Restriction map for pF1KE0924
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflIII      AgeI        AhdI        Aor51HI     ApaI        
AscI        AseI        AsnI        AspEI       AspI        
AviII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BglI        BglII       BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstF5I      BstUI       BstXI       
BstZ17I     CelII       CfoI        ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoRV       
EcoT22I     FbaI        FokI        FspI        HaeII       
HhaI        HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KspI        MaeII       MamI        
MfeI        MluI        MroI        MslI        MunI        
MvnI        NarI        NcoI        NdeI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        466  GGTACC               G^GTACC
AcsI          909  GAATTC               [GA]^AATT[CT]
AcyI          650  GGCGTC               G[GA]^CG[CT]C
AflII         115  CTTAAG               C^TTAAG
Alw44I        390  GTGCAC               G^TGCAC
AocI          604  CCTGAGG              CC^T[ACGT]AGG
ApaLI         390  GTGCAC               G^TGCAC
ApoI          909  GAATTC               [GA]^AATT[CT]
Asp700I       534  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       466  GGTACC               G^GTACC
AspHI         390  GTGCAC               G[AT]GC[AT]^C
AvaI          461  CCCGGG               C^[CT]CG[GA]G
AvrII         147  CCTAGG               C^CTAGG
BalI          965  TGGCCA               TGG^CCA
BamHI         110  GGATCC               G^GATCC
BanI          466  GGTACC               G^G[CT][GA]CC
BanII          16  GGGCTC               G[GA]GC[CT]^C
BfrI          115  CTTAAG               C^TTAAG
BlnI          147  CCTAGG               C^CTAGG
BsaHI         650  GGCGTC               G[GA]^CG[CT]C
BsaWI         488  ACCGGA               [AT]^CCGG[AT]
BseRI         339  CTCCTC               GAGGAG(10/8)
BsgI          740  CTGCAC               GTGCAG(16/14)
BsiHKAI       390  GTGCAC               G[AT]GC[AT]^C
BsmI           86  GAATGC               GAATGC(1/-1)
BsoBI         461  CCCGGG               C^[CT]CG[GA]G
BstAPI         60  GCAGATTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        404  GGTCACC              G^GT[ACGT]ACC
BstPI         404  GGTCACC              G^GT[ACGT]ACC
Bsu36I        604  CCTGAGG              CC^T[ACGT]AGG
EarI          638  GAAGAG               CTCTTC(1/4)
Eco81I        604  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       404  GGTCACC              G^GT[ACGT]ACC
EcoRI         909  GAATTC               G^AATTC
FauI          360  GCGGG                CCCGC(4/6)
FseI           31  GGCCGGCC             GGCCGG^CC
HgaI          651  GCGTC                GACGC(5/10)
Hin1I         650  GGCGTC               G[GA]^CG[CT]C
KpnI          466  GGTACC               GGTAC^C
Ksp632I       638  GAAGAG               CTCTTC(1/4)
MluNI         965  TGGCCA               TGG^CCA
MscI          965  TGGCCA               TGG^CCA
MseI          116  TTAA                 T^TAA
NaeI           32  GCCGGC               GCC^GGC
NgoMIV         32  GCCGGC               G^CCGGC
NheI           72  GCTAGC               G^CTAGC
PpuMI         798  GGGTCCC              [GA]G^G[AT]CC[CT]
SapI          638  GAAGAGC              GCTCTTC(1/4)
SmaI          461  CCCGGG               CCC^GGG
TaqI          234  TCGA                 T^CGA
Tru9I         116  TTAA                 T^TAA
TthHB8I       234  TCGA                 T^CGA
XmaCI         461  CCCGGG               C^CCGGG
XmaI          461  CCCGGG               C^CCGGG
XmnI          534  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          467  GTAC                 GT^AC
              712  GTAC                
AlwNI         700  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              985  CAGGCCCTG           
BciVI          48  GGATAC               GTATCC(6/5)
              555  GTATCC              
BfaI           73  CTAG                 C^TAG
              148  CTAG                
BpmI          422  CTCCAG               CTGGAG(16/14)
              529  CTGGAG              
BsrFI          32  GCCGGC               [GA]^CCGG[CT]
              481  ACCGGC              
BstYI         110  GGATCC               [GA]^GATC[CT]
              143  AGATCC              
Cfr10I         32  GCCGGC               [GA]^CCGG[CT]
              481  ACCGGC              
DpnII         111  GATC                 ^GATC
              144  GATC                
DraII         798  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              986  AGGCCCT             
DsaI          205  CCACGG               C^C[GA][CT]GG
              573  CCGTGG              
EcoO109I      798  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              986  AGGCCCT             
EcoT14I       147  CCTAGG               C^C[AT][AT]GG
              715  CCAAGG              
MaeI           73  CTAG                 C^TAG
              148  CTAG                
MboI          111  GATC                 ^GATC
              144  GATC                
MboII         638  GAAGA                GAAGA(8/7)
              503  TCTTC               
MflI          110  GGATCC               [GA]^GATC[CT]
              143  AGATCC              
NdeII         111  GATC                 ^GATC
              144  GATC                
RsaI          467  GTAC                 GT^AC
              712  GTAC                
Sau3AI        111  GATC                 ^GATC
              144  GATC                
StyI          147  CCTAGG               C^C[AT][AT]GG
              715  CCAAGG              
Tsp509I       237  AATT                 ^AATT
              910  AATT                
XhoII         110  GGATCC               [GA]^GATC[CT]
              143  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstNI       Cac8I       Cfr13I      DdeI        EaeI        
EcoRII      Fnu4HI      HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeIII      MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NlaIV       PleI        PvuII       Sau96I      ScrFI       
SmlI        TfiI        TseI        Tsp45I      TspRI       
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