0     452    1008    1032    1788 
   Size:        452     556      24     756

  Fragments arranged by size: 

                 756     556     452      24
 
SacI G_AGCT'C

Cuts at:      0     521    1100    1788 
   Size:        521     579     688

  Fragments arranged by size: 

                 688     579     521
 
SacII CC_GC'GG

Cuts at:      0     260    1788 
   Size:        260    1528
 
SanDI GG'GwC_CC

Cuts at:      0    1331    1788 
   Size:       1331     457
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1105    1788 
   Size:       1105     683
 
SfcI C'TryA_G

Cuts at:      0      92    1788 
   Size:         92    1696
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     644    1788 
   Size:        644    1144
 
SmlI C'TyrA_G

Cuts at:      0     672     925    1219    1660    1678    1788 
   Size:        672     253     294     441      18     110

  Fragments arranged by size: 

                 672     441     294     253     110      18
 
StuI AGG'CCT

Cuts at:      0     923    1788 
   Size:        923     865
 
StyI C'CwwG_G

Cuts at:      0     219     332     572     860     938    1148    1788 
   Size:        219     113     240     288      78     210     640

  Fragments arranged by size: 

                 640     288     240     219     210     113      78
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     156    1150    1788 
   Size:        156     994     638

  Fragments arranged by size: 

                 994     638     156
 
TatI w'GTAC_w

Cuts at:      0    1079    1788 
   Size:       1079     709
 
Tth111I GACn'n_nGTC

Cuts at:      0    1546    1788 
   Size:       1546     242
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1257    1788 
   Size:       1257     531
 
XhoI C'TCGA_G

Cuts at:      0     672    1788 
   Size:        672    1116
 
XmnI GAAnn'nnTTC

Cuts at:      0    1714    1788 
   Size:       1714      74
 

 Enzymes that do cut:

     AarI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AleI
     ApaI     ApoI     AvaI    BamHI     BanI    BanII    BbvCI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII     KpnI     MmeI     MscI
     MslI   MspA1I     NcoI     NspI     PasI     PciI    PflMI     PfoI
    PpuMI    PshAI   PspOMI    PspXI     PstI    PvuII     SacI    SacII
    SanDI     SapI     SfcI     SfiI     SmlI     StuI     StyI    TaqII
     TatI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI     BcgI
    BciVI    BfrBI    BmgBI     BmtI     BplI    BsaAI    BsaBI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI    EcoNI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI
     PpiI     PsiI     PsrI     PvuI    RsrII     SalI     SbfI     ScaI
    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI     XbaI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com