Restriction map for
pF1KE0924
(Circular) (Six-base) MAPSORT of: pF1KE0924.seq Check: 3479 from: 1 to: 4142
pF1KE0924 4142 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1180 1180
Size: 4142
Acc65I G'GTAC_C
Cuts at: 574 1158 574
Size: 584 3558
AclI AA'CG_TT
Cuts at: 3982 3982
Size: 4142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 431 1967 2399 3146 3414 376
Size: 55 1536 432 747 268 1104
Fragments arranged by size:
1536 1104 747 432 268 55
AflII C'TTAA_G
Cuts at: 223 223
Size: 4142
AflIII A'CryG_T
Cuts at: 1494 2598 3575 1494
Size: 1104 977 2061
Fragments arranged by size:
2061 1104 977
AgeI A'CCGG_T
Cuts at: 2530 2530
Size: 4142
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 631 663 631
Size: 32 4110
AlwNI CAG_nnn'CTG
Cuts at: 813 1098 1447 3014 813
Size: 285 349 1567 1941
Fragments arranged by size:
1941 1567 349 285
ApaLI G'TGCA_C
Cuts at: 498 2912 498
Size: 2414 1728
ApoI r'AATT_y
Cuts at: 1017 1146 3495 3751 1017
Size: 129 2349 256 1408
Fragments arranged by size:
2349 1408 256 129
AseI AT'TA_AT
Cuts at: 20 20
Size: 4142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
AvaI C'yCGr_G
Cuts at: 569 1162 569
Size: 593 3549
AvrII C'CTAG_G
Cuts at: 255 255
Size: 4142
BamHI G'GATC_C
Cuts at: 218 1167 218
Size: 949 3193
BanI G'GyrC_C
Cuts at: 574 1158 1572 1820 1855 574
Size: 584 414 248 35 2861
Fragments arranged by size:
2861 584 414 248 35
BanII G_rGCy'C
Cuts at: 128 1156 2186 128
Size: 1028 1030 2084
Fragments arranged by size:
2084 1030 1028
BbeI G_GCGC'C
Cuts at: 1576 1824 1576
Size: 248 3894
BciVI GTATCCnnnnn_n'
Cuts at: 150 674 2040 2801 4142 150
Size: 524 1366 761 1341 150
Fragments arranged by size:
1366 1341 761 524 150
BclI T'GATC_A
Cuts at: 1662 1662
Size: 4142
BglII A'GATC_T
Cuts at: 1657 3694 1657
Size: 2037 2105
BlpI GC'TnA_GC
Cuts at: 1254 1254
Size: 4142
Bme1580I G_kGCm'C
Cuts at: 502 875 1767 1860 2916 502
Size: 373 892 93 1056 1728
Fragments arranged by size:
1728 1056 892 373 93
BmrI ACTGGGnnnn_n'
Cuts at: 434 913 1354 1435 1770 3914 434
Size: 479 441 81 335 2144 662
Fragments arranged by size:
2144 662 479 441 335 81
BmtI G_CTAG'C
Cuts at: 184 184
Size: 4142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 4142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 658 515
Size: 143 3999
Bpu10I CC'TnA_GC
Cuts at: 508 508
Size: 4142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 967 1318 2197 2689 2987 760
Size: 207 351 879 492 298 1915
Fragments arranged by size:
1915 879 492 351 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 3846 3846
Size: 4142
BsaAI yAC'GTr
Cuts at: 1495 1495
Size: 4142
BsaBI GATnn'nnATC
Cuts at: 6 1656 3693 6
Size: 1650 2037 455
Fragments arranged by size:
2037 1650 455
BsaHI Gr'CG_yC
Cuts at: 759 1573 1821 2523 4019 759
Size: 814 248 702 1496 882
Fragments arranged by size:
1496 882 814 702 248
BsaWI w'CCGG_w
Cuts at: 596 1331 1852 2530 2804 2951 3362 596
Size: 735 521 678 274 147 411 1376
Fragments arranged by size:
1376 735 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 631 661 631
Size: 30 4112
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 438 438
Size: 4142
BseYI C'CCAG_C
Cuts at: 350 929 992 1567 2325 2902 3398 350
Size: 579 63 575 758 577 496 1094
Fragments arranged by size:
1094 758 579 577 575 496 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 833
Size: 4142
BsiEI CG_ry'CG
Cuts at: 104 1684 1730 2938 104
Size: 1580 46 1208 1308
Fragments arranged by size:
1580 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 502 1156 1934 2124 2916 502
Size: 654 778 190 792 1728
Fragments arranged by size:
1728 792 778 654 190
BsmI GAATG_Cn'
Cuts at: 200 200
Size: 4142
Bsp1286I G_dGCh'C
Cuts at: 128 502 875 1156 1767 1860 1934 2124 2186
Size: 374 373 281 611 93 74 190 62
Cuts at: 2186 2916 128
Size: 730 1354
Fragments arranged by size:
1354 730 611 374 373 281 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3362 3362
Size: 4142
BspHI T'CATG_A
Cuts at: 3318 3318
Size: 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1192 1406 1708 2089 1192
Size: 214 302 381 3245
Fragments arranged by size:
3245 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2434 2488 3972 4137 50
Size: 2384 54 1484 165 55
Fragments arranged by size:
2384 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2054 2054
Size: 4142
BsrFI r'CCGG_y
Cuts at: 140 589 2140 2321 2530 140
Size: 449 1551 181 209 1752
Fragments arranged by size:
1752 1551 449 209 181
BssSI C'ACGA_G
Cuts at: 2413 2771 2413
Size: 358 3784
BstAPI GCAn_nnn'nTGC
Cuts at: 174 1519 174
Size: 1345 2797
BstBI TT'CG_AA
Cuts at: 2503 2503
Size: 4142
BstEII G'GTnAC_C
Cuts at: 512 512
Size: 4142
BstYI r'GATC_y
Cuts at: 1 218 251 1167 1634 1657 1992 2238 3250
Size: 217 33 916 467 23 335 246 1012
Cuts at: 3250 3336 3348 3694 1
Size: 86 12 346 449
Fragments arranged by size:
1012 916 467 449 346 335 246 217
86 33 23 12
Bsu36I CC'TnA_GG
Cuts at: 713 713
Size: 4142
BtgI C'CryG_G
Cuts at: 313 681 313
Size: 368 3774
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2092 2273 2436 2092
Size: 181 163 3798
Fragments arranged by size:
3798 181 163
BtsI GCAGTG_nn'
Cuts at: 873 1385 873
Size: 512 3630
DraI TTT'AAA
Cuts at: 1141 1141
Size: 4142
DrdI GACnn_nn'nnGTC
Cuts at: 1848 2706 1848
Size: 858 3284
EaeI y'GGCC_r
Cuts at: 138 591 1073 1727 1901 2292 2319 3664 138
Size: 453 482 654 174 391 27 1345 616
Fragments arranged by size:
1345 654 616 482 453 391 174 27
EagI C'GGCC_G
Cuts at: 1727 1727
Size: 4142
EarI CTCTTCn'nnn_
Cuts at: 741 2165 2375 741
Size: 1424 210 2508
Fragments arranged by size:
2508 1424 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2350 2660 2806 3952 2350
Size: 310 146 1146 2540
Fragments arranged by size:
2540 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1154 1154
Size: 4142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 431 515 658 1967 2399 3146 3414 376
Size: 55 84 143 1309 432 747 268 1104
Fragments arranged by size:
1309 1104 747 432 268 143 84 55
EcoO109I rG'GnC_Cy
Cuts at: 907 1095 1281 907
Size: 188 186 3768
Fragments arranged by size:
3768 188 186
EcoRI G'AATT_C
Cuts at: 1017 1146 1017
Size: 129 4013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 4142
FseI GG_CCGG'CC
Cuts at: 144 144
Size: 4142
FspI TGC'GCA
Cuts at: 1923 1923
Size: 4142
HaeII r_GCGC'y
Cuts at: 1576 1824 2846 3825 1576
Size: 248 1022 979 1893
Fragments arranged by size:
1893 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1984 2014 2016 2046 1984
Size: 30 2 30 4080
Fragments arranged by size:
4080 30 30 2
HincII GTy'rAC
Cuts at: 1181 1181
Size: 4142
KasI G'GCGC_C
Cuts at: 1572 1820 1572
Size: 248 3894
KpnI G_GTAC'C
Cuts at: 578 1162 578
Size: 584 3558
MluI A'CGCG_T
Cuts at: 3575 3575
Size: 4142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 417 764 2813 2997 417
Size: 347 2049 184 1562
Fragments arranged by size:
2049 1562 347 184
MscI TGG'CCA
Cuts at: 1075 1903 1075
Size: 828 3314
MslI CAynn'nnrTG
Cuts at: 2131 2258 2131
Size: 127 4015
MspA1I CmG'CkG
Cuts at: 354 520 535 792 924 1253 1567 1927 2940
Size: 166 15 257 132 329 314 360 1013
Cuts at: 2940 3185 354
Size: 245 1311
Fragments arranged by size:
1311 1013 360 329 314 257 245 166
132 15
NaeI GCC'GGC
Cuts at: 142 2323 142
Size: 2181 1961
NarI GG'CG_CC
Cuts at: 1573 1821 1573
Size: 248 3894
NgoMIV G'CCGG_C
Cuts at: 140 2321 140
Size: 2181 1961
NheI G'CTAG_C
Cuts at: 180 180
Size: 4142
NspI r_CATG'y
Cuts at: 1195 1695 2602 1195
Size: 500 907 2735
Fragments arranged by size:
2735 907 500
PasI CC'CwG_GG
Cuts at: 583 807 583
Size: 224 3918
PciI A'CATG_T
Cuts at: 2598 2598
Size: 4142
PmeI GTTT'AAAC
Cuts at: 1141 1141
Size: 4142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 631 663 3281 3313 631
Size: 32 2618 32 1460
Fragments arranged by size:
2618 1460 32 32
PpuMI rG'GwC_Cy
Cuts at: 907 907
Size: 4142
PstI C_TGCA'G
Cuts at: 1189 1874 1189
Size: 685 3457
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4142
PvuII CAG'CTG
Cuts at: 354 520 535 792 924 1567 1927 354
Size: 166 15 257 132 643 360 2569
Fragments arranged by size:
2569 643 360 257 166 132 15
RsrII CG'GwC_CG
Cuts at: 2337 2337
Size: 4142
SacI G_AGCT'C
Cuts at: 1156 1156
Size: 4142
SalI G'TCGA_C
Cuts at: 1179 1179
Size: 4142
SanDI GG'GwC_CC
Cuts at: 907 907
Size: 4142
SapI GCTCTTCn'nnn_
Cuts at: 741 2165 2375 741
Size: 1424 210 2508
Fragments arranged by size:
2508 1424 210
SbfI CC_TGCA'GG
Cuts at: 1189 1189
Size: 4142
ScaI AGT'ACT
Cuts at: 3659 3659
Size: 4142
SfcI C'TryA_G
Cuts at: 33 1185 1870 2863 3054 33
Size: 1152 685 993 191 1121
Fragments arranged by size:
1152 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1574 1822 1574
Size: 248 3894
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
SmaI CCC'GGG
Cuts at: 571 1164 571
Size: 593 3549
SmlI C'TyrA_G
Cuts at: 223 775 982 1297 2212 2704 2966 223
Size: 552 207 315 915 492 262 1399
Fragments arranged by size:
1399 915 552 492 315 262 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4142
SphI G_CATG'C
Cuts at: 1195 1695 1195
Size: 500 3642
StyI C'CwwG_G
Cuts at: 255 823 1276 1630 255
Size: 568 453 354 2767
Fragments arranged by size:
2767 568 453 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 439 596 1725 1858 2317 2526 439
Size: 157 1129 133 459 209 2055
Fragments arranged by size:
2055 1129 459 209 157 133
TatI w'GTAC_w
Cuts at: 3657 3657
Size: 4142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3659 3691 3659
Size: 32 4110
Tth111I GACn'n_nGTC
Cuts at: 1939 1939
Size: 4142
XbaI T'CTAG_A
Cuts at: 1173 1173
Size: 4142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1637 1637
Size: 4142
XmaI C'CCGG_G
Cuts at: 569 1162 569
Size: 593 3549
XmnI GAAnn'nnTTC
Cuts at: 646 646
Size: 4142
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PasI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BsiWI
BsmBI BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FspAI HindIII HpaI MfeI NcoI NdeI
NotI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI