Restriction map for pF1KE0924
 (Linear) (Six-base) MAPSORT of: KIEE0924.seq  Check: 1987  from: 1  to: 1029



KIEE0924.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

Acc65I G'GTAC_C

Cuts at:      0     466    1029 
   Size:        466     563
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     323    1029 
   Size:        268      55     706

  Fragments arranged by size: 

                 706     268      55
 
AflII C'TTAA_G

Cuts at:      0     115    1029 
   Size:        115     914
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     523     555    1029 
   Size:        523      32     474

  Fragments arranged by size: 

                 523     474      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     705     990    1029 
   Size:        705     285      39

  Fragments arranged by size: 

                 705     285      39
 
ApaLI G'TGCA_C

Cuts at:      0     390    1029 
   Size:        390     639
 
ApoI r'AATT_y

Cuts at:      0     909    1029 
   Size:        909     120
 
AvaI C'yCGr_G

Cuts at:      0     461    1029 
   Size:        461     568
 
AvrII C'CTAG_G

Cuts at:      0     147    1029 
   Size:        147     882
 
BamHI G'GATC_C

Cuts at:      0     110    1029 
   Size:        110     919
 
BanI G'GyrC_C

Cuts at:      0     466    1029 
   Size:        466     563
 
BanII G_rGCy'C

Cuts at:      0      20    1029 
   Size:         20    1009
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      42     566    1029 
   Size:         42     524     463

  Fragments arranged by size: 

                 524     463      42
 
Bme1580I G_kGCm'C

Cuts at:      0     394     767    1029 
   Size:        394     373     262

  Fragments arranged by size: 

                 394     373     262
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     326     805    1029 
   Size:        326     479     224

  Fragments arranged by size: 

                 479     326     224
 
BmtI G_CTAG'C

Cuts at:      0      76    1029 
   Size:         76     953
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407     550    1029 
   Size:        407     143     479

  Fragments arranged by size: 

                 479     407     143
 
Bpu10I CC'TnA_GC

Cuts at:      0     400    1029 
   Size:        400     629
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652     859    1029 
   Size:        652     207     170

  Fragments arranged by size: 

                 652     207     170
 
BsaHI Gr'CG_yC

Cuts at:      0     651    1029 
   Size:        651     378
 
BsaWI w'CCGG_w

Cuts at:      0     488    1029 
   Size:        488     541
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     523     553    1029 
   Size:        523      30     476

  Fragments arranged by size: 

                 523     476      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     330    1029 
   Size:        330     699
 
BseYI C'CCAG_C

Cuts at:      0     242     821     884    1029 
   Size:        242     579      63     145

  Fragments arranged by size: 

                 579     242     145      63
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     725    1029 
   Size:        725     304
 
BsiHKAI G_wGCw'C

Cuts at:      0     394    1029 
   Size:        394     635
 
BsmI GAATG_Cn'

Cuts at:      0      92    1029 
   Size:         92     937
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     394     767    1029 
   Size:         20     374     373     262

  Fragments arranged by size: 

                 374     373     262      20
 
BsrFI r'CCGG_y

Cuts at:      0      32     481    1029 
   Size:         32     449     548

  Fragments arranged by size: 

                 548     449      32
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      66    1029 
   Size:         66     963
 
BstEII G'GTnAC_C

Cuts at:      0     404    1029 
   Size:        404     625
 
BstYI r'GATC_y

Cuts at:      0     110     143    1029 
   Size:        110      33     886

  Fragments arranged by size: 

                 886     110      33
 
Bsu36I CC'TnA_GG

Cuts at:      0     605    1029 
   Size:        605     424
 
BtgI C'CryG_G

Cuts at:      0     205     573    1029 
   Size:        205     368     456

  Fragments arranged by size: 

                 456     368     205
 
BtsI GCAGTG_nn'

Cuts at:      0     765    1029 
   Size:        765     264
 
EaeI y'GGCC_r

Cuts at:      0      30     483     965    1029 
   Size:         30     453     482      64

  Fragments arranged by size: 

                 482     453      64      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     633    1029 
   Size:        633     396
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     323     407     550    1029 
   Size:        268      55      84     143     479

  Fragments arranged by size: 

                 479     268     143      84      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0     799     987    1029 
   Size:        799     188      42

  Fragments arranged by size: 

                 799     188      42
 
EcoRI G'AATT_C

Cuts at:      0     909    1029 
   Size:        909     120
 
FseI GG_CCGG'CC

Cuts at:      0      36    1029 
   Size:         36     993
 
KpnI G_GTAC'C

Cuts at:      0     470    1029 
   Size:        470     559
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     309     656    1029 
   Size:        309     347     373

  Fragments arranged by size: 

                 373     347     309
 
MscI TGG'CCA

Cuts at:      0     967    1029 
   Size:        967      62
 
MspA1I CmG'CkG

Cuts at:      0     246     412     427     684     816    1029 
   Size:        246     166      15     257     132     213

  Fragments arranged by size: 

                 257     246     213     166     132      15
 
NaeI GCC'GGC

Cuts at:      0      34    1029 
   Size:         34     995
 
NgoMIV G'CCGG_C

Cuts at:      0      32    1029 
   Size:         32     997
 
NheI G'CTAG_C

Cuts at:      0      72    1029 
   Size:         72     957
 
PasI CC'CwG_GG

Cuts at:      0     475     699    1029 
   Size:        475     224     330

  Fragments arranged by size: 

                 475     330     224
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     523     555    1029 
   Size:        523      32     474

  Fragments arranged by size: 

                 523     474      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     799    1029 
   Size:        799     230
 
PvuII CAG'CTG

Cuts at:      0     246     412     427     684     816    1029 
   Size:        246     166      15     257     132     213

  Fragments arranged by size: 

                 257     246     213     166     132      15
 
SanDI GG'GwC_CC

Cuts at:      0     799    1029 
   Size:        799     230
 
SapI GCTCTTCn'nnn_

Cuts at:      0     633    1029 
   Size:        633     396
 
SmaI CCC'GGG

Cuts at:      0     463    1029 
   Size:        463     566
 
SmlI C'TyrA_G

Cuts at:      0     115     667     874    1029 
   Size:        115     552     207     155

  Fragments arranged by size: 

                 552     207     155     115
 
StyI C'CwwG_G

Cuts at:      0     147     715    1029 
   Size:        147     568     314

  Fragments arranged by size: 

                 568     314     147
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     331     488    1029 
   Size:        331     157     541

  Fragments arranged by size: 

                 541     331     157
 
XmaI C'CCGG_G

Cuts at:      0     461    1029 
   Size:        461     568
 
XmnI GAAnn'nnTTC

Cuts at:      0     538    1029 
   Size:        538     491
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII     AloI    AlwNI    ApaLI     ApoI     AvaI
    AvrII    BamHI     BanI    BanII    BciVI Bme1580I     BmrI     BmtI
     BpmI   Bpu10I    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BsrFI   BstAPI   BstEII    BstYI
   Bsu36I     BtgI     BtsI     EaeI     EarI  Eco57MI EcoO109I    EcoRI
     FseI     KpnI     MmeI     MscI   MspA1I     NaeI   NgoMIV     NheI
     PasI     PpiI    PpuMI    PvuII    SanDI     SapI     SmaI     SmlI
     StyI    TaqII     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI     AscI     AseI    AsiSI     BaeI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BplI     BsaI    BsaAI    BsaBI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
    BstBI    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MslI     NarI     NcoI     NdeI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI
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