Restriction map for pF1KE0913
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        Alw44I      
AlwI        AlwNI       AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AvaI        AvrII       BamHI       BanI        
BbeI        BbvCI       BcgI        BciVI       BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        EclXI       
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FseI        HapII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MroI        
MseI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI          214  GAATTT               [GA]^AATT[CT]
AfaI          328  GTAC                 GT^AC
AflIII         87  ACATGT               A^C[GA][CT]GT
AhdI          672  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          214  GAATTT               [GA]^AATT[CT]
AspEI         672  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         701  GGACC                G^G[AT]CC
AviII         725  TGCGCA               TGC^GCA
BanII         376  GAGCCC               G[GA]GC[CT]^C
BbrPI         432  CACGTG               CAC^GTG
BbvI          739  GCAGC                GCAGC(8/12)
BclI           92  TGATCA               T^GATCA
BfaI           25  CTAG                 C^TAG
BpmI          332  CTCCAG               CTGGAG(16/14)
BsaAI         432  CACGTG               [CT]AC^GT[GA]
BsmBI         193  GAGACG               CGTCTC(1/5)
BspHI         290  TCATGA               T^CATGA
BspLU11I       87  ACATGT               A^CATGT
BsrDI         734  GCAATG               GCAATG(2/0)
BstBI         440  TTCGAA               TT^CGAA
Cac8I         229  GCCAGC               GC[ACGT]^[ACGT]GC
DraII         753  GGGGCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      672  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          593  CTCTTC               CTCTTC(1/4)
EcoNI          19  CCTGTGCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      753  GGGGCCT              [GA]G^G[ACGT]CC[CT]
FbaI           92  TGATCA               T^GATCA
FspI          725  TGCGCA               TGC^GCA
Ksp632I       593  CTCTTC               CTCTTC(1/4)
MaeI           25  CTAG                 C^TAG
NspI           87  ACATGT               [GA]CATG^[CT]
NspV          440  TTCGAA               TT^CGAA
PleI           14  GAGTC                GAGTC(4/5)
PmaCI         432  CACGTG               CAC^GTG
PmlI          432  CACGTG               CAC^GTG
PstI          561  CTGCAG               CTGCA^G
RcaI          290  TCATGA               T^CATGA
RsaI          328  GTAC                 GT^AC
SfuI          440  TTCGAA               TT^CGAA
TseI          739  GCAGC                G^C[AT]GC
Tsp509I       215  AATT                 ^AATT
XcmI          257  CCAACATAGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI          510  CCGC                 CCGC(-3/-1)
              204  GCGG                
Aor51HI        55  AGCGCT               AGC^GCT
              299  AGCGCT              
AspHI         346  GTGCTC               G[AT]GC[AT]^C
              571  GAGCAC              
BalI            2  TGGCCA               TGG^CCA
              254  TGGCCA              
BbsI          496  GAAGAC               GAAGAC(2/6)
              481  GTCTTC              
BpuAI         496  GAAGAC               GAAGAC(2/6)
              481  GTCTTC              
BsiHKAI       346  GTGCTC               G[AT]GC[AT]^C
              571  GAGCAC              
BsmFI         700  GGGAC                GGGAC(10/14)
               16  GTCCC               
BstF5I         96  CATCC                GGATG(2/0)
              585  GGATG               
DpnII          93  GATC                 ^GATC
              318  GATC                
EaeI            2  TGGCCA               [CT]^GGCC[GA]
              254  TGGCCA              
Eco47III       55  AGCGCT               AGC^GCT
              299  AGCGCT              
Fnu4HI        204  GCGGC                GC^[ACGT]GC
              739  GCAGC               
FokI           96  CATCC                GGATG(9/13)
              585  GGATG               
HaeII          55  AGCGCT               [GA]GCGC^[CT]
              299  AGCGCT              
ItaI          204  GCGGC                GC^[ACGT]GC
              739  GCAGC               
MboI           93  GATC                 ^GATC
              318  GATC                
MluNI           2  TGGCCA               TGG^CCA
              254  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
              254  TGGCCA              
MwoI          241  GCTCAAGGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              256  GCCAACATAGC         
NdeII          93  GATC                 ^GATC
              318  GATC                
NlaIV         700  GGGACC               GG[ACGT]^[ACGT]CC
              753  GGGGCC              
Sau3AI         93  GATC                 ^GATC
              318  GATC                
SfaNI         616  GATGC                GCATC(5/9)
              689  GCATC               
SfcI          561  CTGCAG               C^T[GA][CT]AG
              641  CTACAG              
SmlI          242  CTCAAG               C^T[CT][GA]AG
              598  CTCAAG              
TaqI          401  TCGA                 T^CGA
              441  TCGA                
TfiI           64  GAATC                G^A[AT]TC
              117  GAATC               
Tsp45I        430  GTCAC                ^GT[GC]AC
              457  GTCAC               
TthHB8I       401  TCGA                 T^CGA
              441  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AluI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BstNI       
CfoI        Cfr13I      DdeI        EcoRII      HaeIII      
HhaI        HinP1I      HinfI       HphI        MaeII       
MaeIII      MboII       MnlI        MslI        MvaI        
NlaIII      Sau96I      ScrFI       TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com