Restriction map for
pF1KE0916
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BpuAI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BsrI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 325 GTCTAC GT^[AC][GT]AC
AfaI 414 GTAC GT^AC
AlwNI 520 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 383 AGCGCT AGC^GCT
ApaI 486 GGGCCC GGGCC^C
AspHI 288 GAGCAC G[AT]GC[AT]^C
BclI 170 TGATCA T^GATCA
BfaI 805 CTAG C^TAG
BlpI 183 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 183 GCTGAGC GC^T[ACGT]AGC
BsaBI 129 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 348 CGACCG CG[GA][CT]^CG
BsiHKAI 288 GAGCAC G[AT]GC[AT]^C
BsmAI 58 GTCTC GTCTC(1/5)
BsrFI 221 GCCGGC [GA]^CCGG[CT]
BssHII 549 GCGCGC G^CGCGC
BssSI 424 CACGAG CACGAG(-5/-1)
BstF5I 268 GGATG GGATG(2/0)
CelII 183 GCTGAGC GC^T[ACGT]AGC
Cfr10I 221 GCCGGC [GA]^CCGG[CT]
Eco47III 383 AGCGCT AGC^GCT
FbaI 170 TGATCA T^GATCA
FokI 268 GGATG GGATG(9/13)
HincII 19 GTCAAC GT[CT]^[GA]AC
HindII 19 GTCAAC GT[CT]^[GA]AC
MaeI 805 CTAG C^TAG
MamI 129 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 221 GCCGGC GCC^GGC
NgoMIV 221 GCCGGC G^CCGGC
PflMI 93 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 541 GAGTC GAGTC(4/5)
PstI 418 CTGCAG CTGCA^G
PvuII 523 CAGCTG CAG^CTG
RsaI 414 GTAC GT^AC
SmaI 717 CCCGGG CCC^GGG
TfiI 308 GAATC G^A[AT]TC
Tsp45I 581 GTGAC ^GT[GC]AC
Van91I 93 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 717 CCCGGG C^CCGGG
XmaI 717 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 550 CGCG CG^CG
798 CGCG
BanII 469 GAGCCC G[GA]GC[CT]^C
486 GGGCCC
BseRI 240 GAGGAG GAGGAG(10/8)
466 GAGGAG
BsmFI 509 GGGAC GGGAC(10/14)
772 GGGAC
BstUI 550 CGCG CG^CG
798 CGCG
DdeI 184 CTGAG C^T[ACGT]AG
261 CTCAG
EaeI 356 TGGCCG [CT]^GGCC[GA]
708 CGGCCA
HaeII 383 AGCGCT [GA]GCGC^[CT]
643 AGCGCC
HinfI 308 GAATC G^A[ACGT]TC
541 GAGTC
MaeIII 581 GTGAC ^GT[ACGT]AC
629 GTAAC
MboII 247 GAAGA GAAGA(8/7)
572 TCTTC
MslI 135 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
138 CATCGTGGTG
MvnI 550 CGCG CG^CG
798 CGCG
NlaIII 99 CATG CATG^
672 CATG
SfcI 418 CTGCAG C^T[GA][CT]AG
693 CTACAG
TaqI 431 TCGA T^CGA
575 TCGA
TthHB8I 431 TCGA T^CGA
575 TCGA
XcmI 344 CCACCGACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
738 CCAGCAGAGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AvaI AvaII BbrPI
BbvI BcnI BmyI BpmI BsaAI
BsaJI BsgI BsiYI BslI BsoBI
Bsp1286I BspMI BssKI BstNI Cac8I
CfoI Cfr13I DpnII DraII EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MboI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIV
PmaCI PmlI PpuMI Sau3AI Sau96I
ScrFI SfaNI TseI TspRI