Restriction map for pF1KE0945
 (Circular) (Six-base) MAPSORT of: pF1KE0945.seq  Check: 1123  from: 1  to: 4163



pF1KE0945  4163 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AccI GT'mk_AC

Cuts at:   1201    1201 
   Size:       4163
 
Acc65I G'GTAC_C

Cuts at:   1179    1179 
   Size:       4163
 
AclI AA'CG_TT

Cuts at:   4003    4003 
   Size:       4163
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    270     379     753    1988    2420    3167    3435     270 
   Size:        109     374    1235     432     747     268     998

  Fragments arranged by size: 

                1235     998     747     432     374     268     109
 
AflIII A'CryG_T

Cuts at:    504     626    1515    2619    3596     504 
   Size:        122     889    1104     977    1071

  Fragments arranged by size: 

                1104    1071     977     889     122
 
AgeI A'CCGG_T

Cuts at:   2551    2551 
   Size:       4163
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    437     437 
   Size:       4163
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    802     834     802 
   Size:         32    4131
 
AlwNI CAG_nnn'CTG

Cuts at:    483     753    1468    3035     483 
   Size:        270     715    1567    1611

  Fragments arranged by size: 

                1611    1567     715     270
 
ApaLI G'TGCA_C

Cuts at:   2933    2933 
   Size:       4163
 
ApoI r'AATT_y

Cuts at:   1167    3516    3772    1167 
   Size:       2349     256    1558

  Fragments arranged by size: 

                2349    1558     256
 
AseI AT'TA_AT

Cuts at:     20     674      20 
   Size:        654    3509
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4163
 
AvaI C'yCGr_G

Cuts at:    149    1183     149 
   Size:       1034    3129
 
BamHI G'GATC_C

Cuts at:   1188    1188 
   Size:       4163
 
BanI G'GyrC_C

Cuts at:    156    1179    1593    1841    1876     156 
   Size:       1023     414     248      35    2443

  Fragments arranged by size: 

                2443    1023     414     248      35
 
BanII G_rGCy'C

Cuts at:   1177    2207    1177 
   Size:       1030    3133
 
BbeI G_GCGC'C

Cuts at:   1597    1845    1597 
   Size:        248    3915
 
BbsI GAAGACnn'nnnn_

Cuts at:    742     742 
   Size:       4163
 
BciVI GTATCCnnnnn_n'

Cuts at:   1107    2061    2822    4163    1107 
   Size:        954     761    1341    1107

  Fragments arranged by size: 

                1341    1107     954     761
 
BclI T'GATC_A

Cuts at:    182    1683     182 
   Size:       1501    2662
 
BglI GCCn_nnn'nGGC

Cuts at:    698     698 
   Size:       4163
 
BglII A'GATC_T

Cuts at:   1678    3715    1678 
   Size:       2037    2126
 
BlpI GC'TnA_GC

Cuts at:   1275    1275 
   Size:       4163
 
Bme1580I G_kGCm'C

Cuts at:    703     992    1788    1881    2937     703 
   Size:        289     796      93    1056    1929

  Fragments arranged by size: 

                1929    1056     796     289      93
 
BmgBI CAC'GTC

Cuts at:    903     903 
   Size:       4163
 
BmrI ACTGGGnnnn_n'

Cuts at:    741    1375    1456    1791    3935     741 
   Size:        634      81     335    2144     969

  Fragments arranged by size: 

                2144     969     634     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1073    2530    1073 
   Size:       1457    2706
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    466     664     857     957    1111     466 
   Size:        198     193     100     154    3518

  Fragments arranged by size: 

                3518     198     193     154     100
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    597    1339    2218    2710    3008     597 
   Size:        742     879     492     298    1752

  Fragments arranged by size: 

                1752     879     742     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1087    3867    1087 
   Size:       2780    1383
 
BsaAI yAC'GTr

Cuts at:    629    1516     629 
   Size:        887    3276
 
BsaBI GATnn'nnATC

Cuts at:      6    1677    3714       6 
   Size:       1671    2037     455

  Fragments arranged by size: 

                2037    1671     455
 
BsaHI Gr'CG_yC

Cuts at:    794    1594    1842    2544    4040     794 
   Size:        800     248     702    1496     917

  Fragments arranged by size: 

                1496     917     800     702     248
 
BsaWI w'CCGG_w

Cuts at:   1352    1873    2551    2825    2972    3383    1352 
   Size:        521     678     274     147     411    2132

  Fragments arranged by size: 

                2132     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    802     832     887     917     802 
   Size:         30      55      30    4048

  Fragments arranged by size: 

                4048      55      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    230     346     230 
   Size:        116    4047
 
BseYI C'CCAG_C

Cuts at:   1588    2346    2923    3419    1588 
   Size:        758     577     496    2332

  Fragments arranged by size: 

                2332     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    667     667 
   Size:       4163
 
BsiEI CG_ry'CG

Cuts at:    104    1705    1751    2959     104 
   Size:       1601      46    1208    1308

  Fragments arranged by size: 

                1601    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    416    1177    1955    2145    2937     416 
   Size:        761     778     190     792    1642

  Fragments arranged by size: 

                1642     792     778     761     190
 
Bsp1286I G_dGCh'C

Cuts at:    416     703     992    1177    1788    1881    1955    2145    2207 
   Size:        287     289     185     611      93      74     190      62

Cuts at:   2207    2937     416 
   Size:        730    1642

  Fragments arranged by size: 

                1642     730     611     289     287     190     185      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   3383    3383 
   Size:       4163
 
BspHI T'CATG_A

Cuts at:   3339    3339 
   Size:       4163
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    689     737    1213    1427    1729    2110     689 
   Size:         48     476     214     302     381    2742

  Fragments arranged by size: 

                2742     476     381     302     214      48
 
BsrBI CCG'CTC

Cuts at:     50    2455    2509    3993    4158      50 
   Size:       2405      54    1484     165      55

  Fragments arranged by size: 

                2405    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2075    2075 
   Size:       4163
 
BsrFI r'CCGG_y

Cuts at:   2161    2342    2551    2161 
   Size:        181     209    3773

  Fragments arranged by size: 

                3773     209     181
 
BssSI C'ACGA_G

Cuts at:   2434    2792    2434 
   Size:        358    3805
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1540    1540 
   Size:       4163
 
BstBI TT'CG_AA

Cuts at:   2524    2524 
   Size:       4163
 
BstYI r'GATC_y

Cuts at:      1     959    1188    1655    1678    2013    2259    3271    3357 
   Size:        958     229     467      23     335     246    1012      86

Cuts at:   3357    3369    3715       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     958     467     449     346     335     246     229
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     468     653     107 
   Size:        361     185    3617

  Fragments arranged by size: 

                3617     361     185
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    219    2113    2294    2457     219 
   Size:       1894     181     163    1925

  Fragments arranged by size: 

                1925    1894     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1073    1406    1073 
   Size:        333    3830
 
DraI TTT'AAA

Cuts at:   1162    1162 
   Size:       4163
 
DrdI GACnn_nn'nnGTC

Cuts at:   1869    2727    1869 
   Size:        858    3305
 
EaeI y'GGCC_r

Cuts at:    650     840    1748    1922    2313    2340    3685     650 
   Size:        190     908     174     391      27    1345    1128

  Fragments arranged by size: 

                1345    1128     908     391     190     174      27
 
EagI C'GGCC_G

Cuts at:   1748    1748 
   Size:       4163
 
EarI CTCTTCn'nnn_

Cuts at:   2186    2396    2186 
   Size:        210    3953
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2371    2681    2827    3973    2371 
   Size:        310     146    1146    2561

  Fragments arranged by size: 

                2561    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1175    1175 
   Size:       4163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    270     379     466     664     753     857     957    1111    1988 
   Size:        109      87     198      89     104     100     154     877

Cuts at:   1988    2420    3167    3435     270 
   Size:        432     747     268     998

  Fragments arranged by size: 

                 998     877     747     432     268     198     154     109
                 104     100      89      87
 
EcoO109I rG'GnC_Cy

Cuts at:   1302    1302 
   Size:       4163
 
EcoRI G'AATT_C

Cuts at:   1167    1167 
   Size:       4163
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    408    2182     408 
   Size:       1774    2389
 
FspI TGC'GCA

Cuts at:   1944    1944 
   Size:       4163
 
HaeII r_GCGC'y

Cuts at:   1597    1845    2867    3846    1597 
   Size:        248    1022     979    1914

  Fragments arranged by size: 

                1914    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    186     218    2005    2035    2037    2067     186 
   Size:         32    1787      30       2      30    2282

  Fragments arranged by size: 

                2282    1787      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1202    1202 
   Size:       4163
 
KasI G'GCGC_C

Cuts at:   1593    1841    1593 
   Size:        248    3915
 
KpnI G_GTAC'C

Cuts at:   1183    1183 
   Size:       4163
 
MluI A'CGCG_T

Cuts at:   3596    3596 
   Size:       4163
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    746    2834    3018     746 
   Size:       2088     184    1891

  Fragments arranged by size: 

                2088    1891     184
 
MscI TGG'CCA

Cuts at:    652    1924     652 
   Size:       1272    2891
 
MslI CAynn'nnrTG

Cuts at:   2152    2279    2152 
   Size:        127    4036
 
MspA1I CmG'CkG

Cuts at:    166     357    1274    1588    1948    2961    3206     166 
   Size:        191     917     314     360    1013     245    1123

  Fragments arranged by size: 

                1123    1013     917     360     314     245     191
 
NaeI GCC'GGC

Cuts at:   2344    2344 
   Size:       4163
 
NarI GG'CG_CC

Cuts at:   1594    1842    1594 
   Size:        248    3915
 
NcoI C'CATG_G

Cuts at:    107     468     653     107 
   Size:        361     185    3617

  Fragments arranged by size: 

                3617     361     185
 
NdeI CA'TA_TG

Cuts at:    933     933 
   Size:       4163
 
NgoMIV G'CCGG_C

Cuts at:   2342    2342 
   Size:       4163
 
NspI r_CATG'y

Cuts at:    508    1046    1216    1716    2623     508 
   Size:        538     170     500     907    2048

  Fragments arranged by size: 

                2048     907     538     500     170
 
PciI A'CATG_T

Cuts at:    504    2619     504 
   Size:       2115    2048
 
PflMI CCAn_nnn'nTGG

Cuts at:    169     169 
   Size:       4163
 
PfoI T'CCnGG_A

Cuts at:    820     820 
   Size:       4163
 
PmeI GTTT'AAAC

Cuts at:   1162    1162 
   Size:       4163
 
PmlI CAC'GTG

Cuts at:    629     629 
   Size:       4163
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    802     834    3302    3334     802 
   Size:         32    2468      32    1631

  Fragments arranged by size: 

                2468    1631      32      32
 
PstI C_TGCA'G

Cuts at:    479    1210    1895     479 
   Size:        731     685    2747

  Fragments arranged by size: 

                2747     731     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4163
 
PvuII CAG'CTG

Cuts at:    166     357    1588    1948     166 
   Size:        191    1231     360    2381

  Fragments arranged by size: 

                2381    1231     360     191
 
RsrII CG'GwC_CG

Cuts at:   2358    2358 
   Size:       4163
 
SacI G_AGCT'C

Cuts at:   1177    1177 
   Size:       4163
 
SalI G'TCGA_C

Cuts at:   1200    1200 
   Size:       4163
 
SapI GCTCTTCn'nnn_

Cuts at:   2186    2396    2186 
   Size:        210    3953
 
SbfI CC_TGCA'GG

Cuts at:   1210    1210 
   Size:       4163
 
ScaI AGT'ACT

Cuts at:   3680    3680 
   Size:       4163
 
SfcI C'TryA_G

Cuts at:     33     475    1206    1891    2884    3075      33 
   Size:        442     731     685     993     191    1121

  Fragments arranged by size: 

                1121     993     731     685     442     191
 
SfoI GGC'GCC

Cuts at:   1595    1843    1595 
   Size:        248    3915
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4163
 
SmaI CCC'GGG

Cuts at:   1185    1185 
   Size:       4163
 
SmlI C'TyrA_G

Cuts at:    576    1318    2233    2725    2987     576 
   Size:        742     915     492     262    1752

  Fragments arranged by size: 

                1752     915     742     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4163
 
SphI G_CATG'C

Cuts at:   1046    1216    1716    1046 
   Size:        170     500    3493

  Fragments arranged by size: 

                3493     500     170
 
StuI AGG'CCT

Cuts at:    140     140 
   Size:       4163
 
StyI C'CwwG_G

Cuts at:    107     316     468     495     653    1297    1651     107 
   Size:        209     152      27     158     644     354    2619

  Fragments arranged by size: 

                2619     644     354     209     158     152      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1746    1879    2338    2547    1746 
   Size:        133     459     209    3362

  Fragments arranged by size: 

                3362     459     209     133
 
TatI w'GTAC_w

Cuts at:   3678    3678 
   Size:       4163
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    255     287     887     919    3680    3712     255 
   Size:         32     600      32    2761      32     706

  Fragments arranged by size: 

                2761     706     600      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    661     741    1960     661 
   Size:         80    1219    2864

  Fragments arranged by size: 

                2864    1219      80
 
XbaI T'CTAG_A

Cuts at:   1194    1194 
   Size:       4163
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    881     981    1658     881 
   Size:        100     677    3386

  Fragments arranged by size: 

                3386     677     100
 
XmaI C'CCGG_G

Cuts at:   1183    1183 
   Size:       4163
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI     AloI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI     BmtI   Bpu10I    BsiWI
     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI
     XmnI     ZraI
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