################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccIII AcsI AflII AflIII AhdI AlwI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BpuAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstPI Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HinfI HpaI KpnI MamI MluI MroI MseI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PleI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AclI 1093 AACGTT AA^CGTT AgeI 9 ACCGGT A^CCGGT ApaI 518 GGGCCC GGGCC^C AvaI 481 CCCGAG C^[CT]CG[GA]G AviII 1442 TGCGCA TGC^GCA BalI 1649 TGGCCA TGG^CCA BfaI 508 CTAG C^TAG BglII 287 AGATCT A^GATCT BlpI 1042 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1042 GCTGAGC GC^T[ACGT]AGC BsaWI 9 ACCGGT [AT]^CCGG[AT] BsiEI 7 CGACCG CG[GA][CT]^CG BsoBI 481 CCCGAG C^[CT]CG[GA]G BssHII 1461 GCGCGC G^CGCGC Bst1107I 486 GTATAC GTA^TAC BstXI 1427 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 287 AGATCT [GA]^GATC[CT] BstZ17I 486 GTATAC GTA^TAC CelII 1042 GCTGAGC GC^T[ACGT]AGC DpnII 288 GATC ^GATC FspI 1442 TGCGCA TGC^GCA HgaI 1225 GACGC GACGC(5/10) HincII 967 GTCAAC GT[CT]^[GA]AC HindII 967 GTCAAC GT[CT]^[GA]AC MaeI 508 CTAG C^TAG MaeIII 377 GTCAC ^GT[ACGT]AC MboI 288 GATC ^GATC MfeI 614 CAATTG C^AATTG MflI 287 AGATCT [GA]^GATC[CT] MluNI 1649 TGGCCA TGG^CCA MscI 1649 TGGCCA TGG^CCA MunI 614 CAATTG C^AATTG NaeI 955 GCCGGC GCC^GGC NdeII 288 GATC ^GATC NgoMIV 955 GCCGGC G^CCGGC PinAI 9 ACCGGT A^CCGGT Psp1406I 1093 AACGTT AA^CGTT PstI 283 CTGCAG CTGCA^G SapI 588 GCTCTTC GCTCTTC(1/4) Sau3AI 288 GATC ^GATC SfcI 283 CTGCAG C^T[GA][CT]AG StuI 1034 AGGCCT AGG^CCT Tsp45I 377 GTCAC ^GT[GC]AC Tsp509I 615 AATT ^AATT XcmI 155 CCACCGCGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 287 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AccI 486 GTATAC GT^[AC][GT]AC 1567 GTCTAC AfaI 690 GTAC GT^AC 1728 GTAC Aor51HI 857 AGCGCT AGC^GCT 1382 AGCGCT BsaAI 433 TACGTG [CT]AC^GT[GA] 1554 CACGTA EaeI 64 TGGCCG [CT]^GGCC[GA] 1649 TGGCCA Eco47III 857 AGCGCT AGC^GCT 1382 AGCGCT EcoNI 630 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 813 CCTGGCCCAGG HphI 363 GGTGA GGTGA(8/7) 378 TCACC KspI 158 CCGCGG CCGC^GG 1420 CCGCGG RsaI 690 GTAC GT^AC 1728 GTAC SacII 158 CCGCGG CCGC^GG 1420 CCGCGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcyI AluI Alw44I AlwNI ApaLI AspHI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI Ksp632I MaeII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV PflMI PpuMI PvuII SacI Sau96I ScrFI SfaNI SfoI SmlI StyI TaqI TseI TspRI TthHB8I Van91I