Restriction map for pF1KE0915
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BanII       BbeI        BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaHI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
FbaI        FseI        FspI        HgaI        Hin1I       
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
NspI        PacI        PaeR7I      PflFI       PflMI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AfaI          444  GTAC                 GT^AC
AgeI           33  ACCGGT               A^CCGGT
AlwNI         333  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       163  GAAATGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII          91  GGTCC                G^G[AT]CC
BamHI         728  GGATCC               G^GATCC
BbrPI         709  CACGTG               CAC^GTG
BglII          75  AGATCT               A^GATCT
BmyI          468  GGGCAC               G[AGT]GC[ACT]^C
BsaBI         279  GATAGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI           24  GAGACC               GGTCTC(1/5)
BsmFI         142  GTCCC                GGGAC(10/14)
Bsp1286I      468  GGGCAC               G[AGT]GC[ACT]^C
BspMI         296  ACCTGC               ACCTGC(4/8)
BsrFI          33  ACCGGT               [GA]^CCGG[CT]
BsrI          740  CCAGT                ACTGG(1/-1)
BstBI         380  TTCGAA               TT^CGAA
BstF5I        720  GGATG                GGATG(2/0)
BstXI          11  CCAGCGTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I         33  ACCGGT               [GA]^CCGG[CT]
DraII         498  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DrdI           59  GACTTGGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          122  TGGCCG               [CT]^GGCC[GA]
EarI          148  GAAGAG               CTCTTC(1/4)
EcoO109I      498  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       578  ATGCAT               ATGCA^T
FokI          720  GGATG                GGATG(9/13)
HaeII           3  GGCGCT               [GA]GCGC^[CT]
HincII        697  GTCAAC               GT[CT]^[GA]AC
HindII        697  GTCAAC               GT[CT]^[GA]AC
Ksp632I       148  GAAGAG               CTCTTC(1/4)
MaeIII        743  GTGAC                ^GT[ACGT]AC
MamI          279  GATAGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI          581  CATATG               CA^TATG
NsiI          578  ATGCAT               ATGCA^T
NspV          380  TTCGAA               TT^CGAA
PinAI          33  ACCGGT               A^CCGGT
PmaCI         709  CACGTG               CAC^GTG
PmlI          709  CACGTG               CAC^GTG
RsaI          444  GTAC                 GT^AC
SapI          148  GAAGAGC              GCTCTTC(1/4)
SfuI          380  TTCGAA               TT^CGAA
StuI          629  AGGCCT               AGG^CCT
Tsp45I        743  GTGAC                ^GT[GC]AC
XcmI          740  CCAGTGACAATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          163  GAAATGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII          46  CGCG                 CG^CG
              126  CGCG                
BanI          304  GGCACC               G^G[CT][GA]CC
              469  GGCACC              
BfaI           88  CTAG                 C^TAG
              301  CTAG                
BpmI          228  CTCCAG               CTGGAG(16/14)
              738  CTCCAG              
BsaAI         514  TACGTG               [CT]AC^GT[GA]
              709  CACGTG              
BsaWI          33  ACCGGT               [AT]^CCGG[AT]
              533  ACCGGA              
BsgI           71  GTGCAG               GTGCAG(16/14)
              692  GTGCAG              
BsrDI         252  CATTGC               GCAATG(2/0)
              360  GCAATG              
BstUI          46  CGCG                 CG^CG
              126  CGCG                
HapII          34  CCGG                 C^CGG
              534  CCGG                
HpaII          34  CCGG                 C^CGG
              534  CCGG                
MaeI           88  CTAG                 C^TAG
              301  CTAG                
MaeII         515  ACGT                 A^CGT
              710  ACGT                
MboII         148  GAAGA                GAAGA(8/7)
              270  GAAGA               
MspI           34  CCGG                 C^CGG
              534  CCGG                
MvnI           46  CGCG                 CG^CG
              126  CGCG                
PvuII         221  CAGCTG               CAG^CTG
              407  CAGCTG              
SfaNI         676  GATGC                GCATC(5/9)
              395  GCATC               
TfiI          158  GAATC                G^A[AT]TC
              368  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BsaJI       
BsiYI       BslI        BsmAI       BssKI       BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DsaI        EcoRII      EcoT14I     FauI        
Fnu4HI      HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        MboI        MflI        MnlI        
MspA1I      MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       PleI        Sau3AI      Sau96I      
ScrFI       SfcI        StyI        TaqI        TseI        
TspRI       TthHB8I     XhoII       
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