Restriction map for pF1KE0919
 (Linear) (Six-base) MAPSORT of: KIEE0919.seq  Check: 1005  from: 1  to: 915



KIEE0919.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     607     621     792     915 
   Size:        607      14     171     123

  Fragments arranged by size: 

                 607     171     123      14
 
AflIII A'CryG_T

Cuts at:      0     332     915 
   Size:        332     583
 
AgeI A'CCGG_T

Cuts at:      0     698     915 
   Size:        698     217
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     182     915 
   Size:        182     733
 
ApaI G_GGCC'C

Cuts at:      0      73     218     915 
   Size:         73     145     697

  Fragments arranged by size: 

                 697     145      73
 
ApaLI G'TGCA_C

Cuts at:      0     355     915 
   Size:        355     560
 
ApoI r'AATT_y

Cuts at:      0     430     915 
   Size:        430     485
 
AvaI C'yCGr_G

Cuts at:      0      65     580     915 
   Size:         65     515     335

  Fragments arranged by size: 

                 515     335      65
 
BanI G'GyrC_C

Cuts at:      0     243     915 
   Size:        243     672
 
BanII G_rGCy'C

Cuts at:      0      73     218     580     885     915 
   Size:         73     145     362     305      30

  Fragments arranged by size: 

                 362     305     145      73      30
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      42      76     915 
   Size:         42      34     839

  Fragments arranged by size: 

                 839      42      34
 
BclI T'GATC_A

Cuts at:      0     590     722     915 
   Size:        590     132     193

  Fragments arranged by size: 

                 590     193     132
 
BlpI GC'TnA_GC

Cuts at:      0     884     915 
   Size:        884      31
 
Bme1580I G_kGCm'C

Cuts at:      0      73     218     359     915 
   Size:         73     145     141     556

  Fragments arranged by size: 

                 556     145     141      73
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     546     802     915 
   Size:        331     215     256     113

  Fragments arranged by size: 

                 331     256     215     113
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     439     727     915 
   Size:        439     288     188

  Fragments arranged by size: 

                 439     288     188
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     564     823     915 
   Size:        564     259      92

  Fragments arranged by size: 

                 564     259      92
 
BsaAI yAC'GTr

Cuts at:      0     335     915 
   Size:        335     580
 
BsaWI w'CCGG_w

Cuts at:      0     698     915 
   Size:        698     217
 
BseYI C'CCAG_C

Cuts at:      0      81     915 
   Size:         81     834
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     915 
   Size:          2     913
 
BsiEI CG_ry'CG

Cuts at:      0      78     915 
   Size:         78     837
 
BsiHKAI G_wGCw'C

Cuts at:      0     359     878     915 
   Size:        359     519      37

  Fragments arranged by size: 

                 519     359      37
 
Bsp1286I G_dGCh'C

Cuts at:      0      73     218     359     580     878     885     915 
   Size:         73     145     141     221     298       7      30

  Fragments arranged by size: 

                 298     221     145     141      73      30       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     607     621     792     915 
   Size:        607      14     171     123

  Fragments arranged by size: 

                 607     171     123      14
 
BsrBI CCG'CTC

Cuts at:      0     708     915 
   Size:        708     207
 
BsrDI GCAATG_nn'

Cuts at:      0     842     915 
   Size:        842      73
 
BsrFI r'CCGG_y

Cuts at:      0     698     915 
   Size:        698     217
 
BstBI TT'CG_AA

Cuts at:      0     467     915 
   Size:        467     448
 
BstEII G'GTnAC_C

Cuts at:      0     807     915 
   Size:        807     108
 
BstYI r'GATC_y

Cuts at:      0     459     915 
   Size:        459     456
 
BtgI C'CryG_G

Cuts at:      0      35     250     404     915 
   Size:         35     215     154     511

  Fragments arranged by size: 

                 511     215     154      35
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     209     915 
   Size:        209     706
 
EaeI y'GGCC_r

Cuts at:      0      75     269     363     915 
   Size:         75     194      94     552

  Fragments arranged by size: 

                 552     194      94      75
 
EagI C'GGCC_G

Cuts at:      0      75     915 
   Size:         75     840
 
EarI CTCTTCn'nnn_

Cuts at:      0     469     915 
   Size:        469     446
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      21     403     915 
   Size:         21     382     512

  Fragments arranged by size: 

                 512     382      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     546     802     915 
   Size:        331     215     256     113

  Fragments arranged by size: 

                 331     256     215     113
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     191     915 
   Size:        191     724
 
EcoO109I rG'GnC_Cy

Cuts at:      0      69      70     214     324     475     915 
   Size:         69       1     144     110     151     440

  Fragments arranged by size: 

                 440     151     144     110      69       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     480     915 
   Size:        480     435
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     915 
   Size:        272     643
 
MscI TGG'CCA

Cuts at:      0     271     365     915 
   Size:        271      94     550

  Fragments arranged by size: 

                 550     271      94
 
MslI CAynn'nnrTG

Cuts at:      0     249     915 
   Size:        249     666
 
MspA1I CmG'CkG

Cuts at:      0     183     915 
   Size:        183     732
 
NcoI C'CATG_G

Cuts at:      0     250     915 
   Size:        250     665
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     284     915 
   Size:        284     631
 
PfoI T'CCnGG_A

Cuts at:      0     308     434     915 
   Size:        308     126     481

  Fragments arranged by size: 

                 481     308     126
 
PpuMI rG'GwC_Cy

Cuts at:      0     324     475     915 
   Size:        324     151     440

  Fragments arranged by size: 

                 440     324     151
 
PspOMI G'GGCC_C

Cuts at:      0      69     214     915 
   Size:         69     145     701

  Fragments arranged by size: 

                 701     145      69
 
PstI C_TGCA'G

Cuts at:      0     213     803     915 
   Size:        213     590     112

  Fragments arranged by size: 

                 590     213     112
 
SanDI GG'GwC_CC

Cuts at:      0     475     915 
   Size:        475     440
 
SbfI CC_TGCA'GG

Cuts at:      0     213     803     915 
   Size:        213     590     112

  Fragments arranged by size: 

                 590     213     112
 
SfcI C'TryA_G

Cuts at:      0     209     381     799     897     915 
   Size:        209     172     418      98      18

  Fragments arranged by size: 

                 418     209     172      98      18
 
SmaI CCC'GGG

Cuts at:      0      67     915 
   Size:         67     848
 
SmlI C'TyrA_G

Cuts at:      0     454     742     915 
   Size:        454     288     173

  Fragments arranged by size: 

                 454     288     173
 
StyI C'CwwG_G

Cuts at:      0     250     915 
   Size:        250     665
 
TatI w'GTAC_w

Cuts at:      0     716     761     915 
   Size:        716      45     154

  Fragments arranged by size: 

                 716     154      45
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     801     915 
   Size:        801     114
 
XmaI C'CCGG_G

Cuts at:      0      65     915 
   Size:         65     850
 
XmnI GAAnn'nnTTC

Cuts at:      0     148     915 
   Size:        148     767
 

 Enzymes that do cut:

     AarI   AflIII     AgeI     AlfI     ApaI    ApaLI     ApoI     AvaI
     BanI    BanII     BcgI     BclI     BlpI Bme1580I     BpmI    BpuEI
     BsaI    BsaAI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BstBI   BstEII    BstYI     BtgI
    BtgZI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I
     FalI     MmeI     MscI     MslI   MspA1I     NcoI    PflMI     PfoI
    PpuMI   PspOMI     PstI    SanDI     SbfI     SfcI     SmaI     SmlI
     StyI     TatI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AhdI
     AleI     AloI    AlwNI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI    BciVI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaBI    BsaHI    BsaXI
    BseRI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrGI   BssHII
    BssSI   BstAPI    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     ZraI
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