(Circular) (Six-base) MAPSORT of: pF1KE0944.seq Check: 9351 from: 1 to: 4142 pF1KE0944 4142 bp With 182 enzymes: SgfI * April 24, 2009 13:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 544 544 Size: 4142 AatII G_ACGT'C Cuts at: 1103 1103 Size: 4142 AccI GT'mk_AC Cuts at: 124 1180 124 Size: 1056 3086 Acc65I G'GTAC_C Cuts at: 860 1158 860 Size: 298 3844 AclI AA'CG_TT Cuts at: 1136 3982 1136 Size: 2846 1296 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 879 1967 2399 3146 3414 879 Size: 1088 432 747 268 1607 Fragments arranged by size: 1607 1088 747 432 268 AflIII A'CryG_T Cuts at: 815 1494 2598 3575 815 Size: 679 1104 977 1382 Fragments arranged by size: 1382 1104 977 679 AgeI A'CCGG_T Cuts at: 2530 2530 Size: 4142 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 223 255 223 Size: 32 4110 AlwNI CAG_nnn'CTG Cuts at: 1447 3014 1447 Size: 1567 2575 ApaLI G'TGCA_C Cuts at: 2912 2912 Size: 4142 ApoI r'AATT_y Cuts at: 646 1010 1053 1146 3495 3751 646 Size: 364 43 93 2349 256 1037 Fragments arranged by size: 2349 1037 364 256 93 43 AseI AT'TA_AT Cuts at: 20 20 Size: 4142 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4142 AvaI C'yCGr_G Cuts at: 243 253 674 798 1162 243 Size: 10 421 124 364 3223 Fragments arranged by size: 3223 421 364 124 10 BamHI G'GATC_C Cuts at: 1167 1167 Size: 4142 BanI G'GyrC_C Cuts at: 411 860 1092 1158 1572 1820 1855 411 Size: 449 232 66 414 248 35 2698 Fragments arranged by size: 2698 449 414 248 232 66 35 BanII G_rGCy'C Cuts at: 212 250 986 1156 2186 212 Size: 38 736 170 1030 2168 Fragments arranged by size: 2168 1030 736 170 38 BbeI G_GCGC'C Cuts at: 1576 1824 1576 Size: 248 3894 BbsI GAAGACnn'nnnn_ Cuts at: 704 704 Size: 4142 BciVI GTATCCnnnnn_n' Cuts at: 2040 2801 4142 2040 Size: 761 1341 2040 Fragments arranged by size: 2040 1341 761 BclI T'GATC_A Cuts at: 1662 1662 Size: 4142 BfrBI ATG'CAT Cuts at: 519 519 Size: 4142 BglII A'GATC_T Cuts at: 773 1657 3694 773 Size: 884 2037 1221 Fragments arranged by size: 2037 1221 884 BlpI GC'TnA_GC Cuts at: 1254 1254 Size: 4142 Bme1580I G_kGCm'C Cuts at: 1767 1860 2916 1767 Size: 93 1056 2993 Fragments arranged by size: 2993 1056 93 BmgBI CAC'GTC Cuts at: 816 816 Size: 4142 BmrI ACTGGGnnnn_n' Cuts at: 1354 1435 1770 3914 1354 Size: 81 335 2144 1582 Fragments arranged by size: 2144 1582 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2509 2509 Size: 4142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 337 337 Size: 4142 Bpu10I CC'TnA_GC Cuts at: 1070 1070 Size: 4142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 619 1318 2197 2689 2987 619 Size: 699 879 492 298 1774 Fragments arranged by size: 1774 879 699 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 317 469 3846 317 Size: 152 3377 613 Fragments arranged by size: 3377 613 152 BsaAI yAC'GTr Cuts at: 762 1495 762 Size: 733 3409 BsaBI GATnn'nnATC Cuts at: 6 1656 3693 6 Size: 1650 2037 455 Fragments arranged by size: 2037 1650 455 BsaHI Gr'CG_yC Cuts at: 701 1100 1573 1821 2523 4019 701 Size: 399 473 248 702 1496 824 Fragments arranged by size: 1496 824 702 473 399 248 BsaWI w'CCGG_w Cuts at: 1331 1852 2530 2804 2951 3362 1331 Size: 521 678 274 147 411 2111 Fragments arranged by size: 2111 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 223 253 314 344 355 385 223 Size: 30 61 30 11 30 3980 Fragments arranged by size: 3980 61 30 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 376 568 571 376 Size: 192 3 3947 Fragments arranged by size: 3947 192 3 BseYI C'CCAG_C Cuts at: 211 883 1567 2325 2902 3398 211 Size: 672 684 758 577 496 955 Fragments arranged by size: 955 758 684 672 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 334 334 Size: 4142 BsiEI CG_ry'CG Cuts at: 104 1684 1730 2938 104 Size: 1580 46 1208 1308 Fragments arranged by size: 1580 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1156 1934 2124 2916 1156 Size: 778 190 792 2382 Fragments arranged by size: 2382 792 778 190 BsmI GAATG_Cn' Cuts at: 519 968 519 Size: 449 3693 BsmBI CGTCTCn'nnnn_ Cuts at: 1053 1053 Size: 4142 Bsp1286I G_dGCh'C Cuts at: 212 250 986 1156 1767 1860 1934 2124 2186 Size: 38 736 170 611 93 74 190 62 Cuts at: 2186 2916 212 Size: 730 1438 Fragments arranged by size: 1438 736 730 611 190 170 93 74 62 38 BspEI T'CCGG_A Cuts at: 3362 3362 Size: 4142 BspHI T'CATG_A Cuts at: 3318 3318 Size: 4142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 544 1192 1406 1708 2089 544 Size: 648 214 302 381 2597 Fragments arranged by size: 2597 648 381 302 214 BsrBI CCG'CTC Cuts at: 50 993 2434 2488 3972 4137 50 Size: 943 1441 54 1484 165 55 Fragments arranged by size: 1484 1441 943 165 55 54 BsrDI GCAATG_nn' Cuts at: 273 2054 273 Size: 1781 2361 BsrFI r'CCGG_y Cuts at: 2140 2321 2530 2140 Size: 181 209 3752 Fragments arranged by size: 3752 209 181 BsrGI T'GTAC_A Cuts at: 839 839 Size: 4142 BssSI C'ACGA_G Cuts at: 2413 2771 2413 Size: 358 3784 BstAPI GCAn_nnn'nTGC Cuts at: 1066 1519 1066 Size: 453 3689 BstBI TT'CG_AA Cuts at: 179 2503 179 Size: 2324 1818 BstXI CCAn_nnnn'nTGG Cuts at: 852 852 Size: 4142 BstYI r'GATC_y Cuts at: 1 773 902 1167 1634 1657 1992 2238 3250 Size: 772 129 265 467 23 335 246 1012 Cuts at: 3250 3336 3348 3694 1 Size: 86 12 346 449 Fragments arranged by size: 1012 772 467 449 346 335 265 246 129 86 23 12 BtgI C'CryG_G Cuts at: 203 203 Size: 4142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2092 2273 2436 2092 Size: 181 163 3798 Fragments arranged by size: 3798 181 163 BtsI GCAGTG_nn' Cuts at: 599 600 1385 599 Size: 1 785 3356 Fragments arranged by size: 3356 785 1 DraI TTT'AAA Cuts at: 1141 1141 Size: 4142 DrdI GACnn_nn'nnGTC Cuts at: 1848 2706 1848 Size: 858 3284 EaeI y'GGCC_r Cuts at: 912 1727 1901 2292 2319 3664 912 Size: 815 174 391 27 1345 1390 Fragments arranged by size: 1390 1345 815 391 174 27 EagI C'GGCC_G Cuts at: 1727 1727 Size: 4142 EarI CTCTTCn'nnn_ Cuts at: 176 659 898 1106 2165 2375 176 Size: 483 239 208 1059 210 1943 Fragments arranged by size: 1943 1059 483 239 210 208 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2350 2660 2806 3952 2350 Size: 310 146 1146 2540 Fragments arranged by size: 2540 1146 310 146 EcoICRI GAG'CTC Cuts at: 1154 1154 Size: 4142 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 337 879 1967 2399 3146 3414 337 Size: 542 1088 432 747 268 1065 Fragments arranged by size: 1088 1065 747 542 432 268 EcoO109I rG'GnC_Cy Cuts at: 630 1281 630 Size: 651 3491 EcoRI G'AATT_C Cuts at: 1053 1146 1053 Size: 93 4049 EcoRV GAT'ATC Cuts at: 387 387 Size: 4142 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2161 2161 Size: 4142 FspI TGC'GCA Cuts at: 1923 1923 Size: 4142 HaeII r_GCGC'y Cuts at: 1576 1824 2846 3825 1576 Size: 248 1022 979 1893 Fragments arranged by size: 1893 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 175 207 718 750 922 954 1984 2014 2016 Size: 32 511 32 172 32 1030 30 2 Cuts at: 2016 2046 175 Size: 30 2271 Fragments arranged by size: 2271 1030 511 172 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1104 1181 1104 Size: 77 4065 KasI G'GCGC_C Cuts at: 1572 1820 1572 Size: 248 3894 KpnI G_GTAC'C Cuts at: 864 1162 864 Size: 298 3844 MluI A'CGCG_T Cuts at: 3575 3575 Size: 4142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 287 761 2813 2997 287 Size: 474 2052 184 1432 Fragments arranged by size: 2052 1432 474 184 MscI TGG'CCA Cuts at: 914 1903 914 Size: 989 3153 MslI CAynn'nnrTG Cuts at: 605 850 2131 2258 605 Size: 245 1281 127 2489 Fragments arranged by size: 2489 1281 245 127 MspA1I CmG'CkG Cuts at: 205 356 392 1089 1253 1567 1927 2940 3185 Size: 151 36 697 164 314 360 1013 245 Cuts at: 3185 205 Size: 1162 Fragments arranged by size: 1162 1013 697 360 314 245 164 151 36 NaeI GCC'GGC Cuts at: 2323 2323 Size: 4142 NarI GG'CG_CC Cuts at: 1573 1821 1573 Size: 248 3894 NdeI CA'TA_TG Cuts at: 1062 1062 Size: 4142 NgoMIV G'CCGG_C Cuts at: 2321 2321 Size: 4142 NsiI A_TGCA'T Cuts at: 521 521 Size: 4142 NspI r_CATG'y Cuts at: 519 1195 1695 2602 519 Size: 676 500 907 2059 Fragments arranged by size: 2059 907 676 500 PciI A'CATG_T Cuts at: 2598 2598 Size: 4142 PmeI GTTT'AAAC Cuts at: 1141 1141 Size: 4142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 223 255 3281 3313 223 Size: 32 3026 32 1052 Fragments arranged by size: 3026 1052 32 32 PpuMI rG'GwC_Cy Cuts at: 630 630 Size: 4142 PshAI GACnn'nnGTC Cuts at: 676 676 Size: 4142 PstI C_TGCA'G Cuts at: 1189 1874 1189 Size: 685 3457 PvuI CG_AT'CG Cuts at: 104 104 Size: 4142 PvuII CAG'CTG Cuts at: 392 1089 1567 1927 392 Size: 697 478 360 2607 Fragments arranged by size: 2607 697 478 360 RsrII CG'GwC_CG Cuts at: 2337 2337 Size: 4142 SacI G_AGCT'C Cuts at: 1156 1156 Size: 4142 SacII CC_GC'GG Cuts at: 206 206 Size: 4142 SalI G'TCGA_C Cuts at: 1179 1179 Size: 4142 SapI GCTCTTCn'nnn_ Cuts at: 2165 2375 2165 Size: 210 3932 SbfI CC_TGCA'GG Cuts at: 1189 1189 Size: 4142 ScaI AGT'ACT Cuts at: 3659 3659 Size: 4142 SfcI C'TryA_G Cuts at: 33 1034 1185 1870 2863 3054 33 Size: 1001 151 685 993 191 1121 Fragments arranged by size: 1121 1001 993 685 191 151 SfoI GGC'GCC Cuts at: 1574 1822 1574 Size: 248 3894 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4142 SmaI CCC'GGG Cuts at: 1164 1164 Size: 4142 SmlI C'TyrA_G Cuts at: 634 1297 2212 2704 2966 634 Size: 663 915 492 262 1810 Fragments arranged by size: 1810 915 663 492 262 SnaBI TAC'GTA Cuts at: 762 762 Size: 4142 SpeI A'CTAG_T Cuts at: 67 67 Size: 4142 SphI G_CATG'C Cuts at: 1195 1695 1195 Size: 500 3642 StyI C'CwwG_G Cuts at: 944 1024 1276 1630 944 Size: 80 252 354 3456 Fragments arranged by size: 3456 354 252 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 460 1725 1858 2317 2526 460 Size: 1265 133 459 209 2076 Fragments arranged by size: 2076 1265 459 209 133 TatI w'GTAC_w Cuts at: 839 3657 839 Size: 2818 1324 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 355 387 3659 3691 355 Size: 32 3272 32 806 Fragments arranged by size: 3272 806 32 32 Tth111I GACn'n_nGTC Cuts at: 936 1939 936 Size: 1003 3139 XbaI T'CTAG_A Cuts at: 1173 1173 Size: 4142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1637 1637 Size: 4142 XmaI C'CCGG_G Cuts at: 1162 1162 Size: 4142 ZraI GAC'GTC Cuts at: 1101 1101 Size: 4142 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AfeI AflII AhdI AleI AlfI ApaI AscI AvrII BaeI BbvCI BcgI BglI BmtI BsiWI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI NcoI NheI NotI NruI PacI PasI PflMI PfoI PmlI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI