Restriction map for pF1KE0931
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BanI        
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshBI       PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AciI          624  CCGC                 CCGC(-3/-1)
Asp700I       298  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         440  GAGCAC               G[AT]GC[AT]^C
AspI          922  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          125  CCCGGG               C^[CT]CG[GA]G
AvaII         186  GGACC                G^G[AT]CC
BalI          990  TGGCCA               TGG^CCA
BanII         712  GAGCCC               G[GA]GC[CT]^C
BcgI          633  CGAGGAAAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI          272  CTCCAG               CTGGAG(16/14)
BsiHKAI       440  GAGCAC               G[AT]GC[AT]^C
BsoBI         125  CCCGGG               C^[CT]CG[GA]G
BspHI         902  TCATGA               T^CATGA
BstBI         551  TTCGAA               TT^CGAA
DsaI           18  CCGTGG               C^C[GA][CT]GG
EaeI          990  TGGCCA               [CT]^GGCC[GA]
EcoNI         987  CCTTGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         580  GATATC               GAT^ATC
EcoT14I       987  CCTTGG               C^C[AT][AT]GG
FauI          623  CCCGC                CCCGC(4/6)
HapII         126  CCGG                 C^CGG
HpaII         126  CCGG                 C^CGG
MluNI         990  TGGCCA               TGG^CCA
MscI          990  TGGCCA               TGG^CCA
MslI          897  CACAGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        624  CCGCTG               C[AC]G^C[GT]G
MspI          126  CCGG                 C^CGG
NspI          618  ACATGC               [GA]CATG^[CT]
NspV          551  TTCGAA               TT^CGAA
PflFI         922  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          945  GACTC                GAGTC(4/5)
PpuMI         185  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         945  GACTCTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          827  CTGCAG               CTGCA^G
RcaI          902  TCATGA               T^CATGA
SexAI         362  ACCTGGT              A^CC[AT]GGT
SfuI          551  TTCGAA               TT^CGAA
SmaI          125  CCCGGG               CCC^GGG
StuI          164  AGGCCT               AGG^CCT
StyI          987  CCTTGG               C^C[AT][AT]GG
Tth111I       922  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          773  CCAGACAGCCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         125  CCCGGG               C^CCGGG
XmaI          125  CCCGGG               C^CCGGG
XmnI          298  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AclI          390  AACGTT               AA^CGTT
              793  AACGTT              
AfaI           79  GTAC                 GT^AC
              892  GTAC                
BcnI          125  CCCGG                CC^[GC]GG
              126  CCGGG               
BfaI          742  CTAG                 C^TAG
              910  CTAG                
BmyI          440  GAGCAC               G[AGT]GC[ACT]^C
              712  GAGCCC              
BsmFI         277  GGGAC                GGGAC(10/14)
              331  GGGAC               
Bsp1286I      440  GAGCAC               G[AGT]GC[ACT]^C
              712  GAGCCC              
BsrI          484  ACTGG                ACTGG(1/-1)
              558  ACTGG               
BstF5I        612  CATCC                GGATG(2/0)
             1038  GGATG               
CfoI           33  GCGC                 GCG^C
              807  GCGC                
DraII          70  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              185  AGGACCT             
DraIII        349  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
              965  CACATGGTG           
EarI          174  GAAGAG               CTCTTC(1/4)
             1108  GAAGAG              
EcoO109I       70  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              185  AGGACCT             
FokI          612  CATCC                GGATG(9/13)
             1038  GGATG               
HaeII          32  GGCGCT               [GA]GCGC^[CT]
              806  GGCGCT              
HhaI           33  GCGC                 GCG^C
              807  GCGC                
HinP1I         33  GCGC                 G^CGC
              807  GCGC                
Ksp632I       174  GAAGAG               CTCTTC(1/4)
             1108  GAAGAG              
MaeI          742  CTAG                 C^TAG
              910  CTAG                
MaeII         391  ACGT                 A^CGT
              794  ACGT                
MseI          463  TTAA                 T^TAA
              659  TTAA                
NciI          125  CCCGG                CC^[GC]GG
              126  CCGGG               
Psp1406I      390  AACGTT               AA^CGTT
              793  AACGTT              
RsaI           79  GTAC                 GT^AC
              892  GTAC                
Tru9I         463  TTAA                 T^TAA
              659  TTAA                
Tsp45I          8  GTGAC                ^GT[GC]AC
              232  GTGAC               
Tsp509I       199  AATT                 ^AATT
              294  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AluI        AlwI        AlwNI       BamHI       BbvI        
BsaJI       BseRI       BsiYI       BslI        BssKI       
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      HaeIII      HinfI       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TaqI        TfiI        
TseI        TspRI       TthHB8I     XhoII       
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