Restriction map for pF1KE0941
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiWI       BsmAI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KspI        
MaeII       MamI        MflI        MluI        MluNI       
MroI        MscI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I         26  GGTACC               G^GTACC
AlwI          750  GATCC                GGATC(4/5)
AlwNI         715  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I        26  GGTACC               G^GTACC
BanI           26  GGTACC               G^G[CT][GA]CC
BbvCI         271  GCTGAGG              CCTCAGC(-5/-2)
BfaI          793  CTAG                 C^TAG
BlpI          492  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      492  GCTGAGC              GC^T[ACGT]AGC
BsiEI         704  CGGCCG               CG[GA][CT]^CG
BsmI          548  GCATTC               GAATGC(1/-1)
BspMI         719  ACCTGC               ACCTGC(4/8)
BstXI         375  CCATGGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         492  GCTGAGC              GC^T[ACGT]AGC
DpnII         750  GATC                 ^GATC
EaeI          704  CGGCCG               [CT]^GGCC[GA]
EagI          704  CGGCCG               C^GGCCG
EclXI         704  CGGCCG               C^GGCCG
Eco52I        704  CGGCCG               C^GGCCG
KpnI           26  GGTACC               GGTAC^C
MaeI          793  CTAG                 C^TAG
MaeIII        480  GTCAC                ^GT[ACGT]AC
MboI          750  GATC                 ^GATC
MfeI          764  CAATTG               C^AATTG
MseI          113  TTAA                 T^TAA
MspA1I         31  CAGCTG               C[AC]G^C[GT]G
MunI          764  CAATTG               C^AATTG
NdeII         750  GATC                 ^GATC
NheI          792  GCTAGC               G^CTAGC
PleI          232  GAGTC                GAGTC(4/5)
PstI          541  CTGCAG               CTGCA^G
PvuII          31  CAGCTG               CAG^CTG
Sau3AI        750  GATC                 ^GATC
SfcI          541  CTGCAG               C^T[GA][CT]AG
SmlI          724  CTCAAG               C^T[CT][GA]AG
TfiI          158  GATTC                G^A[AT]TC
Tru9I         113  TTAA                 T^TAA
Tsp45I        480  GTCAC                ^GT[GC]AC
TspRI         314  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AciI          366  CCGC                 CCGC(-3/-1)
              509  GCGG                
AspHI         172  GAGCTC               G[AT]GC[AT]^C
              265  GAGCTC              
AvaI          167  CTCGGG               C^[CT]CG[GA]G
              532  CCCGAG              
BanII         172  GAGCTC               G[GA]GC[CT]^C
              265  GAGCTC              
BmyI          172  GAGCTC               G[AGT]GC[ACT]^C
              265  GAGCTC              
BpmI          229  CTGGAG               CTGGAG(16/14)
              579  CTGGAG              
BsiHKAI       172  GAGCTC               G[AT]GC[AT]^C
              265  GAGCTC              
BsmFI          55  GGGAC                GGGAC(10/14)
              398  GGGAC               
BsoBI         167  CTCGGG               C^[CT]CG[GA]G
              532  CCCGAG              
Bsp1286I      172  GAGCTC               G[AGT]GC[ACT]^C
              265  GAGCTC              
DsaI          375  CCATGG               C^C[GA][CT]GG
              787  CCATGG              
EarI          340  CTCTTC               CTCTTC(1/4)
              142  GAAGAG              
HinfI         158  GATTC                G^A[ACGT]TC
              232  GAGTC               
HphI          258  GGTGA                GGTGA(8/7)
              571  GGTGA               
Ksp632I       340  CTCTTC               CTCTTC(1/4)
              142  GAAGAG              
NcoI          375  CCATGG               C^CATGG
              787  CCATGG              
SacI          172  GAGCTC               GAGCT^C
              265  GAGCTC              
Tsp509I       111  AATT                 ^AATT
              765  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AfaI        AluI        AvaII       BbvI        BcnI        
BsaJI       BsiYI       BslI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DraII       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HpaII       ItaI        MboII       
MnlI        MslI        MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       PpuMI       RsaI        
Sau96I      ScrFI       SfaNI       StyI        TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com