Restriction map for pF1KE0927
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiHKAI     BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DraIII      Eam1105I    Eco47III    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        KspI        MaeIII      MamI        MfeI        
MluI        MroI        MspA1I      MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SspBI       SspI        SwaI        TfiI        
Tsp45I      Tsp509I     Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII         216  CGCG                 CG^CG
AcyI            9  GACGCC               G[GA]^CG[CT]C
AflIII        588  ACATGT               A^C[GA][CT]GT
BamHI          58  GGATCC               G^GATCC
BanI          808  GGTGCC               G^G[CT][GA]CC
BanII         654  GGGCTC               G[GA]GC[CT]^C
BbsI          730  GAAGAC               GAAGAC(2/6)
BglII         779  AGATCT               A^GATCT
BmyI          654  GGGCTC               G[AGT]GC[ACT]^C
BpmI          548  CTGGAG               CTGGAG(16/14)
BpuAI         730  GAAGAC               GAAGAC(2/6)
BsaHI           9  GACGCC               G[GA]^CG[CT]C
BsaI          556  GAGACC               GGTCTC(1/5)
BsiEI         659  CGGCCG               CG[GA][CT]^CG
BsmI          623  GCATTC               GAATGC(1/-1)
Bsp1286I      654  GGGCTC               G[AGT]GC[ACT]^C
BspLU11I      588  ACATGT               A^CATGT
BstUI         216  CGCG                 CG^CG
CfoI          465  GCGC                 GCG^C
DraI          964  TTTAAA               TTT^AAA
DrdI          427  GACTTCGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          527  CCGTGG               C^C[GA][CT]GG
EagI          659  CGGCCG               C^GGCCG
EclXI         659  CGGCCG               C^GGCCG
Eco52I        659  CGGCCG               C^GGCCG
EcoNI         408  CCTGCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       488  CCAAGG               C^C[AT][AT]GG
HgaI            9  GACGC                GACGC(5/10)
HhaI          465  GCGC                 GCG^C
Hin1I           9  GACGCC               G[GA]^CG[CT]C
HinP1I        465  GCGC                 G^CGC
MaeII         143  ACGT                 A^CGT
MseI          965  TTAA                 T^TAA
MslI          147  CATTGGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          216  CGCG                 CG^CG
PflMI         720  CCACACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         365  GGGACCT              [GA]G^G[AT]CC[CT]
SapI          615  GAAGAGC              GCTCTTC(1/4)
SbfI          408  CCTGCAGG             CCTGCA^GG
SfaNI        1056  GATGC                GCATC(5/9)
SmlI          691  CTCAAG               C^T[CT][GA]AG
Sse8387I      408  CCTGCAGG             CCTGCA^GG
StuI          596  AGGCCT               AGG^CCT
StyI          488  CCAAGG               C^C[AT][AT]GG
Tru9I         965  TTAA                 T^TAA
Van91I        720  CCACACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwNI         403  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1067  CAGCCTCTG           
BalI          185  TGGCCA               TGG^CCA
              320  TGGCCA              
BseRI          96  GAGGAG               GAGGAG(10/8)
              958  GAGGAG              
BsmAI         425  GAGAC                GTCTC(1/5)
              556  GAGAC               
BspMI         314  ACCTGC               ACCTGC(4/8)
             1034  ACCTGC              
BsrI          479  ACTGG                ACTGG(1/-1)
              805  ACTGG               
BstYI          58  GGATCC               [GA]^GATC[CT]
              779  AGATCT              
EarI          615  GAAGAG               CTCTTC(1/4)
              955  GAAGAG              
HphI          167  TCACC                GGTGA(8/7)
              627  TCACC               
Ksp632I       615  GAAGAG               CTCTTC(1/4)
              955  GAAGAG              
MflI           58  GGATCC               [GA]^GATC[CT]
              779  AGATCT              
MluNI         185  TGGCCA               TGG^CCA
              320  TGGCCA              
MscI          185  TGGCCA               TGG^CCA
              320  TGGCCA              
PstI          409  CTGCAG               CTGCA^G
             1073  CTGCAG              
SfcI          409  CTGCAG               C^T[GA][CT]AG
             1073  CTGCAG              
SphI          461  GCATGC               GCATG^C
              831  GCATGC              
TspRI         804  CACTG                CA[GC]TG[ACGT][ACGT]^
              915  CACTG               
XhoII          58  GGATCC               [GA]^GATC[CT]
              779  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BcnI        BfaI        BsaJI       BsiYI       
BslI        BsmFI       BsrBI       BssKI       BstF5I      
BstNI       BstXI       Cac8I       Cfr13I      DpnII       
DraII       EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        MaeI        MboI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        RsaI        Sau3AI      
Sau96I      ScrFI       TaqI        TseI        TthHB8I     
XcmI        
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